Abstract:
:The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.
journal_name
Benef Microbesjournal_title
Beneficial microbesauthors
Fernández S,Fraga M,Silveyra E,Trombert AN,Rabaza A,Pla M,Zunino Pdoi
10.3920/BM2017.0131subject
Has Abstractpub_date
2018-06-15 00:00:00pages
613-624issue
4eissn
1876-2883issn
1876-2891journal_volume
9pub_type
杂志文章abstract::Vaccination is one of the most important prevention tools providing protection against infectious diseases especially in children below the age of five. According to estimates, more than 5 million lives are saved annually by the implementation of six standard vaccines, including diphtheria, hepatitis B, Haemophilus in...
journal_title:Beneficial microbes
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
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abstract::The gut is hypothesised to play an important role in the development and progression of sepsis. It is however unknown whether the gut microbiome and the gut barrier function is already altered early in sepsis development and whether it is possible to modulate the microbiome in early sepsis. Therefore, a randomised, do...
journal_title:Beneficial microbes
pub_type: 杂志文章,随机对照试验
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更新日期:2019-04-19 00:00:00
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journal_title:Beneficial microbes
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Beneficial microbes
pub_type: 杂志文章,评审
doi:10.3920/BM2018.0126
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2017.0035
更新日期:2018-09-18 00:00:00
abstract::The probiotic strain Bifidobacterium bifidum YIT 10347 has been demonstrated to inhibit Helicobacter pylori activity, prevent injury to the gastric mucosa, and improve general gastric malaise symptoms in H. pylori positive patients. This study aimed to investigate the adhering activity and localisation of B. bifidum Y...
journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2015.0138
更新日期:2016-06-01 00:00:00
abstract::The present work looks at the role of phytate-degrading Pediococcus pentosaceus CFR R123 application in functional foods to evaluate the fate of phytate and calcium solubility during fermentation. Under standard conditions, CFR R123 grown in modified MRS containing sodium phytate CFR R123 showed 43% degradation of sod...
journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2010.0031
更新日期:2011-03-01 00:00:00
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 杂志文章,评审
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
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journal_title:Beneficial microbes
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:Beneficial microbes
pub_type: 杂志文章
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更新日期:2017-08-24 00:00:00
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journal_title:Beneficial microbes
pub_type: 杂志文章,随机对照试验
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更新日期:2018-06-15 00:00:00
abstract::Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel f...
journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2017.0183
更新日期:2018-12-07 00:00:00
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journal_title:Beneficial microbes
pub_type: 杂志文章
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journal_title:Beneficial microbes
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2018-01-29 00:00:00
abstract::Hypercholesterolemia is a main risk factor of cardiovascular disease. Probiotics are a safe approach to reduce elevated cholesterol without any deleterious effect to human health. Saccharomyces boulardii CNCM I-745 probiotic properties are well documented in a context of intestinal dysbiosis. Recent in vitro and precl...
journal_title:Beneficial microbes
pub_type: 杂志文章
doi:10.3920/BM2018.0134
更新日期:2019-05-28 00:00:00