Abstract:
BACKGROUND:The stability of parathyroid hormone (PTH) in whole blood has not been well characterized. Previous studies show EDTA plasma samples to be more stable than serum samples. METHODS:Blood from 17 haemodialysis patients and five healthy volunteers was collected into serum separator tubes (SSTs) and potassium EDTA tubes (EDTA-Ts) and incubated at room temperature before separation and storage for PTH analysis using the Bayer Advia Centaur. RESULTS:Initial PTH concentrations were 7% higher (95% confidence interval [CI] 2-16%, P=0.016) in EDTA-Ts compared to the SSTs in the renal cohort. PTH concentrations in SSTs were significantly lower after 8 (10%, 95% CI 3-17%) and 20 h (22%, 95% CI 11-31%) but did not change significantly in EDTA-Ts (P=0.1543). PTH concentration in EDTA-Ts from healthy volunteers was also independent of incubation time (P=0.1165). CONCLUSION:The greater stability of PTH in whole blood anticoagulated with potassium EDTA allows PTH analysis to be offered to sites such as satellite clinics and primary care sites which do not have centrifugation and refrigeration facilities.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
English E,McFarlane I,Taylor KP,Halsall DJdoi
10.1258/000456307780480927subject
Has Abstractpub_date
2007-05-01 00:00:00pages
297-9issue
Pt 3eissn
0004-5632issn
1758-1001journal_volume
44pub_type
杂志文章abstract:BACKGROUND:Calculated globulin fraction is derived from the liver function tests by subtracting albumin from the total protein. Since immunoglobulins comprise the largest component of the serum globulin concentration, increased or decreased calculated globulins and may identify patients with hypogammaglobulinaemia or h...
journal_title:Annals of clinical biochemistry
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abstract::We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are...
journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
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journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456328702400604
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abstract::Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destr...
journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
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doi:10.1177/000456329303000108
更新日期:1993-01-01 00:00:00
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journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500313
更新日期:1998-05-01 00:00:00
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journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456328702400401
更新日期:1987-07-01 00:00:00
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journal_title:Annals of clinical biochemistry
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doi:10.1258/acb.2010.009169
更新日期:2010-05-01 00:00:00
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journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563041731583
更新日期:2004-09-01 00:00:00
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journal_title:Annals of clinical biochemistry
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doi:10.1177/000456329803500117
更新日期:1998-01-01 00:00:00
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journal_title:Annals of clinical biochemistry
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journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Annals of clinical biochemistry
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更新日期:2004-03-01 00:00:00
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journal_title:Annals of clinical biochemistry
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更新日期:2015-09-01 00:00:00
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journal_title:Annals of clinical biochemistry
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更新日期:2015-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2004-07-01 00:00:00