Abstract:
:An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up to 75 mg/L, (3) specificity of the enzyme for unbound bilirubin, as opposed to bound bilirubin, (4) poor precision. Very small absorbance changes leading to poor precision and the potential for interference by conjugated bilirubin meant that the method was unsuitable for patient samples.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Goldfinch ME,Maguire GAdoi
10.1177/000456328802500110subject
Has Abstractpub_date
1988-01-01 00:00:00pages
73-7eissn
0004-5632issn
1758-1001journal_volume
25 ( Pt 1)pub_type
杂志文章abstract::Simultaneous localisation of the isoenzymes of alkaline phosphatase and gamma-glutamyl transferase was carried out on 173 serum samples from patients with hepatobiliary and bone disease to determine (i) if a better discrimination of the underlying pathology could be obtained by such an approach, and (ii) if the pathol...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328001700104
更新日期:1980-01-01 00:00:00
abstract::The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed fo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900111
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:The rapid exclusion of acute myocardial infarction in patients with chest pain can reduce the length of hospital admission, prevent unnecessary diagnostic work-up and reduce the burden on our health-care systems. The combined use of biomarkers that are associated with different pathophysiological aspects of ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215578189
更新日期:2015-09-01 00:00:00
abstract::Clinical biochemists have long known the analytical and clinical limitations of creatinine and creatinine clearance measurement in the assessment of glomerular filtration rate (GFR). This background is reviewed in the article before assessing the utility of cystatin C, the most promising replacement biochemical marker...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/0004563021901847
更新日期:2002-03-01 00:00:00
abstract::An audit of troponin measurement protocols in use in Wales showed significant variations in practice with no consensus on analytical methods, or in the selection of a decision limit for the diagnosis of myocardial damage. Peer review data on assay imprecision at concentrations approaching the analytical limit of detec...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1258/0004563054890006
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:Phlebotomy is a potential cause of preanalytical errors. We have observed phlebotomy in routine practice in a busy Emergency Department, to see how current practice compares with optimal blood sampling. METHODS:Phlebotomy episodes were audited and compared with standard procedures. A computer-based search o...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2011.011099
更新日期:2011-11-01 00:00:00
abstract::The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329903600204
更新日期:1999-03-01 00:00:00
abstract::Serum concentrations of total thyroid hormones, thyroid hormone binding proteins, free thyroid hormones and thyroid stimulating hormone (TSH) were measured in a large group of healthy normal subjects (n = 218) to determine the influence of age and sex on tests of thyroid function. No effect of age on measurements of t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200506
更新日期:1985-09-01 00:00:00
abstract::There is an increasing need for sensitive and specific methods for toxicological screening. Development of a dual-column gas chromatography system for the screening of drugs and metabolites in the plasma of poisoned patients is described. The system has been evaluated using plasma from a well-documented series of pati...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328402100203
更新日期:1984-03-01 00:00:00
abstract::Total serum cholesterol was measured in 1320 normal Saudi children aged 0-14 years. The result was 3.88 (0.83) mmol/L [mean (SD)] and there was no statistical difference between girls and boys. Results were lowest in the 0-4 year age group and highest in the 5-9 year age group. Percentile values were established for t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100407
更新日期:1994-07-01 00:00:00
abstract::A microtiter plate based Dual Analyte enzyme-immunoassay method for the simultaneous measurement of alpha-fetoprotein (AFP) and Free-beta human chorionic gonadotrophin (hCG) was evaluated. This rapid assay, which has application in both Neural Tube Defect screening and Down's screening, shows good precision with betwe...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900403
更新日期:1992-07-01 00:00:00
abstract::In the last few years, the use of enzyme labels in immunoassays has been investigated. The aim of this review is to evaluate critically the role of such labels in clinical biochemistry. Special attention has been given to the problems involved in preparing enzyme labels and the ways in which such labels can be used in...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456327901600162
更新日期:1979-09-01 00:00:00
abstract::Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600214
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:In a network of laboratories analytical variability between instruments, even of the same type, may exist for reasons beyond the control of laboratory staff. Controlling variability is a prerequisite for the application of shared reference ranges and for ensuring the transferability of patient test results. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2010.010005
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Neuroendocrine tumours are slow growing tumours known to secrete a variety of vasoactive peptides which give rise to symptoms of the carcinoid syndrome. The diagnosis and monitoring of patients with neuroendocrine tumours is undertaken in many centres using 24 h urinary measurement of 5-hydroxyindoleacetic a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215613109
更新日期:2016-09-01 00:00:00
abstract::The total cost of a haematology and clinical chemistry laboratory, including external and hospital costs, was studied in a district general hospital in The Netherlands. The main conclusion is that the number of samples analysed is the most important cost-setting factor, but that reduction in the number of requests doe...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328402100206
更新日期:1984-03-01 00:00:00
abstract::In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200126
更新日期:1975-05-01 00:00:00
abstract::There is no definitive treatment protocol for alkaptonuria. A patient with alkaptonuria was treated with ascorbic acid (0.5 g/day) from the age of 4 years. He developed episodes of severe recurrent joint pain at 9.5 years of age after which a protein-restricted diet (1.3 g/kg/day) was started. Protein restriction in c...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303321016268
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:We assessed erythrocyte sedimentation rate and high-sensitivity C-reactive protein concentration in knee osteoarthritis and non-knee osteoarthritis. In addition, we investigated potential relationship between the levels of erythrocyte sedimentation rate and high-sensitivity C-reactive protein with clinical find...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215610142
更新日期:2016-09-01 00:00:00
abstract::Two cases of hypercholesterolaemic patients are presented in whom raised plasma creatine kinase was noted during treatment with statins. The plasma creatine kinase failed to fall following cessation of therapy. Further investigation revealed the aetiology of the raised plasma creatine kinase to be due to previously un...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303322326542
更新日期:2003-09-01 00:00:00
abstract::Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563216674743
更新日期:2017-01-01 00:00:00
abstract::An evaluation of the Amerlex system for determination of total thyroxine (T4) and total triiodothyronine (T3) is described. The within- and between-batch precisions were acceptable, and analyses of quality control material and linearity studies demonstrated good accuracy at the clinical decision levels. The correlatio...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328201900208
更新日期:1982-03-01 00:00:00
abstract::Auto-antibodies to oxidized low-density lipoprotein (ox-LDL) are thought to play a pivotal role in the pathogenesis of atherosclerosis. This study investigates the value of auto-antibodies to ox-LDL as a predictive marker of atherosclerosis in patients with both homozygous and heterozygous familial hypercholesterolaem...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899177
更新日期:2000-03-01 00:00:00
abstract::An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328201900305
更新日期:1982-05-01 00:00:00
abstract::Parenteral nutrition is a well-established technique for feeding patients with intestinal failure and other conditions which render enteral feeding impossible. Biochemical tests are required in the initial assessment of these patients, for monitoring their response to treatment and for the detection and management of ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456328702400401
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:To investigate the reasons why general practitioners (GPs) request rheumatoid factor (RF) assays, we studied 200 consecutive requests for RF from general practice in 1995. METHOD:By means of an audit questionnaire, we studied 100 negative, 50 positive and 50 borderline RF results and compared these with the...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303763046049
更新日期:2003-03-01 00:00:00
abstract::A number of cardiac interventional procedures are available for the treatment of angina, including percutaneous transluminal coronary angioplasty (PTCA), stent insertion and rotational atherectomy (RA). Variable degrees of myocardial cell injury during PTCA and stent insertion have been observed, based on rises in cre...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001900075
更新日期:2000-11-01 00:00:00
abstract::ISO 22870 standards require protocols for performance of internal quality control for all point-of-care testing devices and training of users in its theory and practice. However, the unique setting of point-of-care testing (i.e. processes conducted by non-scientific users) means that laboratory internal quality contro...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563215615148
更新日期:2016-03-01 00:00:00
abstract::Serum retinol-binding protein (RBP), with a biological half-life of less than 12 h, is a useful indicator of liver or kidney dysfunction. An automated immunoturbidimetric assay for the measurement of RBP has been developed using the Cobas-BIO centrifugal analyser and commercially available materials. Serum samples wit...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500309
更新日期:1988-05-01 00:00:00
abstract::Adequate control of performance in immunoassays of maternal serum alpha-fetoprotein is an essential part of screening for neural tube defects. Information derived from external quality assessment schemes may be of limited value in monitoring individual laboratory performance and stresses the need for proper internal q...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328001700611
更新日期:1980-11-01 00:00:00