31P NMR spectroscopy of 9L cell line in culture: differential effects of high temperature on anchored cells and spheroids.

Abstract:

:The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45 degrees C for anchored cells and 43 degrees C for multicellular spheroids, respectively. Phosphomonoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern.

journal_name

Magn Reson Med

authors

Suzuki N,Kwee IL,Tamas LB,Nakada T

doi

10.1002/mrm.1910190233

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

422-8

issue

2

eissn

0740-3194

issn

1522-2594

journal_volume

19

pub_type

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