Evaluation and clinical application of a two-site immunoradiometric assay for alpha-1-foetoprotein using readily available reagents.

Abstract:

:The development and evaluation in routine clinical practice of a simple, rapid, high-throughput, immunoradiometric assay (IRMA) for alpha-foetoprotein (AFP) in serum and amniotic fluid is described. The assay uses readily available reagents; a radioiodinated mouse monoclonal antibody produced by the authors and the immunoglobulin G (IgG) fraction of a polyclonal sheep anti-human-AFP serum covalently linked to Sepharose CL-4B. Both antibodies are distributed by the Scottish Antibody Production Unit (Law Hospital, Carluke). The results of an extensive comparative trial between the IRMA and a radioimmunoassay (RIA) are presented. A high-dose 'hook' effect is not a problem in this system. Quantitative recovery was obtained, independent of the assay diluent. The IRMA has high sensitivity and a wide working range. Improved assay precision allowed a clinically significant change in the intervention limits.

journal_name

Ann Clin Biochem

authors

Stevenson JD,Chapman RS,Perry B,Logue FC

doi

10.1177/000456328702400411

subject

Has Abstract

pub_date

1987-07-01 00:00:00

pages

411-8

eissn

0004-5632

issn

1758-1001

journal_volume

24 ( Pt 4)

pub_type

杂志文章
  • Establishment of reference intervals for aldosterone and renin in a Caucasian population using the newly developed Immunodiagnostic Systems specialty immunoassay automated system.

    abstract:BACKGROUND:Measurement of aldosterone and/or renin is essential to aid the differential diagnosis of secondary hypertension, guide strategy for therapeutic management of hypertension and assess adequacy of mineralocorticoid replacement. AIM:The objective was to establish normative data for aldosterone and renin using ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563215603401

    authors: O'Shea P,Brady JJ,Gallagher N,Dennedy MC,Fitzgibbon M

    更新日期:2016-05-01 00:00:00

  • A simple, rapid and cheap radioimmunoassay for plasma digoxin.

    abstract::A radioimmoassay for digoxin is described which uses commercially available reagents. Two assay procedures are used, one for batch assays, and another, which needs a 5 minute incubation only, for 'stat' assays. The methods are shown to be simple, rapid, precise and inexpensive and the batch procedure compared well wit...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328502200216

    authors: Moore RA,Turner M,Cousins J,Gales M

    更新日期:1985-03-01 00:00:00

  • A novel five-microlitre capillary system for home glucose monitoring.

    abstract::Improvement in the control of diabetic patients is aided by a knowledge of blood glucose levels during a 'normal' (non-hospitalised) day. We have devised a 5 microliter capillary tube collection system as a 'kit' for home use by diabetics. Blood collected into 5 microliter capillary tubes is washed into a protein prec...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328101800507

    authors: Williams CP,Davies GK,Child DF

    更新日期:1981-09-01 00:00:00

  • The effect of eccentric exercise on serum creatine kinase activity in different ethnic groups.

    abstract::Eccentric exercise causes release of muscle creatine kinase (CK) 3-4 days after exercise. Racial variation in basal serum CK has been reported but the reasons for this are unknown. We studied 30 subjects of different ethnic origin (Caucasian, Afro-Caribbean, Asian) before and after eccentric exercise. Basal serum CK w...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329603300407

    authors: Sherwood RA,Lambert A,Newham DJ,Wassif WS,Peters TJ

    更新日期:1996-07-01 00:00:00

  • Acquired copper deficiency following prolonged jejunostomy feeds.

    abstract::A 19-year-old man who developed extensive oesophageal lye (Alkali) stricture and received long-term enteral nutrition (eight months) with a jejunostomy tube developed macrocytic anaemia (Hb: 41 g/L) with leucopenia (white blood cell [WBC]: 3.0 x 10(9)/L). The patient's serum vitamin B12, folate, iron and liver functio...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/0004563053857879

    authors: Jayakumar S,Micallef-Eynaud PD,Lyon TD,Cramb R,Jilaihawi AN,Prakash D

    更新日期:2005-05-01 00:00:00

  • Influence of acetate and bicarbonate dialysate on blood short- and long-chain acyl carnitine in adult pyelonephritis patients.

    abstract::The effect of acetate and bicarbonate haemodialysis (HD) on the concentrations of erythrocyte, whole-blood and plasma total carnitine (TC), free carnitine (FC), short- (SC) and long-chain acylcarnitine (LC) and acylcarnitine (AC) as well as the ratio of AC to FC was investigated in 30 healthy subjects (15 men and 15 w...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329903600106

    authors: Alhomida AS

    更新日期:1999-01-01 00:00:00

  • A simple automated colorimetric method for determination of N-acetyl- beta-D-glucosaminidase.

    abstract::An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328201900305

    authors: Moore JC,Morris JE

    更新日期:1982-05-01 00:00:00

  • Pneumatic tube system induced haemolysis: assessing sample type susceptibility to haemolysis.

    abstract:BACKGROUND:The pneumatic tube system (PTS) has been implicated in inducing haemolysis. It is not known whether certain sample types are more susceptible to haemolysis than others. We assessed the level of haemolysis in commonly used sample types in the clinical biochemistry department when transported through the PTS. ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/000456304323019631

    authors: Sodi R,Darn SM,Stott A

    更新日期:2004-05-01 00:00:00

  • Comparison of biochemical with radiological findings in renal osteodystrophy.

    abstract::In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456327501200126

    authors: Moorhead JF,Varghese Z,Tatler GL,Baillod RA,Wills MR

    更新日期:1975-05-01 00:00:00

  • Faecal elastase 1: a marker of exocrine pancreatic insufficiency in cystic fibrosis.

    abstract::Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329903600606

    authors: Phillips IJ,Rowe DJ,Dewar P,Connett GJ

    更新日期:1999-11-01 00:00:00

  • Sodium measurement: effects of differing sampling and analytical methods.

    abstract:BACKGROUND:It is recommended that children receiving intravenous fluids should have frequent biochemical monitoring, in some situations 4-6 hourly. Small changes in sodium must be detected, requiring very high precision from sodium analyses. Some children are monitored using venous blood analysed by indirect ion-select...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/000456306778904560

    authors: Loughrey CM,Hanna EV,McDonnell M,Archbold GP

    更新日期:2006-11-01 00:00:00

  • Pharmacometabonomics and personalized medicine.

    abstract:BACKGROUND:Pharmacometabonomics is a new branch of science, first described in 2006 and defined as 'the prediction of the effects of a drug on the basis of a mathematical model of pre-dose metabolite profiles'. Pharmacometabonomics has been used to predict drug metabolism, pharmacokinetics (PK), drug safety and drug ef...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1177/0004563213497929

    authors: Everett JR,Loo RL,Pullen FS

    更新日期:2013-11-01 00:00:00

  • Long-term studies on immunoreactive human melatonin.

    abstract::Immunoreactive melatonin has been studied in normal human volunteers, both men and women, at different times of day and different times of the year. At 2400, but not at 0800 or 1200, there are very large inter-individual variations in mean levels, the maximum difference being fivefold. Intra-individual variations are ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456327901600182

    authors: Arendt J,Wirz-Justice A,Bradtke J,Kornemark M

    更新日期:1979-11-01 00:00:00

  • Immunoturbidimetric measurement of serum retinol-binding protein in renal and hepatic disease.

    abstract::Serum retinol-binding protein (RBP), with a biological half-life of less than 12 h, is a useful indicator of liver or kidney dysfunction. An automated immunoturbidimetric assay for the measurement of RBP has been developed using the Cobas-BIO centrifugal analyser and commercially available materials. Serum samples wit...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328802500309

    authors: Bankson DD,Rifai N,Silverman LM

    更新日期:1988-05-01 00:00:00

  • Identification of an unusual gamma counter fault using Trigg's technique for trend detection.

    abstract::A simultaneous deterioration in performance of several radioimmunoassays suggested that equipment was malfunctioning. Replicate counts from an automated gamma counter showed unacceptable variation during a 24-hour period; this variation was detected using Trigg's technique, a sensitive method for trend analysis, and w...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456327801500126

    authors: Challand GS,Cox L

    更新日期:1978-03-01 00:00:00

  • Enhanced luminescent quantitation of horseradish peroxidase conjugates: application in an enzyme immunoassay for digoxin.

    abstract::A p-iodophenol-enhanced luminescent end-point has been incorporated into a commercially available heterogeneous competitive enzyme immunoassay for digoxin based on a horseradish peroxidase-digoxin conjugate. The luminescent end-point could be completed in less than 1 min and significantly reduced overall assay time. R...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328602300104

    authors: Coyle PM,Thorpe GH,Kricka LJ,Whitehead TP

    更新日期:1986-01-01 00:00:00

  • Interference in immunoassay is an underestimated problem.

    abstract::The presence of antibodies in some patients' serum has long been known to be a potential source of interference in immunoassays, as shown by numerous case reports. These often appear after the introduction of a new analyte (e.g. troponin) and then decrease in number as the topic becomes exhausted. This highlights the ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1258/000456302760042128

    authors: Ismail AA,Walker PL,Cawood ML,Barth JH

    更新日期:2002-07-01 00:00:00

  • Effect of ambient temperature on analytical and clinical performance of a blood glucose monitoring system: Omnitest Sensor glucose meter.

    abstract::The effect of ambient temperature on the analytical and clinical performance of a glucose meter was examined. A total of 114 venous whole blood samples were analysed for glucose by a reference method, and by a glucose meter at 21-22 degrees C, room temperatures, 26-27 degrees C and 33-34 degrees C. Glucose meter readi...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/0004563011901091

    authors: Nawawi H,Sazali BS,Kamaruzaman BH,Yazid TN,Jemain AA,Ismail F,Khalid BA

    更新日期:2001-11-01 00:00:00

  • Antigen binding in the two-site immunoradiometric assay for serum ferritin: the nature of the hook effect.

    abstract::The two stages of a two-site immunoradiometric assay were investigated separately. In the first stage, the amount of ferritin bound to coated tubes initially showed a rapid increase with increasing concentration of added ferritin. This was followed by a plateau and then a further increase which appeared to be largely ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328402100510

    authors: Perera P,Worwood M

    更新日期:1984-09-01 00:00:00

  • The significance of serum troponin T in patients with kidney disease: a review of the literature.

    abstract::The last 10 years have witnessed radical changes in the definition and diagnosis of the acute coronary syndrome (ACS), including myocardial infarction, as a result of the introduction of sensitive and specific markers of myocardial damage, the cardiac troponins. One barrier to the universal acceptance of these markers...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1258/000456304322664645

    authors: Lamb EJ,Webb MC,Abbas NA

    更新日期:2004-01-01 00:00:00

  • A rapid method for measuring serum amyloid A protein by latex agglutination nephelometric immunoassay.

    abstract::Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enric...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329303000112

    authors: Yamada T,Nomata Y,Sugita O,Okada M

    更新日期:1993-01-01 00:00:00

  • Association of angiopoietin-like protein 3 with hepatic triglyceride lipase and lipoprotein lipase activities in human plasma.

    abstract:BACKGROUND:The relationship between plasma angiopoietin-like protein 3 (ANGPTL3), and lipoprotein lipase (LPL) activity and hepatic triglyceride lipase (HTGL) activity has not been investigated in the metabolism of remnant lipoproteins (RLPs) and high-density lipoprotein (HDL) in human plasma. METHODS:ANGPTL3, LPL act...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/acb.2010.009307

    authors: Nakajima K,Kobayashi J,Mabuchi H,Nakano T,Tokita Y,Nagamine T,Imamura S,Ai M,Otokozawa S,Schaefer EF

    更新日期:2010-09-01 00:00:00

  • Rapid enzymatic method for the measurement of mannitol in urine.

    abstract::A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or su...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329102800416

    authors: Blood J,Ingle AR,Allison N,Davies GR,Hill PG

    更新日期:1991-07-01 00:00:00

  • Can we interpret serum thyroglobulin results?

    abstract::Thyroglobulin (Tg) is a tumour marker for differentiated thyroid cancer. Interpretation requires a knowledge of the current thyrotropin (TSH) concentration as secretion is TSH-dependent. While a raised serum Tg may be indicative of residual or recurrent thyroid cancer, trauma to the thyroid (e.g. surgical, biopsy or d...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1258/acb.2011.011248

    authors: Clark P,Franklyn J

    更新日期:2012-07-01 00:00:00

  • Natural selection? The evolution of diagnostic criteria for gestational diabetes.

    abstract::Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1177/0004563216674743

    authors: Meek CL

    更新日期:2017-01-01 00:00:00

  • Mind the gap: shortcomings of the osmotic gap and a possible solution.

    abstract::Background Measured (MO) and calculated osmotic concentrations (CO) and the osmotic gap (OG) are commonly used in the investigation of electrolyte and volume disturbances as well as in cases of suspected volatile ingestion. Methods We compared 38 published formulae for CO with MO on a large data-set ( n = 9466) and ad...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563217694052

    authors: Wilgen U,Pretorius CJ,Ungerer JP

    更新日期:2018-01-01 00:00:00

  • Investigation of the use of bilirubin oxidase to measure the apparent unbound bilirubin concentration in human plasma.

    abstract::An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328802500110

    authors: Goldfinch ME,Maguire GA

    更新日期:1988-01-01 00:00:00

  • Glucose interference in the pancreolauryl test.

    abstract::The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329803500212

    authors: Trewick AL

    更新日期:1998-03-01 00:00:00

  • Prognostic evaluation of serum long non-coding RNA H19 for endoscopic keyhole surgery or craniotomy in glioma.

    abstract:BACKGROUND:Our study aims to explore the effect of serum long non-coding RNA (lncRNA) H19 level on the long-term prognosis of endoscopic keyhole surgery or craniotomy for glioma. METHODS:A total of 264 glioma patients were selected. Patients were randomly divided into the Craniotomy-high H19 group, the Craniotomy-low ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563220941888

    authors: Shen L,Xu M,Wang Z,Yu Z

    更新日期:2020-09-01 00:00:00

  • Serum total sialic acid and lipid-associated sialic acid in normal individuals and patients with myocardial infarction, and their relationship to acute phase proteins.

    abstract::Serum total sialic acid (TSA) has gained medical interest, particularly as a cardiovascular risk factor and it has been hypothesized that serum levels relate to serum acute phase proteins, some of which are sialylated. We assayed serum TSA and also lipid associated sialic acid (LASA) in 50 normal individuals (24 male)...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329303000406

    authors: Haq M,Haq S,Tutt P,Crook M

    更新日期:1993-07-01 00:00:00