Abstract:
:A basic requirement for the prediction of the potential use of lactic acid bacteria (LAB) in the dairy industry is the identification of specific genes involved in flavour-forming pathways. The probiotic Lactobacillus delbrueckii UFV H2b20 was submitted to a genetic characterisation and phylogenetic analysis of genes involved in protein catabolism. Eight genes belonging to this system were identified, which possess a closely phylogenetic relationship to NCFM strains representative, as it was demonstrated for oppC and oppBII, encoding oligopeptide transport system components. PepC, PepN, and PepX might be essential for growth of LAB, probiotic or not, since the correspondent genes are always present, including in L. delbrueckii UFV H2b20 genome. For pepX gene, a probable link between carbohydrate catabolism and PepX expression may exists, where it is regulated by PepR1/CcpA-like, a common feature between Lactobacillus strains and also in L. delbrueckii UFV H2b20. The well conserved evolutionary history of the ilvE gene is evidence that the pathways leading to branched-chain amino acid degradation, such as isoleucine and valine, are similar among L. delbrueckii subsp. bulgaricus strains and L. delbrueckii UFV H2b20. Thus, the involvement of succinate in flavour formation can be attributed to IlvE activity. The presence of aminopeptidase G in L. delbrueckii UFV H2b20 genome, which is absent in several strains, might improve the proteolytic activity and effectiveness. The nucleotide sequence encoding PepG revealed that it is a cysteine endopeptidase, belonging to Peptidase C1 superfamily; sequence analysis showed 99% identity with L. delbrueckii subsp. bulgaricus ATCC 11842 pepG, whereas protein sequence analysis revealed 100% similarity with PepG from the same organism. The present study proposes a schematic model to explain how the proteolytic system of the probiotic L. delbrueckii UFV H2b20 works, based on the components identified so far.
journal_name
Benef Microbesjournal_title
Beneficial microbesauthors
Do Carmo AP,da Silva DF,De Oliveira MN,Borges AC,De Carvalho AF,De Moraes CAdoi
10.3920/BM2011.0025subject
Has Abstractpub_date
2011-09-01 00:00:00pages
209-20issue
3eissn
1876-2883issn
1876-2891pii
93M81K4W0658Q505journal_volume
2pub_type
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 临床试验,杂志文章,随机对照试验
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