Application and validation of a urinary methadone metabolite (EDDP) immunoassay to monitor methadone compliance.

abstract：:We developed a binary gradient high-performance liquid chromatography (HPLC) method for measuring HbA2 in whole blood samples using a Pharmacia Mono Q column (1 mL) and measurement at 415 nm. The assay requires a simple lysis and centrifugation step before injection onto the column. We found good agreement of results ...

journal_title：Annals of clinical biochemistry

authors： Keevil BG,Maylor PW,Rowlands D

更新日期：1996-05-01 00:00:00

abstract：:Free and total reduced concentrations of D-penicillamine have been measured in the plasma of rheumatoid arthritis patients by HPLC and electro-chemical detection. A reverse-phase ion-pair separation in conjunction with a dual porous graphite electrode satisfied the requirements of robustness, sensitivity, selectivity ...

journal_title：Annals of clinical biochemistry

authors： Webb BA,Richardson SJ,Haslock I

更新日期：1988-03-01 00:00:00

abstract：:A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...

journal_title：Annals of clinical biochemistry

authors： Stewart MJ,Willis RG

更新日期：1975-01-01 00:00:00

abstract：BACKGROUND:Ischaemia-modified albumin (IMA) is a relatively new marker of ischaemia. IMA has not been studied previously in pleural effusions due to congestive heart failure (CHF). The aim of our study was to assess the diagnostic value of IMA in the pleural fluid (PF) and serum for the identification of pleural effusi...

journal_title：Annals of clinical biochemistry

authors： Ozsu S,Gulsoy A,Karahan SC,Mentese A,Nuhoglu I,Ozlu T

更新日期：2011-01-01 00:00:00

abstract：:Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...

journal_title：Annals of clinical biochemistry

authors： Cope G,Nayyar P,Holder R,Brock G,Chapple I

更新日期：2000-09-01 00:00:00

abstract：:The diagnostic performance of a new enzyme linked immunosorbent assay for the cardiac structural protein troponin T in the differential diagnosis of ischaemic cardiac damage was assessed. A well documented set of patients admitted to the coronary care unit of a district general hospital were studied. At a cutoff value...

journal_title：Annals of clinical biochemistry

authors： Collinson PO,Moseley D,Stubbs PJ,Carter GD

更新日期：1993-01-01 00:00:00

abstract：:We have made an evaluation of glycosylated haemoglobin measurements by affinity chromatography in a paediatric population. Age-related reference values for non-diabetic infants and children are reported. A poor correlation (r = 0.24) was found between glycosylated haemoglobin values in 26 insulin-dependent diabetic ch...

journal_title：Annals of clinical biochemistry

authors： van Heyningen C,Hanid TK,Hopkinson I

更新日期：1986-07-01 00:00:00

abstract：:The measurement of carbamylated haemoglobin is a useful indicator of uraemic state during the preceding few weeks in patients with renal failure. In diabetic uraemic patients with hyperglycaemia, glycation of haemoglobin may interfere with its carbamylation, as both reactions involve the free amino groups of the prote...

journal_title：Annals of clinical biochemistry

authors： Hammouda AM,Mady GE

更新日期：2001-03-01 00:00:00

abstract：:Many attempt are now being made by external agencies to assess the quality of assay performance in hormone laboratories. Some of the problems associated with this are discussed and some remedies are proposed. ...

journal_title：Annals of clinical biochemistry

authors： Jeffcoate SL

更新日期：1981-01-01 00:00:00

abstract：BACKGROUND:Since more than 90% of cortisol is bound to protein, serum free cortisol (SFC) may be a more appropriate marker of adrenal status than total cortisol. However, measurement of SFC is technically difficult and calculated SFC may offer a more practical alternative. METHODS:SFC, measured by equilibrium dialysis...

journal_title：Annals of clinical biochemistry

authors： Barlow NL,Holme J,Stockley RA,Clark PM

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:Pregnancy represents a major risk factor for deep vein thrombosis (DVT). Most coagulation/fibrinolysis markers currently utilized change during pregnancy, and therefore they cannot accurately evaluate thrombotic events in pregnancy because the rate of false positive results is high. Fibrin monomer complex (F...

journal_title：Annals of clinical biochemistry

authors： Onishi H,Kaniyu K,Iwashita M,Tanaka A,Watanabe T

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:To know whether metformin improves postprandial hyperglycaemia, we examined the effect of metformin on the glycated albumin (GA) to glycated haemoglobin (HbA1c) ratio (GA/HbA1c ratio) in patients with newly diagnosed type 2 diabetes. METHODS:Metformin and lifestyle interventions were initiated in 18 patient...

journal_title：Annals of clinical biochemistry

authors： Sumitani S,Morita S,Deguchi R,Hirai K,Mukai K,Utsu Y,Miki S,Sato B,Nakamura H,Kasayama S,Koga M

更新日期：2015-01-01 00:00:00

abstract：:A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs. ...

journal_title：Annals of clinical biochemistry

authors： Green B,Cooper JD,Turnell DC

更新日期：1989-07-01 00:00:00

abstract：:The extent of lipoprotein glycation was assessed in 82 patients diagnosed as diabetes mellitus type II. Glycated lipoprotein levels in serum were determined by agarose gel film electrophoresis in 48 non-diabetic control subjects, 56 diabetics with complications and 26 diabetics with no complications. All glycated lipo...

journal_title：Annals of clinical biochemistry

authors： Yeğin A,Ozben T

更新日期：1995-09-01 00:00:00

abstract：:Most UK clinical laboratories use alkaline phosphatase (ALP) methods similar to that proposed by the International Federation of Clinical Chemistry (IFCC), based on the use of 2-amino-2-methyl-1-propanol (AMP) buffer. We present evidence of significant differences in results produced by apparently similar commercial A...

journal_title：Annals of clinical biochemistry

authors： Lamb EJ,Browne M,John WG,Price CP

更新日期：1998-01-01 00:00:00

abstract：:Background Antiphospholipid syndrome is an autoimmune disease characterized by thrombosis, fetal losses and thrombocytopenia associated to antiphospholipid antibodies. They are directed to phospholipids, such as cardiolipins (anticardiolipin) and lupus anticoagulant or to complexes formed by phospholipids and protein ...

journal_title：Annals of clinical biochemistry

authors： Pelusa HF,Pezzarini E,Basiglio CL,Musuruana J,Bearzotti M,Svetaz MJ,Daniele SM,Bottai H,Arriaga SM

更新日期：2017-09-01 00:00:00

abstract：:An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up...

journal_title：Annals of clinical biochemistry

authors： Goldfinch ME,Maguire GA

更新日期：1988-01-01 00:00:00

abstract：:Experience in the use of an in-house urinary free catecholamine assay for the investigation of possible neural crest tumours in a District General Hospital laboratory is described. Elevated excretion of catecholamines and vanillylmandelic acid was found in a number of cases including phaeochromocytoma and neuroblastom...

journal_title：Annals of clinical biochemistry

authors： Davidson DF

更新日期：1987-09-01 00:00:00

abstract：:The mechanisms of hypercalcaemia were assessed in 20 hypercalcaemic patients with breast cancer. Abnormalities suggestive of a PTH-related peptide (PTHrP) mechanism were observed in up to 60% of cases; urinary cyclic adenosine monophosphate (UcAMP) was elevated in nine patients (45%), renal tubular reabsorption of cal...

journal_title：Annals of clinical biochemistry

authors： Gallacher SJ,Fraser WD,Patel U,Logue FC,Soukop M,Boyle IT,Ralston SH

更新日期：1990-11-01 00:00:00

abstract：:This paper summarises the views of the authors on the provision of a prolactin assay service. We discuss the pathophysiology of prolactin secretion and the clinical indications that arise from that. We cover the rather complex issue of the definition of normal and elevated prolactin levels. From these considerations, ...

journal_title：Annals of clinical biochemistry

authors： Jeffcoate SL,Bacon RR,Beastall GH,Diver MJ,Franks S,Seth J

更新日期：1986-11-01 00:00:00

abstract：:It is crucially important to detect subarachnoid haemorrhage (SAH) in all patients in whom it has occurred to select patients for angiography and preventative surgery. A computerized tomography (CT) scan is positive in up to 98% of patients with SAH presenting within 12 h, but is positive in only 50% of those presenti...

journal_title：Annals of clinical biochemistry

authors： Cruickshank A,Auld P,Beetham R,Burrows G,Egner W,Holbrook I,Keir G,Lewis E,Patel D,Watson I,White P,UK NEQAS Specialist Advisory Group for External Quality Assurance of CSF Proteins and Biochemistry.

更新日期：2008-05-01 00:00:00

abstract：:The fasting hyperinsulinaemia characteristic of obesity is due to pancreatic hypersecretion and is accompanied by a corresponding increase in plasma C-peptide concentrations. The exaggerated plasma insulin and C-peptide responses to oral glucose in obese subjects which is greater for insulin than C-peptide suggests th...

journal_title：Annals of clinical biochemistry

authors： Gama R,Marks V

更新日期：1989-09-01 00:00:00

abstract：:A comparison has been made between three methods for measuring capillary blood glucose collected on filter paper, including a new method using a Beckman glucose analyser 2. The methods are suitable for use with capillary blood spots collected onto filter paper for monitoring the control of diabetic patients. Their acc...

journal_title：Annals of clinical biochemistry

authors： Taylor RP,Pennock CA

更新日期：1982-01-01 00:00:00

abstract：BACKGROUND:Our study aims to explore the effect of serum long non-coding RNA (lncRNA) H19 level on the long-term prognosis of endoscopic keyhole surgery or craniotomy for glioma. METHODS:A total of 264 glioma patients were selected. Patients were randomly divided into the Craniotomy-high H19 group, the Craniotomy-low ...

journal_title：Annals of clinical biochemistry

authors： Shen L,Xu M,Wang Z,Yu Z

更新日期：2020-09-01 00:00:00

abstract：:A simple procedure is described for the preparation of a stable precision quality control material for use in the measurement of level of ionised calcium in serum at or near the reference range. Repeat analyses on a Nova 2 ionised calcium analyser of serum pools stored at different temperatures over a period of three ...

journal_title：Annals of clinical biochemistry

authors： Fyffe JA,Jenkins AS,Bolland CJ,Dryburgh FJ,Gardner MD

更新日期：1981-03-01 00:00:00

abstract：BACKGROUND:Golgi protein-73 (GP73) is a newly identified candidate serum marker for liver diseases. The utility of this biomarker remains limited, largely due to the lack of quantitative information. The aims of this study were to quantify serum GP73 (sGP73) in healthy individuals and in patients with liver diseases, a...

journal_title：Annals of clinical biochemistry

authors： Gu Y,Chen W,Zhao Y,Chen L,Peng T

更新日期：2009-01-01 00:00:00

abstract：:A total of 3227 urine specimens were analysed to investigate the applicability of a firefly luciferase assay of microbial adenosine triphosphate (ATP) for the rapid screening of bacteriuria in clinical specimens. Urine sediment, dipslide culture, and three plate cultures were also investigated in the majority of the u...

journal_title：Annals of clinical biochemistry

authors： Ruokonen A,Koskinen M,Leinonen M,Tiilikainen A,Vihko R

更新日期：1982-11-01 00:00:00

abstract：OBJECTIVE:The initial discovery of leptin (1994) has given rise to a substantial number of published studies. This study aimed at identifying the published data on the reference ranges of total, free and bound leptin concentration in the healthy prepubertal population. METHODS:A search was conducted on original Englis...

journal_title：Annals of clinical biochemistry

authors： Venner AA,Doyle-Baker PK,Lyon ME,Fung TS

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Current guidelines document persistent rectal bleeding as an alarm symptom in patients presenting to primary care. We studied whether a faecal immunochemical test could assist in their assessment. METHODS:From December 2015, faecal immunochemical tests were routinely available to primary care when assessing...

journal_title：Annals of clinical biochemistry

authors： Digby J,Strachan JA,McCann R,Steele RJ,Fraser CG,Mowat C

更新日期：2020-07-01 00:00:00

abstract：:An evaluation of the Amerlex system for determination of total thyroxine (T4) and total triiodothyronine (T3) is described. The within- and between-batch precisions were acceptable, and analyses of quality control material and linearity studies demonstrated good accuracy at the clinical decision levels. The correlatio...

journal_title：Annals of clinical biochemistry

authors： Wilke TJ,Turnbull PA

更新日期：1982-03-01 00:00:00