Abstract:
:Thermotolerant Campylobacter spp., specifically Campylobacter jejuni and Campylobacter coli, are the most common bacterial causes of human gastroenteritis in developed countries. Consumption of improperly prepared poultry products and cross contamination are among the main causes of human campylobacteriosis. The aim of this study was to identify lactic acid bacterial (LAB) strains capable of inhibiting C. jejuni growth in initial in vitro trials ('spot-on-lawn' method), as well as in batch fermentation studies mimicking the broiler caecal environment. These experiments served as an indication for using these strains to decrease the capability of Campylobacter to colonise and grow in the chicken caeca during primary production, with the aim of reducing the number of human campylobacteriosis cases. A total of 1,150 LAB strains were screened for anti-Campylobacter activity. Six strains were selected: members of the species Lactobacillus reuteri, Lactobacillus agilis, Lactobacillus helveticus, Lactobacillus salivarius, Enterococcus faecalis and Enterococcus faecium. After treatment with catalase, proteinase K and a-chymotrypsin, anti-Campylobacter activity of cell-free culture supernatant fluid (CSF) for all six strains was retained, which indicated that activity was probably not exerted by bacteriocin production. Based on the activity found in CSF, the compounds produced by the selected strains are secreted and do not require presence of live bacterial producer cells for activity. During initial in vitro fermentation experiments, the E. faecalis strain exhibited the highest inhibitory activity for C. jejuni and was selected for further fermentation experiments. In these experiments we tested for therapeutic or protective effects of the E. faecalis strain against C. jejuni MB 4185 infection under simulated broiler caecal growth conditions. The best inhibition results were obtained when E. faecalis was inoculated before the C. jejuni strain, lowering C. jejuni counts at least one log compared to a positive control. This effect was already observed 6 h after C. jejuni inoculation.
journal_name
Benef Microbesjournal_title
Beneficial microbesauthors
Robyn J,Rasschaert G,Messens W,Pasmans F,Heyndrickx Mdoi
10.3920/BM2012.0021subject
Has Abstractpub_date
2012-12-01 00:00:00pages
299-308issue
4eissn
1876-2883issn
1876-2891pii
C1011133V8858RX1journal_volume
3pub_type
杂志文章abstract::Clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. Our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral Candida species. Sugar utilisation by both lactobacil...
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2018.0080
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abstract::The gut intestinal tract harbours a complex microbiota. Disturbances in the microbiota composition have been associated with several immune dysfunctions such as inflammatory diseases. Specific strains of probiotics have shown to beneficially influence the composition and/or metabolic activity of the endogenous microbi...
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pub_type: 杂志文章,评审
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journal_title:Beneficial microbes
pub_type: 杂志文章,评审
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
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doi:10.3920/BM2013.0016
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journal_title:Beneficial microbes
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2017.0131
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abstract::There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growt...
journal_title:Beneficial microbes
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pub_type: 杂志文章,评审
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journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2010.0031
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 杂志文章
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