Abstract:
:Slowly cycling cells are believed to play a critical role in tumor progression and metastatic dissemination. The goal of this study was to develop a method for in vivo detection of slowly cycling cells. To distinguish these cells from more rapidly proliferating cells that constitute the vast majority of cells in tumors, we used the well-known effect of label dilution due to division of cells with normal cycle and retention of contrast agent in slowly dividing cells. To detect slowly cycling cells, melanoma cells were labeled with iron oxide particles. After labeling, we observed dilution of contrast agent in parallel with cell proliferation in the vast majority of normally cycling cells. A small and distinct subpopulation of iron-retaining cells was detected by flow cytometry after 20 days of in vitro proliferation. These iron-retaining cells exhibited high expression of a biological marker of slowly cycling cells, JARID1B. After implantation of labeled cells as xenografts into immunocompromised mice, iron-retaining cells were detected in vivo and ex vivo by magnetic resonance imaging that was confirmed by Prussian Blue staining. Magnetic resonance imaging detects not only iron retaining melanoma cells but also iron positive macrophages. Proposed method opens up opportunities to image subpopulation of melanoma cells, which is critical for continuous tumor growth.
journal_name
Magn Reson Medjournal_title
Magnetic resonance in medicineauthors
Magnitsky S,Roesch A,Herlyn M,Glickson JDdoi
10.1002/mrm.22917subject
Has Abstractpub_date
2011-11-01 00:00:00pages
1362-73issue
5eissn
0740-3194issn
1522-2594journal_volume
66pub_type
杂志文章abstract:PURPOSE:Magnetic resonance imaging of humans at high magnetic field strengths is strongly influenced by the interference of the radiofrequency (RF) electromagnetic field and the body. To minimize this effect, multiple RF sources could be used. A novel setup (called multimode, coaxial waveguide) is proposed that facilit...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24807
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:We describe a 2 × 6 channel sodium/proton array for knee MRI at 3T. Multielement coil arrays are desirable because of well-known signal-to-noise ratio advantages over volume and single-element coils. However, low tissue-coil coupling that is characteristic of coils operating at low frequency can make the potent...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.26017
更新日期:2016-10-01 00:00:00
abstract::In this work, a new double quantum filter for glutathione (GSH) editing is introduced, combined with the point-resolved spectroscopy localization sequence (PRESS), and demonstrated in vivo. Compared to the conventional double quantum coherence filter, the new filter has two major advantages. First, it eliminates the n...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.20788
更新日期:2006-03-01 00:00:00
abstract::When using tracer kinetic modeling to analyze dynamic contrast-enhanced MRI (DCE-MRI) it is necessary to identify an appropriate arterial input function (AIF). The measured AIF is often poorly sampled in both clinical and preclinical MR systems due to the initial rapid increase in contrast agent concentration and the ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21959
更新日期:2009-05-01 00:00:00
abstract::Two single-shot volume imaging techniques are described. The first, single-echo echo-volume imaging, is essentially the echo-volume imaging (EVI) sequence suggested by Mansfield (J. Phys. C. 10, L55 (1977)). The second is a multi-spin-echo approach in which one plane of k-space is collected during each spin echo. In b...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910320518
更新日期:1994-11-01 00:00:00
abstract:PURPOSE:To accelerate the acquisition of J-resolved proton magnetic resonance spectroscopic imaging (1 H-MRSI) data for high-resolution mapping of brain metabolites and neurotransmitters. METHODS:The proposed method used a subspace model to represent multidimensional spatiospectral functions, which significantly reduc...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.28413
更新日期:2021-01-01 00:00:00
abstract::The relative distributions of N-acetylaspartate (NAA) + N-acetylaspartylglutamate (NAAG), creatine + phosphocreatine (Cr/PCr), and choline (Cho) in the gray and white matter of human brain were determined by utilizing proton magnetic resonance spectroscopic imaging (SI). The SI data was processed using an automated sp...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910330603
更新日期:1995-06-01 00:00:00
abstract::Applications of fMRI in functional brain imaging are mainly confined to single subject designs, prohibiting the assessment of subject or group by condition interactions (i.e., differential activations) or areas of conjoint activation. In this paper a framework for fMRI group designs, using statistical parametric mappi...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910380502
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:To develop a new parallel transmit (pTx) pulse design for simultaneous multiband (MB) excitation in order to tackle simultaneously the problems of transmit B1 (B1+) inhomogeneity and total radiofrequency (RF) power, so as to allow for optimal RF excitation when using MB pulses for slice acceleration for high an...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24828
更新日期:2013-09-01 00:00:00
abstract::Variations in T(1ρ) with locking-field strength (T(1ρ) dispersion) may be used to estimate proton exchange rates. We developed a novel approach utilizing the second derivative of the dispersion curve to measure exchange in a model system of cross-linked polyacrylamide gels. These gels were varied in relative compositi...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22947
更新日期:2011-12-01 00:00:00
abstract::Accelerated magnetic resonance imaging techniques reduce signal acquisition time by undersampling k-space. A fundamental problem in accelerated magnetic resonance imaging is the recovery of quality images from undersampled k-space data. Current state-of-the-art recovery algorithms exploit the spatial and temporal stru...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24524
更新日期:2013-09-01 00:00:00
abstract::Gadobutrol (Gd-Bt; Gadovist(R), Schering AG) is a 1-M Gadolinium (Gd)-based contrast agent. Its higher Gd concentration allows for reduction of injection volumes in first pass contrast-enhanced MR angiography (CE-MRA) and should increase bolus sharpness and image quality. However, ambivalent results were reported. In ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21345
更新日期:2008-01-01 00:00:00
abstract::Imaging of stable paramagnetic spin probes in phantom objects and in vivo was evaluated using a RF time domain EPR spectrometer/imager operating at 300 MHz. Projections were collected using static magnetic field gradients and images were reconstructed using filtered back-projection techniques. Results from phantom obj...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/(sici)1522-2594(200003)43:3<375::aid-mrm9>
更新日期:2000-03-01 00:00:00
abstract::It is shown that in vivo measurement of bile water apparent diffusion coefficient (ADC) by diffusion-weighted echo-planar imaging (EPI) in hamster gallbladder is possible providing motion artifact-free ADC values. These ADC values are used to estimate bile viscosity variation induced by normal diets, cholesterol galls...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/1522-2594(200006)43:6<854::aid-mrm11>3.0.c
更新日期:2000-06-01 00:00:00
abstract::The study of brain function using MRI relies on acquisition techniques that are sensitive to different aspects of the hemodynamic response contiguous to areas of neuronal activity. For this purpose different contrasts such as arterial spin labeling (ASL) and blood oxygenation level dependent (BOLD) functional MRI tech...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21039
更新日期:2006-10-01 00:00:00
abstract::The orientation dependence of the free induction decay (FID) of 1H NMR water signal in ex vivo bovine digital flexor tendon at the native level of hydration is reported. Residual dipolar coupling due to the overall tissue anisotropy produces a 6:1 change in the signal intensity as an angle between the long axis of a s...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.20540
更新日期:2005-08-01 00:00:00
abstract::Subject motion during brain magnetic resonance spectroscopy acquisitions generally reduces the magnetic field (B₀) homogeneity across the volume of interest or voxel. This is the case even if prospective motion correction ensures that the voxel follows the head. We introduce a novel method for rapidly mapping linear v...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24129
更新日期:2012-11-01 00:00:00
abstract:PURPOSE:Diffusion-weighted imaging (DWI) and 18 F-fluorodeoxyglucose-positron emission tomography (18 F-FDG-PET) independently correlate with malignancy in breast cancer, but the relationship between their structural and metabolic metrics is not completely understood. This study spatially correlates diffusion, perfusio...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.26505
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:A multi-turn transmit surface coil design was presented to improve B1 efficiency when used with current source amplification. METHODS:Three different coil designs driven by an on-coil current-mode class-D amplifier with current envelope feedback were tested on the benchtop and through imaging in a 1.5 T scanne...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24612
更新日期:2013-04-01 00:00:00
abstract::We constructed a device to compress small samples of articular cartilage while the samples were imaged in a 1.5 T imager. With the use of a piezoelectric piston, the device compressed 1-cm-diameter cylindrical samples of articular cartilage (200 microm) at a rate of 2 Hz. Simultaneously, we imaged the samples with a d...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.20439
更新日期:2005-05-01 00:00:00
abstract::Pattern recognition techniques (factor analysis and neural networks) were used to investigate and classify human brain tumors based on the 1H NMR spectra of chemically extracted biopsies (n = 118). After removing information from lactate (because of variable ischemia times), unsupervised learning suggested that the sp...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910390604
更新日期:1998-06-01 00:00:00
abstract::Signal-to-noise ratio (SNR) is a major challenge to sodium magnetic resonance imaging. Phased array coils have been shown significantly improving SNR in proton imaging over volume coils. This study investigates SNR advantage of a 15-channel array head coil (birdcage volume coil for transmit/receive and 15-channel arra...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24192
更新日期:2012-12-01 00:00:00
abstract:PURPOSE:Because of short signal lifetimes and respiratory motion, 3D lung MRI is still challenging today. Zero-TE (ZTE) pulse sequences offer promising solutions as they overcome the issue of short T 2 ∗ ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.28483
更新日期:2021-02-01 00:00:00
abstract::Atherosclerosis initially develops predominantly at the aortic root and carotid origin, where effective visualization in mice requires efficient cardiac and respiratory gating. The present study sought to first compare the high-resolution MRI gating performance of two digital gating strategies using: 1) separate cardi...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21308
更新日期:2007-12-01 00:00:00
abstract:PURPOSE:Sickle cell anemia is a blood disorder that alters the morphology and the oxygen affinity of the red blood cells. Cerebral oxygen extraction fraction measurements using quantitative BOLD contrast have been used for assessing inadequate oxygen delivery and the subsequent risk of ischemic stroke in sickle cell an...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.28668
更新日期:2021-01-21 00:00:00
abstract::Skeletal muscle triglycerides are markers for insulin resistance in type 2 diabetes. Recently, MR spectroscopy was adapted for in vivo measurement of triglycerides in animal models and for the characterization of new therapeutic approaches. Because of small MR spectroscopy voxel sizes used in skeletal muscles, surface...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22149
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:We aim to leverage the power of deep-learning with high-fidelity training data to improve the reliability and processing speed of hemodynamic mapping with MR fingerprinting (MRF) arterial spin labeling (ASL). METHODS:A total of 15 healthy subjects were studied on a 3T MRI. Each subject underwent 10 runs of a m...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.28560
更新日期:2021-04-01 00:00:00
abstract::Myocardial tagging is a new noninvasive MRI method that allows the study of myocardial motion with high accuracy. However, with conventional tagging techniques tagging contrast is impaired at later heart phases due to longitudinal relaxation. An improved method, called Complementary SPAtial Modulation of Magnetization...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910300207
更新日期:1993-08-01 00:00:00
abstract::In vivo or ex vivo electron paramagnetic resonance imaging (EPRI) is a powerful technique for determining the spatial distribution of free radicals and other paramagnetic species in living organs and tissues. However, applications of EPRI have been limited by long projection acquisition times and the consequent fact t...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24250
更新日期:2013-02-01 00:00:00
abstract::The investigation of flow behavior in coronary arteries is of great importance for an understanding of heart failure and heart regulation mechanisms. The purpose of the present study was to demonstrate that flow velocity can be quantified in the coronary arteries of the isolated rat heart with high-resolution phase co...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.10563
更新日期:2003-09-01 00:00:00