Unambiguous assignment of the H3S and H3R deuterations of cerebral (2-13C) glutamate by 13C NMR at 18.8 Tesla.

Abstract:

:We used high-field (13)C NMR (18.8 T) to assign unambiguously the isotopic shifts induced by the deuterium substitutions of the H3(proR) and H3(proS) hydrogens of (2-(13)C) glutamate in extracts of the brain from deuterated animals. Monodeuterated H3R or H3S glutamate diastereoisomers were produced stereospecifically either by chemical synthesis or by coupling the reactions of isocitrate dehydrogenase and aspartate aminotransferase in deuterated medium, respectively. We show that the (3S-(2)H) or (3R-(2)H) deuterations induce characteristic small (Delta(2) = -0.058 parts per million (ppm)) or large (Delta(2) = -0.071 ppm) vicinal isotopic shifts upfield of the perprotonated (2-(13)C) glutamate resonance (at 55.5 ppm). Isotopically shifted (2-(13)C, 3S-(2)H) or (2-(13)C, 3R-(2)H) glutamate singlets are conveniently observed by high-field (13)C NMR in brain extracts from deuterated rats. Since the (3S-(2)H) or (3R-(2)H) glutamate diastereoisomers are produced stereospecifically by the cytosolic or mitochondrial isoforms of aconitase and isocitrate dehydrogenase, our results will facilitate the (13)C NMR investigation of these enzymatic activities and their role in subcellular glutamate trafficking.

journal_name

Magn Reson Med

authors

Rodrigues TB,Violante IR,Cerdán S

doi

10.1002/mrm.22277

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

1088-91

issue

4

eissn

0740-3194

issn

1522-2594

journal_volume

63

pub_type

杂志文章
  • Water and fat MR imaging with chemical shift selective 3D steady state methods.

    abstract::A new 3D acquisition regimen that enables the collection of conventional, water-suppressed, and fat-suppressed images with no increase in scan times compared with currently implemented 3D sequences is presented. The method is based on conventional 3D steady state with interleaved selective excitation of the fat resona...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910310403

    authors: Sun L,Aletras AH,Schmalbrock P,Skinner TE,Chakeres D,Irsik R,Robitaille PM

    更新日期:1994-04-01 00:00:00

  • One-minute whole-brain magnetization transfer ratio imaging with intrinsic B1-correction.

    abstract:PURPOSE:Magnetization transfer ratio (MTR) histograms are used widely for the assessment of diffuse pathological changes in the brain. For broad clinical application, MTR scans should not only be fast, but confounding factors should also be minimized for high reproducibility. To this end, a 1-minute whole-brain spiral ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28618

    authors: Afshari R,Santini F,Heule R,Meyer CH,Pfeuffer J,Bieri O

    更新日期:2020-12-08 00:00:00

  • SLIP, a lipid suppression technique to improve image contrast in inflow angiography.

    abstract::In rapidly acquired gradient echo images, lipid regions usually appear as areas of high signal intensity. In 2D inflow angiograms processed by the maximum intensity projection algorithm, this high lipid signal may prevent angiographic visualization of some blood vessels. We present a new approach to suppress the lipid...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910210110

    authors: Doyle M,Matsuda T,Pohost GM

    更新日期:1991-09-01 00:00:00

  • Accelerated cardiac magnetic resonance imaging in the mouse using an eight-channel array at 9.4 Tesla.

    abstract::MRI has become an important tool to noninvasively assess global and regional cardiac function, infarct size, or myocardial blood flow in surgically or genetically modified mouse models of human heart disease. Constraints on scan time due to sensitivity to general anesthesia in hemodynamically compromised mice frequent...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22605

    authors: Schneider JE,Lanz T,Barnes H,Stork LA,Bohl S,Lygate CA,Ordidge RJ,Neubauer S

    更新日期:2011-01-01 00:00:00

  • Orientational dependence of T2 relaxation in articular cartilage: A microscopic MRI (microMRI) study.

    abstract::The experiments reported herein are the first MRI investigations of the orientational dependence of T(2) relaxation in articular cartilage at microscopic resolution over the 360 degrees angular space. For each of six canine cartilage specimens, 48 independent T(2)-weighted proton images were acquired for 12 different ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10216

    authors: Xia Y,Moody JB,Alhadlaq H

    更新日期:2002-09-01 00:00:00

  • Polymer thick film technology for improved simultaneous dEEG/MRI recording: Safety and MRI data quality.

    abstract:PURPOSE:To develop a 256-channel dense-array electroencephalography (dEEG) sensor net (the Ink-Net) using high-resistance polymer thick film (PTF) technology to improve safety and data quality during simultaneous dEEG/MRI. METHODS:Heating safety was assessed with temperature measurements in an anthropomorphic head pha...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26116

    authors: Poulsen C,Wakeman DG,Atefi SR,Luu P,Konyn A,Bonmassar G

    更新日期:2017-02-01 00:00:00

  • fMRI of auditory stimulation with intermolecular double-quantum coherences (iDQCs) at 1.5T.

    abstract::An intermolecular double-quantum coherence (iDQC) imaging technique was used to study auditory activation in the human brain at 1.5T with a dual temporal lobe surface phased array coil and a quadrature head coil. Preliminary results demonstrate that it is feasible to obtain auditory activation maps using iDQC imaging ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/1522-2594(200103)45:3<356::aid-mrm1046>3.0

    authors: Zhong J,Kwok E,Chen Z

    更新日期:2001-03-01 00:00:00

  • A comparison of static and dynamic ∆B0 mapping methods for correction of CEST MRI in the presence of temporal B0 field variations.

    abstract:PURPOSE:To assess the performance, in the presence of scanner instabilities, of three dynamic correction methods which integrate ∆B0 mapping into the chemical exchange saturation transfer (CEST) measurement and three established static ∆B0 -correction approaches. METHODS:A homogeneous phantom and five healthy voluntee...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27750

    authors: Poblador Rodriguez E,Moser P,Dymerska B,Robinson S,Schmitt B,van der Kouwe A,Gruber S,Trattnig S,Bogner W

    更新日期:2019-08-01 00:00:00

  • 3D-printed Shepp-Logan phantom as a real-world benchmark for MRI.

    abstract:PURPOSE:As prescribed and reliable geometrical entities, phantoms have served as indispensable validation tools in a variety of MR-related topics. Though a number of phantoms have been conceived, certain applications may warrant highly customized geometries. The purpose of this study was to demonstrate the expediency o...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.25593

    authors: Kasten JA,Vetterli T,Lazeyras F,Van De Ville D

    更新日期:2016-01-01 00:00:00

  • 4D radial coronary artery imaging within a single breath-hold: cine angiography with phase-sensitive fat suppression (CAPS).

    abstract::Coronary artery data acquisition with steady-state free precession (SSFP) is typically performed in a single frame in mid-diastole with a spectrally selective pulse to suppress epicardial fat signal. Data are acquired while the signal approaches steady state, which may lead to artifacts from the SSFP transient respons...

    journal_title:Magnetic resonance in medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/mrm.20627

    authors: Park J,Larson AC,Zhang Q,Simonetti O,Li D

    更新日期:2005-10-01 00:00:00

  • Mapping of redox status in a brain-disease mouse model by three-dimensional EPR imaging.

    abstract::Electron paramagnetic resonance imaging using nitroxides is a powerful method for visualizing the redox status modulated by oxidative stress in vivo. Typically, however, data acquisition times have been too slow to obtain a sufficient number of projections for three-dimensional images, when using continuous wave-elect...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22598

    authors: Fujii H,Sato-Akaba H,Kawanishi K,Hirata H

    更新日期:2011-01-01 00:00:00

  • A reduced field-of-view method to increase temporal resolution or reduce scan time in cine MRI.

    abstract::In some dynamic applications of MRI, only a part of the field-of-view (FOV) actually undergoes dynamic changes. A class of methods, called reduced-FOV (rFOV) methods, convert the knowledge that some part of the FOV is static or not very dynamic into an increase in temporal resolution for the dynamic part, or into a re...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1522-2594(200004)43:4<549::aid-mrm9>

    authors: Madore B,Fredrickson JO,Alley MT,Pelc NJ

    更新日期:2000-04-01 00:00:00

  • Capillarization of the sinusoids in liver fibrosis: noninvasive assessment with contrast-enhanced MRI in the rabbit.

    abstract::Sinusoidal capillarization induces microcirculatory changes in liver cirrhosis and fibrosis. The purpose of this study was to assess whether contrast-enhanced MRI can be used to demonstrate the effects of sinusoidal capillarization in liver fibrosis. Dynamic MRI after injection of a low-molecular-weight contrast agent...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10420

    authors: Van Beers BE,Materne R,Annet L,Hermoye L,Sempoux C,Peeters F,Smith AM,Jamart J,Horsmans Y

    更新日期:2003-04-01 00:00:00

  • Localized two-dimensional shift correlated spectroscopy in humans at 2 Tesla.

    abstract::A method for the acquisition of localized 2D shift-correlated spectra, based on the combination of the stimulated-echo volume-selection and gradient-enhanced COSY experiments, is described. The sequence can be modified to perform a number of localized experiments including HOHAHA and DQF-COSY. The method is demonstrat...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910320216

    authors: Brereton IM,Galloway GJ,Rose SE,Doddrell DM

    更新日期:1994-08-01 00:00:00

  • Efficient sample density estimation by combining gridding and an optimized kernel.

    abstract::The reconstruction of non-Cartesian k-space trajectories often requires the estimation of nonuniform sampling density. Particularly for 3D, this calculation can be computationally expensive. The method proposed in this work combines an iterative algorithm previously proposed by Pipe and Menon (Magn Reson Med 1999;41:1...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.23041

    authors: Zwart NR,Johnson KO,Pipe JG

    更新日期:2012-03-01 00:00:00

  • Manganese-enhanced MRI of salivary glands and head and neck tumors in living subjects.

    abstract::Manganese-enhanced MRI has previously been used for visualization of brain architecture and functional mapping of neural pathways. The present work investigated the potential of manganese-enhanced MRI for noninvasive imaging of salivary glands in living subjects. Marked shortening of T(1) was observed in salivary glan...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22452

    authors: Seshadri M,Hoy A

    更新日期:2010-09-01 00:00:00

  • Accelerated interleaved spiral-IDEAL imaging of hyperpolarized 129 Xe for parametric gas exchange mapping in humans.

    abstract:PURPOSE:To demonstrate the feasibility of mapping gas exchange with single breath-hold hyperpolarized (HP) 129 Xe in humans, acquiring parametric maps of lung physiology. The potential benefit of acceleration using parallel imaging for this application is also explored. METHODS:Six healthy volunteers were scanned with...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27765

    authors: Zanette B,Santyr G

    更新日期:2019-09-01 00:00:00

  • T1 measurements in the human myocardium: the effects of magnetization transfer on the SASHA and MOLLI sequences.

    abstract:PURPOSE:Quantitative mapping of the native T1 of the heart using the modified look-locker inversion recovery (MOLLI) technique provides high quality diagnostic information without requiring contrast agents. Previous work has considered the effects of T2 relaxation on MOLLI T1 measurements, finding that the T1 measured ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24867

    authors: Robson MD,Piechnik SK,Tunnicliffe EM,Neubauer S

    更新日期:2013-09-01 00:00:00

  • Fast nonlinear susceptibility inversion with variational regularization.

    abstract:PURPOSE:Quantitative susceptibility mapping can be performed through the minimization of a function consisting of data fidelity and regularization terms. For data consistency, a Gaussian-phase noise distribution is often assumed, which breaks down when the signal-to-noise ratio is low. A previously proposed alternative...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27073

    authors: Milovic C,Bilgic B,Zhao B,Acosta-Cabronero J,Tejos C

    更新日期:2018-08-01 00:00:00

  • Localized 1H NMR measurements of 2-pyrrolidinone in human brain in vivo.

    abstract::Localized 1H NMR homonuclear J editing spectroscopy was used to measure the concentration of 2-pyrrolidinone (PRDN) in the human occipital lobe of five normal and six epileptic subjects taking vigabatrin. PRDN is a lactam cyclization product of gamma-aminobutyric acid (GABA). From a localized volume of 13.5 cm3 in the...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1522-2594(199905)41:5<889::aid-mrm6>

    authors: Hyder F,Petroff OA,Mattson RH,Rothman DL

    更新日期:1999-05-01 00:00:00

  • Global and peak local specific absorption rate control on parallel transmit systems using k-means SAR compression model.

    abstract:PURPOSE:To improve the specific absorption rate (SAR) compression model capability in parallel transmission (pTx) MRI systems. METHODS:A k-means clustering method is proposed to group voxels with similar SAR behaviors in the scanned object, providing a controlled upper-bounded estimation of peak local SARs. This k-mea...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28456

    authors: Mao X,Bari S,Love DJ,Rispoli JV

    更新日期:2021-02-01 00:00:00

  • Water suppression with B0 field gradient homospoil pulses in high-resolution NMR spectroscopy.

    abstract::The combination of a frequency nonselective excitation suppression method (1331 sequence) with selective excitation followed by gradient-induced dephasing of water transverse magnetization yielded suppression ratios of greater than 10,000:1. The need for gradient preemphasis and correction of B0 field shifts is discus...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910090114

    authors: Brereton IM,Field J,Moxon LN,Irving MG,Doddrell DM

    更新日期:1989-01-01 00:00:00

  • Perfusion-based event-related functional MRI.

    abstract::Perfusion-based event-related functional MRI was performed by measuring flow-sensitive alternating inversion recovery (FAIR) signal changes during repeated single-trial, short visual stimulation (250 msec). In the visual cortex activation area, the blood flow increases immediately after the stimulus, reaches the maxim...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1522-2594(199912)42:6<1011::aid-mrm3

    authors: Liu HL,Gao JH

    更新日期:1999-12-01 00:00:00

  • Total brain N-acetylaspartate concentration in normal, age-grouped females: quantitation with non-echo proton NMR spectroscopy.

    abstract::The intra-individual and inter-individual variations of the global N-acetylaspartate (NAA) concentration were measured in a cohort of five 42+/-5 year-old normal females. The total NAA signal from the whole head was obtained with non-localized non-echo proton spectroscopy (1H-MRS) and converted into absolute mole amou...

    journal_title:Magnetic resonance in medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/mrm.1910400506

    authors: Gonen O,Viswanathan AK,Catalaa I,Babb J,Udupa J,Grossman RI

    更新日期:1998-11-01 00:00:00

  • Design and evaluation of quantitative MRI phantoms to mimic the simultaneous presence of fat, iron, and fibrosis in the liver.

    abstract:PURPOSE:To design, construct, and evaluate quantitative MR phantoms that mimic MRI signals from the liver with simultaneous control of three parameters: proton-density fat fraction (PDFF), R 2 ∗ ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28452

    authors: Zhao R,Hamilton G,Brittain JH,Reeder SB,Hernando D

    更新日期:2021-02-01 00:00:00

  • Quantification of fluid changes in rat leg joints with adjuvant arthritis by a one-dimensional magnetic resonance imaging experiment.

    abstract::A simple and reliable method for quantifying the fluid content and characterizing the tissue T2 relaxation properties of animal extremities has been devised. This one-dimensional magnetic resonance imaging experiment, applied to the adjuvant arthritis model in rats, provides a useful, noninvasive monitor of the course...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910150208

    authors: Borah B,Szeverenyi NM

    更新日期:1990-08-01 00:00:00

  • Kinetics of avidin-induced clearance of biotinylated bimodal liposomes for improved MR molecular imaging.

    abstract::Dual labeled liposomes, carrying both paramagnetic and fluorescent lipids, were recently proposed as potent contrast agents for MR molecular imaging. These nanoparticles are coated with poly(ethylene glycol) (PEG) to increase their blood circulation half-life, which should allow extensive accumulation at the targeted ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21780

    authors: van Tilborg GA,Strijkers GJ,Pouget EM,Reutelingsperger CP,Sommerdijk NA,Nicolay K,Mulder WJ

    更新日期:2008-12-01 00:00:00

  • Interactive continuously moving table (iCMT) large field-of-view real-time MRI.

    abstract::Continuously moving table (CMT) MRI is a new method that is capable of generating 3D, seamless, large field-of-view (FOV) images by acquiring readouts along the patient superior-inferior axis as the subject is translated through the scanner. For applications that require artifact-free images, such as arterial-phase co...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20848

    authors: Sabati M,Lauzon ML,Mahallati H,Frayne R

    更新日期:2006-05-01 00:00:00

  • Multi-band MR fingerprinting (MRF) ASL imaging using artificial-neural-network trained with high-fidelity experimental data.

    abstract:PURPOSE:We aim to leverage the power of deep-learning with high-fidelity training data to improve the reliability and processing speed of hemodynamic mapping with MR fingerprinting (MRF) arterial spin labeling (ASL). METHODS:A total of 15 healthy subjects were studied on a 3T MRI. Each subject underwent 10 runs of a m...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28560

    authors: Fan H,Su P,Huang J,Liu P,Lu H

    更新日期:2021-04-01 00:00:00

  • A fuse for magnetic resonance imaging probes.

    abstract::In a research environment, a magnetic resonance imaging system's manufacturer-supplied protection against excessive rf power deposition in the sample is often compromised. This paper presents a widely applicable scheme, based on a commonly available fuse, that simply and effectively limits the average rf power into a ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910090213

    authors: Hedges LK

    更新日期:1989-02-01 00:00:00