Serum digoxin assays: investigation of use and abuse.

abstract：:Homocysteine, a sulphur amino acid, is a branch-point intermediate of methionine metabolism. It can be degraded in the transsulphuration pathway to cystathionine, or remethylated to methionine via the remethylation pathway. In both pathways, major genetic defects that cause enzyme deficiencies are associated with very...

journal_title：Annals of clinical biochemistry

authors： Lievers KJ,Kluijtmans LA,Blom HJ

更新日期：2003-01-01 00:00:00

abstract：:The deproteinised sample (150 microliters) is 'injected' into a continuously flowing stream of deionised water which is pumped, via a capillary tube, to the nebuliser of an atomic absorption spectrophotometer. Analytical readout is obtained, in the form of transient peaks, 6 s after sample injection. Before injection ...

journal_title：Annals of clinical biochemistry

authors： Rocks BF,Sherwood RA,Turner ZJ,Riley C

更新日期：1983-03-01 00:00:00

abstract：:During incubation with rabbit blood in vitro rabbit-muscle lactate dehydrogenase-5 was inactivated at a rate similar to that observed in vivo. By contrast plasma and plasma containing erythrocytes had no effect on the enzyme activity, but plasma containing leucocytes inactivated the enzyme at the same rate as whole bl...

journal_title：Annals of clinical biochemistry

authors： Qureshi AR,Wilkinson JH

更新日期：1977-01-01 00:00:00

abstract：:A patient with sepsis and jaundice was admitted for diagnosis and treatment. Associated biochemical changes included increased C-reactive protein, conjugated bilirubin and gamma-glutamyltransferase, the duration of which was protracted. High urine coproporphyrin isomer-1 and immunostaining of liver tissue suggested Du...

journal_title：Annals of clinical biochemistry

authors： Devgun MS,El-Nujumi AM,O'Dowd GJ,Barbu V,Poupon R

更新日期：2012-11-01 00:00:00

abstract：:The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed fo...

journal_title：Annals of clinical biochemistry

authors： Starcher B,Scott M

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:The stability of parathyroid hormone (PTH) in whole blood has not been well characterized. Previous studies show EDTA plasma samples to be more stable than serum samples. METHODS:Blood from 17 haemodialysis patients and five healthy volunteers was collected into serum separator tubes (SSTs) and potassium ED...

journal_title：Annals of clinical biochemistry

authors： English E,McFarlane I,Taylor KP,Halsall DJ

更新日期：2007-05-01 00:00:00

abstract：:Guaiac-based faecal occult blood tests (gFOBT) are still used in asymptomatic population bowel screening programmes but are being replaced by faecal immunochemical tests (FIT) for haemoglobin. gFOBT have many well-documented disadvantages and there is little evidence for their use in assessment of the symptomatic. Man...

journal_title：Annals of clinical biochemistry

authors： Fraser CG

更新日期：2012-11-01 00:00:00

abstract：:A group of international experts prepared two lists of drugs with their serum/plasma and urine concentrations, which should be used when evaluating the performance of a new laboratory method. The two lists were verified by running in vitro interference studies in three European laboratories on Hitachi instruments. The...

journal_title：Annals of clinical biochemistry

authors： Sonntag O,Scholer A

更新日期：2001-07-01 00:00:00

abstract：:Eccentric exercise causes release of muscle creatine kinase (CK) 3-4 days after exercise. Racial variation in basal serum CK has been reported but the reasons for this are unknown. We studied 30 subjects of different ethnic origin (Caucasian, Afro-Caribbean, Asian) before and after eccentric exercise. Basal serum CK w...

journal_title：Annals of clinical biochemistry

authors： Sherwood RA,Lambert A,Newham DJ,Wassif WS,Peters TJ

更新日期：1996-07-01 00:00:00

abstract：:The Poser criteria for diagnosing multiple sclerosis (MS) includes clinical, paraclinical and laboratory information. We studied the influence of cerebrospinal fluid (CSF) biochemistry results on the categorisation of patients with suspected MS. A retrospective study was made of 138 patients who had CSF samples sent o...

journal_title：Annals of clinical biochemistry

authors： Ratnaike S,Kilpatrick T,Tress B,Davis S,Kilpatrick C,Byron K,Deam D

更新日期：1990-05-01 00:00:00

abstract：BACKGROUND AND METHODS:We report a case of insulinoma in which the diagnosis was very challenging as some of the biochemical data were consistently equivocal. In order to assess the relative reliability of the analytical tests, retrospective biochemical data on 45 other cases of histologically confirmed insulinoma were...

journal_title：Annals of clinical biochemistry

authors： Chammas NK,Teale JD,Quin JD

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:It is important to explain target proteins for the understanding of the pathogenesis of vitreoretinal diseases. In a previous study, we identified more than 100 proteins including seven angiogenic-modulated factors in vitreous humours (VHs). Although there have been many reports of expressed protein profiles...

journal_title：Annals of clinical biochemistry

authors： Mukai N,Nakanishi T,Shimizu A,Takubo T,Ikeda T

更新日期：2008-05-01 00:00:00

abstract：:The diagnostic usefulness of fasting total serum bile acids (SBA/F) in the detection of liver diseases and assessment of different aspects of hepatic function alteration was evaluated in 61 healthy subjects and 186 patients with liver disease. The value of SBA/F was compared with other routine tests. In 49 healthy sub...

journal_title：Annals of clinical biochemistry

authors： Cravetto C,Molino G,Biondi AM,Cavanna A,Avagnina P,Frediani S

更新日期：1985-11-01 00:00:00

abstract：BACKGROUND:Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipept...

journal_title：Annals of clinical biochemistry

authors： Ghodsi R,Kheirouri S,Nosrati R

更新日期：2019-01-01 00:00:00

abstract：:The measurement of glycated haemoglobin (glycated Hb) by automated high pressure liquid chromatography enabled the prevalence of haemoglobin variants (Hb variant) to be determined in a large diabetic population within Leeds, UK. There were 16 patients showing a Hb variant amongst a total of 5300 diabetics, the majorit...

journal_title：Annals of clinical biochemistry

authors： Allen KR,Hamilton AD,Bodansky HJ,Poon P

更新日期：1992-07-01 00:00:00

abstract：:Using a high-performance liquid chromatography method, we measured seven commonly prescribed benzodiazepines (chlordiazepoxide, nitrazepam, nordiazepam, oxazepam, lorazepam, temazepam and diazepam) in 100 urine samples obtained from patients attending the Leeds Addiction Unit. All of the urines selected for investigat...

journal_title：Annals of clinical biochemistry

authors： Garretty DJ,Wolff K,Hay AW,Raistrick D

更新日期：1997-01-01 00:00:00

abstract：:We developed a binary gradient high-performance liquid chromatography (HPLC) method for measuring HbA2 in whole blood samples using a Pharmacia Mono Q column (1 mL) and measurement at 415 nm. The assay requires a simple lysis and centrifugation step before injection onto the column. We found good agreement of results ...

journal_title：Annals of clinical biochemistry

authors： Keevil BG,Maylor PW,Rowlands D

更新日期：1996-05-01 00:00:00

abstract：:A rapid and simple method for detecting circulating immune complexes based on turbidity measurements following polyethylene glycol precipitation was studied with regard to its suitability as a routine assay in clinical laboratories. This method was found to have an acceptable degree of precision provided the temperatu...

journal_title：Annals of clinical biochemistry

authors： Kilpatrick DC,Weston J,Irvine WJ

更新日期：1981-11-01 00:00:00

abstract：:The aim of this study was to assess the performance of a commercially available procedure for detecting anti-Sjögren's syndrome A (anti-SSA) and anti-Sjögren's syndrome B (anti-SSB) antibodies by immunoblotting (IB) and compare it with double immunodiffusion (DID). We also studied the clinical significance of these pr...

journal_title：Annals of clinical biochemistry

authors： Yago M,Belmonte MA,Olmos MJ,Beltran J,Teruel C,Segarra M

更新日期：1999-05-01 00:00:00

abstract：:γ-glutamyltransferase plays a key role in the synthesis and metabolism of extracellular glutathione, a major antioxidant in several defence mechanisms in the body. γ-glutamyltransferase is affected by environmental and genetic factors, and is raised when there is depletion of glutathione. Hence, it is a marker of oxid...

journal_title：Annals of clinical biochemistry

authors： Bulusu S,Sharma M

更新日期：2016-05-01 00:00:00

abstract：:Plasma C1 inhibitor (C1-INH) was determined in 64 patients with different malignant diseases and in 58 healthy persons. C1-INH antigen concentration was measured by radial immunodiffusion (RID), whereas its functional activity was assayed with chromogenic substrate. Within-run and day-to-day precision of both methods ...

journal_title：Annals of clinical biochemistry

authors： Starcević D,Jelić-Ivanović Z,Kalimanovska V

更新日期：1991-11-01 00:00:00

abstract：BACKGROUND:It is now established that the bioactive lipid lysophosphatidic acid (LPA) contributes to cancer initiation, progression and metastasis, including those of prostate cancer. LPA is produced in the serum and plasma mainly by conversion from lysophospholipids through the action of lysophospholipase D (lysoPLD),...

journal_title：Annals of clinical biochemistry

authors： Nakamura K,Takeuchi T,Ohkawa R,Okubo S,Yokota H,Tozuka M,Aoki J,Arai H,Ikeda H,Ohshima N,Kitamura T,Yatomi Y

更新日期：2007-11-01 00:00:00

abstract：:Four hundred and fifty-four subjects with hyperamylasaemia were screened for the presence of macroamylase using polyethylene glycol precipitation (PEG) and the Beckman automated amylase assay based on the hydrolysis of maltotetraose. Twenty-five subjects (5.5%) exhibited PEG precipitation values suggestive of macroamy...

journal_title：Annals of clinical biochemistry

authors： Lawson GJ

更新日期：2001-01-01 00:00:00

abstract：:The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...

journal_title：Annals of clinical biochemistry

authors： Crook MA,Velauthar U,Moran L,Griffiths W

更新日期：1999-03-01 00:00:00

abstract：BACKGROUND:Our study aims to explore the effect of serum long non-coding RNA (lncRNA) H19 level on the long-term prognosis of endoscopic keyhole surgery or craniotomy for glioma. METHODS:A total of 264 glioma patients were selected. Patients were randomly divided into the Craniotomy-high H19 group, the Craniotomy-low ...

journal_title：Annals of clinical biochemistry

authors： Shen L,Xu M,Wang Z,Yu Z

更新日期：2020-09-01 00:00:00

abstract：:A case is presented of a 41-year-old man with a history of recurrent renal stones over 10 years. Analysis of the stone showed that, although it gave a positive reaction with the non-specific phosphotungstic acid test, uricase failed to identify any urate present. Analysis in a reference laboratory confirmed its compos...

journal_title：Annals of clinical biochemistry

authors： Wilkinson H,Samuell C,Stower M

更新日期：2004-03-01 00:00:00

abstract：:A method for the determination of metanephrine (MN; also known as metadrenaline), normetanephrine (NMN; also known as normetadrenaline) and 3-methoxytyramine (3-MT) in human urine using high-performance liquid chromatography followed by electrochemical detection (ECD) was validated primarily by comparing the results w...

journal_title：Annals of clinical biochemistry

authors： Willemsen JJ,Ross HA,Wolthers BG,Sweep CG,Kema IP

更新日期：2001-11-01 00:00:00

abstract：:Background Antiphospholipid syndrome is an autoimmune disease characterized by thrombosis, fetal losses and thrombocytopenia associated to antiphospholipid antibodies. They are directed to phospholipids, such as cardiolipins (anticardiolipin) and lupus anticoagulant or to complexes formed by phospholipids and protein ...

journal_title：Annals of clinical biochemistry

authors： Pelusa HF,Pezzarini E,Basiglio CL,Musuruana J,Bearzotti M,Svetaz MJ,Daniele SM,Bottai H,Arriaga SM

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:Measurement of plasma-free metanephrine plus normetanephrine (PFM) is the best screening test for phaeochromocytoma. While clearly raised levels are diagnostic, borderline increases may be associated with factors such as stress and medications, and should prompt a repeat study after interfering factors are w...

journal_title：Annals of clinical biochemistry

authors： Anas SS,Vasikaran SD

更新日期：2010-11-01 00:00:00

abstract：:Some inherited metabolic disorders (IMDs) can cause significant complications during pregnancy, affecting the mother and/or the fetus. Although it appears that only a minority of IMDs have these effects, experience is still being acquired. For some disorders, patients will not have reached child-bearing age. Pregnanci...

journal_title：Annals of clinical biochemistry

authors： Preece MA,Green A

更新日期：2002-09-01 00:00:00