Acidification and urine calcium: is it a preanalytical necessity?

abstract：BACKGROUND:Supported liquid extraction (SLE) techniques are relatively new compared to other sample preparation approaches such as solid phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation (PPE). We investigated the use of SLE as an alternative to SPE for the liquid chromatography tandem ma...

journal_title：Annals of clinical biochemistry

authors： Owen LJ,Keevil BG

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:Apolipoprotein concentrations in reflex tears from healthy and noninsulin-dependent diabetes mellitus (NIDDM) subjects were measured and correlated with the stage of diabetic retinopathy. METHODS AND RESULTS:The apolipoprotein A-I (apo A-I) concentrations in the tears from NIDDM patients with retinopathy we...

journal_title：Annals of clinical biochemistry

authors： Kawai S,Nakajima T,Hokari S,Komoda T,Kawai K

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:Measurement of serum natriuretic peptides is recommended in patients with suspected heart failure. Assays for N-terminal pro-B-type natriuretic peptide (NT-proBNP) are available on several platforms and can be measured in serum or heparinized plasma. Siemens Healthcare Diagnostics do not recommend the use of...

journal_title：Annals of clinical biochemistry

authors： Peake RW,Turner HE,Leaper W,Deans KA,Hannah A,Croal BL

更新日期：2012-09-01 00:00:00

abstract：:Direct assays have been described for the estimation of progesterone in serum and saliva. Both methods showed good inter-assay precision over a year, and the serum assay is now being used routinely in clinical laboratories. A highly significant correlation was obtained for progesterone concentrations in matched serum ...

journal_title：Annals of clinical biochemistry

authors： Webley GE,Edwards R

更新日期：1985-11-01 00:00:00

abstract：:Background The clinical utility of serum thyroglobulin in the follow-up of patients with differentiated thyroid carcinoma may be compromised by the presence of endogenous antithyroglobulin antibodies. To prevent interference by antithyroglobulin antibodies several groups have developed real-time PCR-based assays for q...

journal_title：Annals of clinical biochemistry

authors： Feddersen S,Bastholt L,Pedersen SM

更新日期：2017-09-01 00:00:00

abstract：:A bloodspot assay for testosterone has been developed using an antiserum raised against testosterone-3-carboxymethyloxime-bovine serum albumin (T-3 CMO-BSA) conjugate and 125I-T-3CMO-histamine tracer. The method has a detection limit of 0.4 nmol/L with between-batch precision of less than 15% for testosterone values b...

journal_title：Annals of clinical biochemistry

authors： Walker J,Hughes IA,Wood PJ

更新日期：1999-07-01 00:00:00

abstract：:Serum retinol-binding protein (RBP), with a biological half-life of less than 12 h, is a useful indicator of liver or kidney dysfunction. An automated immunoturbidimetric assay for the measurement of RBP has been developed using the Cobas-BIO centrifugal analyser and commercially available materials. Serum samples wit...

journal_title：Annals of clinical biochemistry

authors： Bankson DD,Rifai N,Silverman LM

更新日期：1988-05-01 00:00:00

abstract：BACKGROUND:Refeeding syndrome (RS) is a potentially fatal condition that can occur following the re-introduction of nutrition after a period of starvation. Hypophosphataemia following the reintroduction of nutrition is often the only reliable biochemical marker of RS. Refeeding index (RI) generated from baseline insuli...

journal_title：Annals of clinical biochemistry

authors： Goyale A,Ashley SL,Taylor DR,Elnenaei MO,Alaghband-Zadeh J,Sherwood RA,le Roux CW,Vincent RP

更新日期：2015-01-01 00:00:00

abstract：:A simple procedure is described for the preparation of a stable precision quality control material for use in the measurement of level of ionised calcium in serum at or near the reference range. Repeat analyses on a Nova 2 ionised calcium analyser of serum pools stored at different temperatures over a period of three ...

journal_title：Annals of clinical biochemistry

authors： Fyffe JA,Jenkins AS,Bolland CJ,Dryburgh FJ,Gardner MD

更新日期：1981-03-01 00:00:00

abstract：BACKGROUND:Current guidelines document persistent rectal bleeding as an alarm symptom in patients presenting to primary care. We studied whether a faecal immunochemical test could assist in their assessment. METHODS:From December 2015, faecal immunochemical tests were routinely available to primary care when assessing...

journal_title：Annals of clinical biochemistry

authors： Digby J,Strachan JA,McCann R,Steele RJ,Fraser CG,Mowat C

更新日期：2020-07-01 00:00:00

abstract：BACKGROUND:About 10 years ago, a survey showed significant deficiencies in the quality of gastrointestinal investigations performed by UK laboratories. Subsequently, several clinical guidelines have been developed and external quality assessment schemes introduced. This survey was performed to assess whether earlier de...

journal_title：Annals of clinical biochemistry

authors： Duncan A,Hill PG

更新日期：2007-03-01 00:00:00

abstract：:Eleven unfit bank executives in sedentary employment exercised daily for 10 weeks according to the Canadian Air-Force 5BX Exercise Programme. Over this period no changes were observed in the glucose or insulin responses to a standard breakfast or to intravenous glucose, or in the glucose response to intravenous insuli...

journal_title：Annals of clinical biochemistry

authors： Moore RA,Penfold WA,Simpson RD,Simpson RW,Mann JI,Turner RC

更新日期：1979-03-01 00:00:00

abstract：:A patient with sepsis and jaundice was admitted for diagnosis and treatment. Associated biochemical changes included increased C-reactive protein, conjugated bilirubin and gamma-glutamyltransferase, the duration of which was protracted. High urine coproporphyrin isomer-1 and immunostaining of liver tissue suggested Du...

journal_title：Annals of clinical biochemistry

authors： Devgun MS,El-Nujumi AM,O'Dowd GJ,Barbu V,Poupon R

更新日期：2012-11-01 00:00:00

abstract：:The effect of progestins on intracellular corticosteroid-binding globulin (CBG) mRNA expression in an endometrial cancer cell line (Ishikawa) was examined in an attempt to understand the biological effects of high-dose progestins in the treatment of well-differentiated uterine endometrial cancers. Oestradiol-17 beta (...

journal_title：Annals of clinical biochemistry

authors： Misao R,Nakanishi Y,Fujimoto J,Tamaya T

更新日期：1998-09-01 00:00:00

abstract：:A number of cardiac interventional procedures are available for the treatment of angina, including percutaneous transluminal coronary angioplasty (PTCA), stent insertion and rotational atherectomy (RA). Variable degrees of myocardial cell injury during PTCA and stent insertion have been observed, based on rises in cre...

journal_title：Annals of clinical biochemistry

authors： Harris BM,Nageh T,Marsden JT,Thomas MR,Sherwood RA

更新日期：2000-11-01 00:00:00

abstract：:Auto-antibodies to oxidized low-density lipoprotein (ox-LDL) are thought to play a pivotal role in the pathogenesis of atherosclerosis. This study investigates the value of auto-antibodies to ox-LDL as a predictive marker of atherosclerosis in patients with both homozygous and heterozygous familial hypercholesterolaem...

journal_title：Annals of clinical biochemistry

authors： Paiker JE,Raal FJ,von Arb M

更新日期：2000-03-01 00:00:00

abstract：:Haptoglobin is an acute-phase protein with three major phenotypes: Hp 1-1, Hp 2-1 and Hp 2-2. Studies have shown that functional differences between these phenotypes have important consequences in a number of pathological disorders (e.g. cardiovascular disease, autoimmune disorders, infectious disease), making phenoty...

journal_title：Annals of clinical biochemistry

authors： Wassell J,Keevil B

更新日期：1999-09-01 00:00:00

abstract：:Background Accumulating evidence shows an over-abundance of Fusobacterium nucleatum in colorectal tumour tissues. Although stool DNA testing of Fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting Fusobacterium nucleatum in stool by conventional metho...

journal_title：Annals of clinical biochemistry

authors： Suehiro Y,Sakai K,Nishioka M,Hashimoto S,Takami T,Higaki S,Shindo Y,Hazama S,Oka M,Nagano H,Sakaida I,Yamasaki T

更新日期：2017-01-01 00:00:00

abstract：:The presence of antibodies in some patients' serum has long been known to be a potential source of interference in immunoassays, as shown by numerous case reports. These often appear after the introduction of a new analyte (e.g. troponin) and then decrease in number as the topic becomes exhausted. This highlights the ...

journal_title：Annals of clinical biochemistry

authors： Ismail AA,Walker PL,Cawood ML,Barth JH

更新日期：2002-07-01 00:00:00

abstract：:The Poser criteria for diagnosing multiple sclerosis (MS) includes clinical, paraclinical and laboratory information. We studied the influence of cerebrospinal fluid (CSF) biochemistry results on the categorisation of patients with suspected MS. A retrospective study was made of 138 patients who had CSF samples sent o...

journal_title：Annals of clinical biochemistry

authors： Ratnaike S,Kilpatrick T,Tress B,Davis S,Kilpatrick C,Byron K,Deam D

更新日期：1990-05-01 00:00:00

abstract：:The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed fo...

journal_title：Annals of clinical biochemistry

authors： Starcher B,Scott M

更新日期：1992-01-01 00:00:00

abstract：:The immunosuppressive agent mycophenolate mofetil is a prodrug for the active compound mycophenolic acid. Following organ transplantation, it is used mostly in combination with other immunosuppressive drugs, but it has also been used as primary therapy. Although in the pivotal clinical studies the drug was given in fi...

journal_title：Annals of clinical biochemistry

authors： Holt DW

更新日期：2002-05-01 00:00:00

abstract：:A simultaneous deterioration in performance of several radioimmunoassays suggested that equipment was malfunctioning. Replicate counts from an automated gamma counter showed unacceptable variation during a 24-hour period; this variation was detected using Trigg's technique, a sensitive method for trend analysis, and w...

journal_title：Annals of clinical biochemistry

authors： Challand GS,Cox L

更新日期：1978-03-01 00:00:00

abstract：:We have studied 46 patients, 30 men and 16 women, with type 2 (non-insulin-dependent) diabetes in a follow-up period of 6-52 months (mean 30 months). The patients were consecutively entered in the study from the out-patient diabetic clinic. None had urinary tract infections nor proteinuria at entry. Investigations wer...

journal_title：Annals of clinical biochemistry

authors： Friis T,Pedersen LR

更新日期：1997-05-01 00:00:00

abstract：BACKGROUND:Glycated haemoglobin (HbA1c) concentration is lower relative to glyacemic control in diabetic patients on haemodialysis. However, it is unknown as to whether this is also true for diabetic patients with end-stage renal disease but not on haemodialysis. METHODS:Correlations between HbA1c or glycated albumin ...

journal_title：Annals of clinical biochemistry

authors： Shima K,Chujo K,Yamada M,Komatsu M,Noma Y,Mizuguchi T

更新日期：2012-01-01 00:00:00

abstract：:The development and evaluation in routine clinical practice of a simple, rapid, high-throughput, immunoradiometric assay (IRMA) for alpha-foetoprotein (AFP) in serum and amniotic fluid is described. The assay uses readily available reagents; a radioiodinated mouse monoclonal antibody produced by the authors and the im...

journal_title：Annals of clinical biochemistry

authors： Stevenson JD,Chapman RS,Perry B,Logue FC

更新日期：1987-07-01 00:00:00

abstract：:Erythrocyte and fasting plasma magnesium were assayed by a dyebinding colorimetric method using the metallochromic dye, Calmagite, in 51 diabetic Nigerians and 25 controls. Erythrocyte magnesium concentrations were similar in the diabetics and the controls. Fasting plasma magnesium concentrations were significantly lo...

journal_title：Annals of clinical biochemistry

authors： Olukoga AO,Erasmus RT,Adewoye HO

更新日期：1989-01-01 00:00:00

abstract：:A 19-year-old man who developed extensive oesophageal lye (Alkali) stricture and received long-term enteral nutrition (eight months) with a jejunostomy tube developed macrocytic anaemia (Hb: 41 g/L) with leucopenia (white blood cell [WBC]: 3.0 x 10(9)/L). The patient's serum vitamin B12, folate, iron and liver functio...

journal_title：Annals of clinical biochemistry

authors： Jayakumar S,Micallef-Eynaud PD,Lyon TD,Cramb R,Jilaihawi AN,Prakash D

更新日期：2005-05-01 00:00:00

abstract：BACKGROUND:Haemoglobin A1c (HbA1c) is the only measure of glycaemic control used for many patients with diabetes, but it has limitations and might sometimes be misleading. HbA(1c) concentrations are influenced by conditions that alter red-cell life and there is evidence that biochemical variation in intracellular glyca...

journal_title：Annals of clinical biochemistry

authors： Macdonald DR,Hanson AM,Holland MR,Singh BM

更新日期：2008-07-01 00:00:00

abstract：:Quadriceps muscle specimens from autopsy of 28 neonates (gestational age 25-42 weeks) were investigated to determine pyruvate and malate oxidation rates and several enzymes of the mitochondrial oxidative process. In general, the levels of all mitochondrial parameters measured, including carnitine levels, were lower in...

journal_title：Annals of clinical biochemistry

authors： Sperl W,Sengers RC,Trijbels JM,Ruitenbeek W,Doesburg WH,Smeitink JA,Kollée LA,Boon JM

更新日期：1992-11-01 00:00:00