Interference of ascorbic acid with chemical analytes.

Abstract:

BACKGROUND:Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20. METHODS:Various concentrations of ascorbic acid were added to serum, and the serum analytes were measured on the LX20. RESULTS:With a serum ascorbic acid concentration of 12.0 mmol/L, the values for sodium, potassium, calcium and creatinine increased by 43%, 58%, 103% and 26%, respectively (P<0.01). With a serum ascorbic acid concentration of 12.0 mmol/L, the values for chloride, total bilirubin and uric acid decreased by 33%, 62% and 83%, respectively (P<0.01), and were undetectable for total cholesterol, triglyceride, ammonia and lactate. There was no definite influence of ascorbic acid on analytical values for total CO(2), urea, glucose, phosphate, total protein, albumin, amylase, creatine kinase, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total iron, unbound iron-binding capacity or magnesium. CONCLUSIONS:Ascorbic acid causes a false increase in sodium, potassium, calcium and creatinine results and a false decrease in chloride, total bilirubin, uric acid, total cholesterol, triglyceride, ammonia and lactate results.

journal_name

Ann Clin Biochem

authors

Meng QH,Irwin WC,Fesser J,Massey KL

doi

10.1258/000456305774538274

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

475-7

issue

Pt 6

eissn

0004-5632

issn

1758-1001

journal_volume

42

pub_type

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