Supported liquid extraction as an alternative to solid phase extraction for LC-MS/MS aldosterone analysis?

abstract：:We evaluated a new immunoselective enzyme assay of bone-specific alkaline phosphatase (ALP). The monoclonal antibody used in this assay was raised against purified bone-specific ALP obtained from SAOS-2 human osteosarcoma cell line. Calibration was based on the enzyme's own activity. The relative activity of the antib...

journal_title：Annals of clinical biochemistry

authors： Hata K,Tokuhiro H,Nakatsuka K,Miki T,Nishizawa Y,Morii H,Miura M

更新日期：1996-03-01 00:00:00

abstract：BACKGROUND:Combined lipid and glucose point-of-care testing (POCT) devices could facilitate widespread population screening for cardiovascular disease (CVD) and diabetes as part of the NHS Vascular Risk Assessment and Management Program (NHS Health Checks). An evaluation of the Cholestech LDX and CardioChek PA POCT ana...

journal_title：Annals of clinical biochemistry

authors： Whitehead SJ,Ford C,Gama R

更新日期：2014-01-01 00:00:00

abstract：:This paper describes some factors to which attention should be paid when atrial natriuretic peptide (ANP) values are to be interpreted. Changing from the supine to the sitting position decreased ANP levels, while no further decrease was observed when the upright position was assumed. On the other hand, plasma renin ac...

journal_title：Annals of clinical biochemistry

authors： Tan AC,Kloppenborg PW,Benraad TJ

更新日期：1989-11-01 00:00:00

abstract：BACKGROUND:Individuals who are unable to metabolize the short-acting muscle relaxant succinylcholine due to abnormal cholinesterase activity are currently investigated via spectrophotometry using artificial substrates and enzyme inhibitors. Methods have been described using succinylcholine as substrate but with measure...

journal_title：Annals of clinical biochemistry

authors： Spencer JD,Rushton D,Allen KR

更新日期：2010-05-01 00:00:00

abstract：:Insulin resistance is a common condition, recognized to be a central feature of the metabolic syndrome, and strongly associated with an increased risk of cardiovascular disease and diabetes. The quantitative assessment of insulin sensitivity is not used for routine clinical purposes, but the emerging importance of ins...

journal_title：Annals of clinical biochemistry

authors： Borai A,Livingstone C,Ferns GA

更新日期：2007-07-01 00:00:00

abstract：:Multiple myeloma (MM) is a plasma cell dyscrasia characterized by the malignant proliferation of a plasma cell clone that produces a monoclonal immunoglobulin. Diagnosis and management of patients with monoclonal gammopathies depend on accurate identification and characterization of monoclonal proteins. We present a 6...

journal_title：Annals of clinical biochemistry

authors： Aksungar FB,Ayer M,Serteser M,Coskun A,Unsal I

更新日期：2014-09-01 00:00:00

abstract：:We have studied 46 patients, 30 men and 16 women, with type 2 (non-insulin-dependent) diabetes in a follow-up period of 6-52 months (mean 30 months). The patients were consecutively entered in the study from the out-patient diabetic clinic. None had urinary tract infections nor proteinuria at entry. Investigations wer...

journal_title：Annals of clinical biochemistry

authors： Friis T,Pedersen LR

更新日期：1997-05-01 00:00:00

abstract：BACKGROUND:The relationship between plasma angiopoietin-like protein 3 (ANGPTL3), and lipoprotein lipase (LPL) activity and hepatic triglyceride lipase (HTGL) activity has not been investigated in the metabolism of remnant lipoproteins (RLPs) and high-density lipoprotein (HDL) in human plasma. METHODS:ANGPTL3, LPL act...

journal_title：Annals of clinical biochemistry

authors： Nakajima K,Kobayashi J,Mabuchi H,Nakano T,Tokita Y,Nagamine T,Imamura S,Ai M,Otokozawa S,Schaefer EF

更新日期：2010-09-01 00:00:00

abstract：:Plasma C1 inhibitor (C1-INH) was determined in 64 patients with different malignant diseases and in 58 healthy persons. C1-INH antigen concentration was measured by radial immunodiffusion (RID), whereas its functional activity was assayed with chromogenic substrate. Within-run and day-to-day precision of both methods ...

journal_title：Annals of clinical biochemistry

authors： Starcević D,Jelić-Ivanović Z,Kalimanovska V

更新日期：1991-11-01 00:00:00

abstract：:Direct assays have been described for the estimation of progesterone in serum and saliva. Both methods showed good inter-assay precision over a year, and the serum assay is now being used routinely in clinical laboratories. A highly significant correlation was obtained for progesterone concentrations in matched serum ...

journal_title：Annals of clinical biochemistry

authors： Webley GE,Edwards R

更新日期：1985-11-01 00:00:00

abstract：:The effect of ambient temperature on the analytical and clinical performance of a glucose meter was examined. A total of 114 venous whole blood samples were analysed for glucose by a reference method, and by a glucose meter at 21-22 degrees C, room temperatures, 26-27 degrees C and 33-34 degrees C. Glucose meter readi...

journal_title：Annals of clinical biochemistry

authors： Nawawi H,Sazali BS,Kamaruzaman BH,Yazid TN,Jemain AA,Ismail F,Khalid BA

更新日期：2001-11-01 00:00:00

abstract：BACKGROUND:Recent NICE Guidelines have emphasized the need to have in place by 2007 the capability of offering screening to all women in the first trimester using a combination of maternal age with the ultrasound marker nuchal translucency thickness (NT) and the maternal serum biochemical markers free beta-hCG and preg...

journal_title：Annals of clinical biochemistry

authors： Spencer K

更新日期：2005-01-01 00:00:00

abstract：:Tiaprofenic acid is a non-steroidal anti-inflammatory drug which also has hypouriceamic effect. Studies involving 10 healthy volunteers were designed to investigate the mode of this effect. We postulate that the site of action of tiaprofenic acid is at the cell membrane, the mechanism being an interference with the tr...

journal_title：Annals of clinical biochemistry

authors： Lyfar V,Fernandes L,Thornton E,Cook JG

更新日期：1988-11-01 00:00:00

abstract：:Requirements for information technology in pathology now extend well beyond the provision of purely analytical data. With the aim of achieving seamless integration of laboratory data into the total clinical pathway, "informatics"--the art and science of turning data into useful information--is becoming increasingly im...

journal_title：Annals of clinical biochemistry

authors： Jones R,O'Connor J

更新日期：2004-05-01 00:00:00

abstract：:A patient with sepsis and jaundice was admitted for diagnosis and treatment. Associated biochemical changes included increased C-reactive protein, conjugated bilirubin and gamma-glutamyltransferase, the duration of which was protracted. High urine coproporphyrin isomer-1 and immunostaining of liver tissue suggested Du...

journal_title：Annals of clinical biochemistry

authors： Devgun MS,El-Nujumi AM,O'Dowd GJ,Barbu V,Poupon R

更新日期：2012-11-01 00:00:00

abstract：:Guaiac-based faecal occult blood tests (gFOBT) are still used in asymptomatic population bowel screening programmes but are being replaced by faecal immunochemical tests (FIT) for haemoglobin. gFOBT have many well-documented disadvantages and there is little evidence for their use in assessment of the symptomatic. Man...

journal_title：Annals of clinical biochemistry

authors： Fraser CG

更新日期：2012-11-01 00:00:00

abstract：:In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...

journal_title：Annals of clinical biochemistry

authors： Moorhead JF,Varghese Z,Tatler GL,Baillod RA,Wills MR

更新日期：1975-05-01 00:00:00

abstract：:Background Red blood cell distribution width (RDW) and neutrophil to lymphocyte ratio (NLR) have been reported to be associated with outcomes of acute cerebral infarction. However, their prognostic value in patients with subarachnoid haemorrhage (SAH) remains largely unknown. The aim of this study was to investigate t...

journal_title：Annals of clinical biochemistry

authors： Huang YL,Han ZJ,Hu ZD

更新日期：2017-11-01 00:00:00

abstract：:This paper critically examines the usefulness of serum albumin measurement in the light of current laboratory practice and knowledge of the pathophysiology of albumin metabolism. The main conclusions and recommendations are as follows: (i) Albumin measurement forms a limited, but useful part of the investigation of li...

journal_title：Annals of clinical biochemistry

authors： Whicher J,Spence C

更新日期：1987-11-01 00:00:00

abstract：:A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protecte...

journal_title：Annals of clinical biochemistry

authors： Pickard CE,Stewart AD,Hartley R,Lucock MD

更新日期：1986-07-01 00:00:00

abstract：BACKGROUND:Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipept...

journal_title：Annals of clinical biochemistry

authors： Ghodsi R,Kheirouri S,Nosrati R

更新日期：2019-01-01 00:00:00

abstract：:The effect of oral vitamin E supplementation on the oxido-reductive status of red blood cells in normal mice and those subject to oxidative stress by chronic administration of the anti-tumour drug Adriamycin was investigated. Mice were randomly separated into three groups of 20 animals each and maintained on diets ide...

journal_title：Annals of clinical biochemistry

authors： Thabrew MI,Samarawickrema N,Chandrasena LG,Jayasekera S

更新日期：1999-03-01 00:00:00

abstract：:The magnitude of the inflammatory response to surgery depends on the degree of injury during surgical procedures. Laparoscopic techniques are generally associated with less postoperative pain and shorter hospital stay compared with open procedures, presumably due to less tissue injury and reduced inflammatory response...

journal_title：Annals of clinical biochemistry

authors： Labib M,Palfrey S,Paniagua E,Callender R

更新日期：1997-09-01 00:00:00

abstract：:The mechanisms of hypercalcaemia were assessed in 20 hypercalcaemic patients with breast cancer. Abnormalities suggestive of a PTH-related peptide (PTHrP) mechanism were observed in up to 60% of cases; urinary cyclic adenosine monophosphate (UcAMP) was elevated in nine patients (45%), renal tubular reabsorption of cal...

journal_title：Annals of clinical biochemistry

authors： Gallacher SJ,Fraser WD,Patel U,Logue FC,Soukop M,Boyle IT,Ralston SH

更新日期：1990-11-01 00:00:00

abstract：:The diagnostic performance of a new enzyme linked immunosorbent assay for the cardiac structural protein troponin T in the differential diagnosis of ischaemic cardiac damage was assessed. A well documented set of patients admitted to the coronary care unit of a district general hospital were studied. At a cutoff value...

journal_title：Annals of clinical biochemistry

authors： Collinson PO,Moseley D,Stubbs PJ,Carter GD

更新日期：1993-01-01 00:00:00

abstract：:The application of a DC plasma emission spectrometer to the measurement of copper, zinc and magnesium in serum and urine is reported. Each assay requires only a simple dilution in 1% nitric acid. Comparisons with standard atomic absorption techniques showed good analytical agreement, especially for copper and magnesiu...

journal_title：Annals of clinical biochemistry

authors： Roberts NB,Fairclough D,McLoughlin S,Taylor WH

更新日期：1985-09-01 00:00:00

abstract：:Measurement of urinary free cortisol using the Bayer Automated Chemiluminescent System (ACS:180 PLUS) was evaluated and compared with an in-house extraction radioimmunoassay (RIA). Inter-assay coefficients of variation were acceptable, being respectively 5.3%, 4.8% and 3.8% at 141, 406 and 942 nmol/L (n=20) for the AC...

journal_title：Annals of clinical biochemistry

authors： Kennedy DM,Selby C,Lawson N

更新日期：2000-07-01 00:00:00

abstract：BACKGROUND:The rapid exclusion of acute myocardial infarction in patients with chest pain can reduce the length of hospital admission, prevent unnecessary diagnostic work-up and reduce the burden on our health-care systems. The combined use of biomarkers that are associated with different pathophysiological aspects of ...

journal_title：Annals of clinical biochemistry

authors： Jacobs LH,van Borren M,Gemen E,van Eck M,van Son B,Glatz JF,Daniels M,Kusters R

更新日期：2015-09-01 00:00:00

abstract：:A total of 1381 urine specimens were screened using a Microgenics CEDIA urinary primary methadone metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; EDDP) immunoassay (cut-off calibrator concentration of 100 microg/L) in combination with a Dade Behring EMIT urinary methadone immunoassay (cut-off calibrator...

journal_title：Annals of clinical biochemistry

authors： George S,Parmar S,Meadway C,Braithwaite RA

更新日期：2000-05-01 00:00:00

abstract：:There is an increasing need for sensitive and specific methods for toxicological screening. Development of a dual-column gas chromatography system for the screening of drugs and metabolites in the plasma of poisoned patients is described. The system has been evaluated using plasma from a well-documented series of pati...

journal_title：Annals of clinical biochemistry

authors： Jarvie DR,Simpson D

更新日期：1984-03-01 00:00:00