Strategies for the evaluation and selection of potential vaginal probiotics from human sources: an exemplary study.

Abstract:

:During the last years, the application of probiotics in gynaecological clinical practice has gained increasing relevance regarding therapy and prevention. This trend has also provoked the need for having tailored pharmaceutical preparations containing powerful microbial strains with defined properties. For the development of such preparations, several factors and criteria have to be considered, thereby not only focusing on identity and safety aspects as well as individual properties of the bacterial strains, but also on technological issues, such as stability and targeted release from the preparation. Against this background, this report exemplarily addresses the development procedure of a probiotic bacterial formulation for gynaecological application, covering the search for suitable strains, assessing their microbiological, molecular biological and physiological characterisation, and the selection for their use in clinical trials. In detail, starting with 127 presumptive lactobacilli isolates of vaginal origin, a step-by-step selection of candidate strains meeting special criteria was thoroughly examined, finally leading to a preparation consisting of four individual Lactobacillus strains that possess particular significance in women's urogenital health. Relevant issues and quality criteria of probiotic preparations used in gynecology are addressed and exemplarily introduced.

journal_name

Benef Microbes

journal_title

Beneficial microbes

authors

Domig KJ,Kiss H,Petricevic L,Viernstein H,Unger F,Kneifel W

doi

10.3920/BM2013.0069

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

263-72

issue

3

eissn

1876-2883

issn

1876-2891

pii

J637654542315368

journal_volume

5

pub_type

杂志文章
  • Effect of a yoghurt drink containing Lactobacillus strains on bacterial vaginosis in women - a double-blind, randomised, controlled clinical pilot trial.

    abstract::Bacterial vaginosis (BV) is characterised by a depletion of lactobacilli in favour of an overgrowth of anaerobic bacteria. It is associated with increased risk for urogenital infections and abortion. In this study we assessed the effect of a yoghurt drink containing Lactobacillus strains on BV. The strains had been is...

    journal_title:Beneficial microbes

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3920/BM2017.0018

    authors: Laue C,Papazova E,Liesegang A,Pannenbeckers A,Arendarski P,Linnerth B,Domig KJ,Kneifel W,Petricevic L,Schrezenmeir J

    更新日期:2018-01-29 00:00:00

  • Prebiotics to manage the microbial control of energy homeostasis.

    abstract::The prevalence of obesity is continuously growing and has reached epidemic proportions. It is clear that current methods to combat obesity are not effective enough to reduce the problem. Therefore, further investigation is needed to develop new strategies. Recent research pointed out a potential role of the microbial ...

    journal_title:Beneficial microbes

    pub_type: 杂志文章,评审

    doi:10.3920/BM2011.0020

    authors: Grootaert C,Marzorati M,Van den Abbeele P,Van de Wiele T,Possemiers S

    更新日期:2011-12-01 00:00:00

  • Genes involved in protein metabolism of the probiotic lactic acid bacterium Lactobacillus delbrueckii UFV H2b20.

    abstract::A basic requirement for the prediction of the potential use of lactic acid bacteria (LAB) in the dairy industry is the identification of specific genes involved in flavour-forming pathways. The probiotic Lactobacillus delbrueckii UFV H2b20 was submitted to a genetic characterisation and phylogenetic analysis of genes ...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2011.0025

    authors: Do Carmo AP,da Silva DF,De Oliveira MN,Borges AC,De Carvalho AF,De Moraes CA

    更新日期:2011-09-01 00:00:00

  • Randomised double blind placebo controlled trial on Lactobacillus reuteri DSM 17938: improvement in symptoms and bowel habit in functional constipation.

    abstract::Dysbiosis may contribute to constipation and its symptoms, therefore probiotic administration could improve significantly gut health and functions. The aim of the study was to investigate the effects of a long-lasting administration of Lactobacillus reuteri DSM 17938 (LR DSM 17938) on symptoms and quality of life (QoL...

    journal_title:Beneficial microbes

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3920/BM2017.0049

    authors: Riezzo G,Orlando A,D'Attoma B,Linsalata M,Martulli M,Russo F

    更新日期:2018-01-29 00:00:00

  • Probiotic modulation of dendritic cells and T cell responses in the intestine.

    abstract::Over the past decade it has become clear that probiotic and commensal interactions with mucosal dendritic cells in the lamina propria or epithelial cells lining the mucosa can modulate specific functions of the mucosal immune system. Innate pattern-recognition receptors such as TLRs, NLRs and CLRs play a crucial role ...

    journal_title:Beneficial microbes

    pub_type: 杂志文章,评审

    doi:10.3920/BM2010.0029

    authors: Meijerink M,Wells JM

    更新日期:2010-11-01 00:00:00

  • The administration of probiotics and synbiotics in immune compromised adults: is it safe?

    abstract::This study aimed to systematically evaluate safety of probiotics and synbiotics in immune compromised adults (≥18 years). Safety was analysed using the Common Terminology Clinical Adverse Events (CTCAE version 4.0) classification, thereby providing an update on previous reports using the most recent available clinical...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2014.0079

    authors: Van den Nieuwboer M,Brummer RJ,Guarner F,Morelli L,Cabana M,Claasen E

    更新日期:2015-03-01 00:00:00

  • Anti-inflammatory properties of probiotic bacteria on Salmonella-induced IL-8 synthesis in enterocyte-like Caco-2 cells.

    abstract::Invasion of the gut by pathogenic Salmonella leads to production of IL-8 that initiates inflammatory reactions to combat the bacterium. However, its persistent production causes tissue damage and interventions that suppress IL-8 production prevent tissue damage. We hypothesised that probiotics could mediate their bene...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2009.0021

    authors: Malago JJ,Tooten PC,Koninkx JF

    更新日期:2010-06-01 00:00:00

  • The serotonin receptor mediates changes in autonomic neurotransmission and gastrointestinal transit induced by heat-killed Lactobacillus brevis SBC8803.

    abstract::Lactobacilli exhibit several health benefits in mammals, including humans. Our previous reports established that heat-killed Lactobacillus brevis SBC8803 (SBC8803) increased both efferent gastric vagal nerve activity and afferent intestinal vagal nerve activity in rats. We speculated that this strain could be useful f...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2015.0031

    authors: Horii Y,Nakakita Y,Misonou Y,Nakamura T,Nagai K

    更新日期:2015-01-01 00:00:00

  • Milk fermented by Lactobacillus species from Brazilian artisanal cheese protect germ-free-mice against Salmonella Typhimurium infection.

    abstract::Ingestion of milks fermented by Lactobacillus strains showing probiotic properties is an important tool to maintain gastrointestinal health. In this study, Lactobacillus rhamnosus D1 and Lactobacillus plantarum B7, isolated from Brazilian artisanal cheese, were used as starters for the functional fermented milks to as...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2016.0163

    authors: Acurcio LB,Sandes SHC,Bastos RW,Sant'anna FM,Pedroso SHSP,Reis DC,Nunes ÁC,Cassali GD,Souza MR,Nicoli JR

    更新日期:2017-08-24 00:00:00

  • Lactobacillus paracasei K5 displays adhesion, anti-proliferative activity and apoptotic effects in human colon cancer cells.

    abstract::Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel f...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2017.0183

    authors: Chondrou P,Karapetsas A,Kiousi DE,Tsela D,Tiptiri-Kourpeti A,Anestopoulos I,Kotsianidis I,Bezirtzoglou E,Pappa A,Galanis A

    更新日期:2018-12-07 00:00:00

  • Propionic acid and its esterified derivative suppress the growth of methicillin-resistant Staphylococcus aureus USA300.

    abstract::Previously, we demonstrated that Propionibacterium acnes, a human skin commensal bacterium, ferments glycerol into short-chain fatty acids, including propionic acid. Propionic acid suppressed the growth of Staphylococcus aureus USA300, a community-acquired methicillin-resistant bacterium, in vitro and in vivo. In this...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2013.0031

    authors: Wang Y,Dai A,Huang S,Kuo S,Shu M,Tapia CP,Yu J,Two A,Zhang H,Gallo RL,Huang CM

    更新日期:2014-06-01 00:00:00

  • Saccharomyces boulardii CNCM I-745 changes lipidemic profile and gut microbiota in a hamster hypercholesterolemic model.

    abstract::Hypercholesterolemia is a main risk factor of cardiovascular disease. Probiotics are a safe approach to reduce elevated cholesterol without any deleterious effect to human health. Saccharomyces boulardii CNCM I-745 probiotic properties are well documented in a context of intestinal dysbiosis. Recent in vitro and precl...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2018.0134

    authors: Briand F,Sulpice T,Giammarinaro P,Roux X

    更新日期:2019-05-28 00:00:00

  • Resistant starch, large bowel fermentation and a broader perspective of prebiotics and probiotics.

    abstract::The metabolic end products of the large bowel microbiota contribute significantly to human health. After weaning to solid foods, some of the most important of these are the short chain fatty acids (SCFA) produced by the fermentation of undigested dietary components and endogenous secretions. The main SCFA are acetate,...

    journal_title:Beneficial microbes

    pub_type: 杂志文章,评审

    doi:10.3920/BM2010.0041

    authors: Bird AR,Conlon MA,Christophersen CT,Topping DL

    更新日期:2010-11-01 00:00:00

  • Immune modulation property of Lactobacillus paracasei NCC2461 (ST11) strain and impact on skin defences.

    abstract::The gut intestinal tract harbours a complex microbiota. Disturbances in the microbiota composition have been associated with several immune dysfunctions such as inflammatory diseases. Specific strains of probiotics have shown to beneficially influence the composition and/or metabolic activity of the endogenous microbi...

    journal_title:Beneficial microbes

    pub_type: 杂志文章,评审

    doi:10.3920/BM2013.0014

    authors: Benyacoub J,Bosco N,Blanchard C,Demont A,Philippe D,Castiel-Higounenc I,Guéniche A

    更新日期:2014-06-01 00:00:00

  • Probiotic properties of native Lactobacillus spp. strains for dairy calves.

    abstract::The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potent...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2017.0131

    authors: Fernández S,Fraga M,Silveyra E,Trombert AN,Rabaza A,Pla M,Zunino P

    更新日期:2018-06-15 00:00:00

  • Consumer perception of beneficial effects of probiotics for human health.

    abstract::The purpose of this study was to evaluate the knowledge, perception and buying behaviour of probiotics. 72 participants in Middelburg, the Netherlands, filled out a detailed questionnaire regarding probiotics and their customer and consumer behaviour. It can be concluded from this study that the concept of probiotics ...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2012.0050

    authors: Rijkers GT,Bimmel D,Grevers D,den Haan N,Hristova Y

    更新日期:2013-03-01 00:00:00

  • Bifidobacterium breve prevents necrotising enterocolitis by suppressing inflammatory responses in a preterm rat model.

    abstract::Necrotising enterocolitis (NEC) is associated with inflammatory responses and barrier dysfunction in the gut. In this study, we investigated the effect of Bifidobacterium breve M-16V on factors related to NEC development using an experimental rat model. Caesarean-sectioned rats were given formula milk with or without ...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2015.0035

    authors: Satoh T,Izumi H,Iwabuchi N,Odamaki T,Namba K,Abe F,Xiao JZ

    更新日期:2016-02-01 00:00:00

  • Galacto-oligosaccharides ameliorate dysbiotic Bifidobacteriaceae decline in Japanese patients with type 2 diabetes.

    abstract::Gut microbiota affects the host's metabolism, and it is suggested that there are differences in gut microbiota composition between patients with type 2 diabetes and healthy individuals. Additionally, dysbiosis may increase the concentration of lipopolysaccharides (LPS), causing metabolic endotoxemia, which induces imp...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2016.0230

    authors: Gonai M,Shigehisa A,Kigawa I,Kurasaki K,Chonan O,Matsuki T,Yoshida Y,Aida M,Hamano K,Terauchi Y

    更新日期:2017-10-13 00:00:00

  • Human milk mycobiota composition: relationship with gestational age, delivery mode, and birth weight.

    abstract::Intestinal and human milk microbiota studies during infancy have shown variations according to geographical location, delivery mode, gestational age, and mother-related factors during pregnancy. In this study, we performed metagenomic mycobiota analyses of 44 transient and mature human milk among five different groups...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2019.0158

    authors: Dinleyici M,Pérez-Brocal V,Arslanoglu S,Aydemir O,Ozumut SS,Tekin N,Vandenplas Y,Moya A,Dinleyici EC

    更新日期:2020-03-27 00:00:00

  • On the importance of intraindividual variation in nutritional research.

    abstract::Nutritional intervention studies, like those with pre- and probiotics, are often hampered by low effect sizes, reducing the power to demonstrate potential efficacy. Here, we perform computer simulations of a hypothetical clinical trial using such an intervention in order to elucidate determining factors that can be in...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2020.0044

    authors: Larsen OFA,Claassen E,Brummer RJ

    更新日期:2020-10-12 00:00:00

  • Gut microbiota in health and disease: an overview focused on metabolic inflammation.

    abstract::In concern to the continuously rising global prevalence of obesity, diabetes and associated diseases, novel preventive and therapeutic approaches are urgently required. However, to explore and develop such innovative strategies, a meticulous comprehension of the biological basis of these diseases is extremely importan...

    journal_title:Beneficial microbes

    pub_type: 杂志文章,评审

    doi:10.3920/bm2015.0062

    authors: Nagpal R,Kumar M,Yadav AK,Hemalatha R,Yadav H,Marotta F,Yamashiro Y

    更新日期:2016-01-01 00:00:00

  • Phytate-degrading Pediococcus pentosaceus CFR R123 for application in functional foods.

    abstract::The present work looks at the role of phytate-degrading Pediococcus pentosaceus CFR R123 application in functional foods to evaluate the fate of phytate and calcium solubility during fermentation. Under standard conditions, CFR R123 grown in modified MRS containing sodium phytate CFR R123 showed 43% degradation of sod...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2010.0031

    authors: Raghavendra P,Ushakumari SR,Halami PM

    更新日期:2011-03-01 00:00:00

  • Cross-feeding between Bifidobacterium infantis and Anaerostipes caccae on lactose and human milk oligosaccharides.

    abstract::The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health. At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling. The well-orchestrated transition is imp...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2020.0005

    authors: Chia LW,Mank M,Blijenberg B,Bongers RS,van Limpt K,Wopereis H,Tims S,Stahl B,Belzer C,Knol J

    更新日期:2020-11-16 00:00:00

  • Bifidobacteria and its rice fermented products on diet induced obese mice: analysis of physical status, serum profile and gene expressions.

    abstract::Obesity is highly correlated with the dysbiosis of intestinal microbiota, and bifidobacteria are one of the soft targets of this metabolic syndrome. The aim of this study is to evaluate the efficacy of Bifidobacterium sp. MKK4 and rice-based fermented foods on physical, haematological, gut microbiota and lypogenic-lyp...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2017.0056

    authors: Ray M,Hor PK,Ojha D,Soren JP,Singh SN,Mondal KC

    更新日期:2018-04-25 00:00:00

  • Comparison of detection methods for vaginal lactobacilli.

    abstract::Vaginal lactobacilli offer protection against microbiota imbalance and genitourinary tract infections. We compared vaginal lactobacilli in 50 Estonian women of child-bearing age applying culture-based methods, quantitative PCR and next-generation sequencing (NGS). The culture-based methods found three different lactob...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2014.0154

    authors: Smidt I,Kiiker R,Oopkaup H,Lapp E,Rööp T,Truusalu K,Štšepetova J,Truu J,Mändar R

    更新日期:2015-01-01 00:00:00

  • The effects of the Lactobacillus casei strain on obesity in children: a pilot study.

    abstract::There are few data regarding the role of probiotics as a dietary intervention in the management of obesity in children. An open prospective examination was conducted to clarify the effects of Lactobacillus casei strain Shirota (LcS)-containing beverages in obese children. We compared the intestinal microbiota and orga...

    journal_title:Beneficial microbes

    pub_type: 临床试验,杂志文章

    doi:10.3920/BM2016.0170

    authors: Nagata S,Chiba Y,Wang C,Yamashiro Y

    更新日期:2017-08-24 00:00:00

  • Effect of Saccharomyces cerevisiae strain UFMG A-905 in experimental model of inflammatory bowel disease.

    abstract::In the present study, the protective potential of Saccharomyces cerevisiae strain UFMG A-905 was evaluated in a murine model of acute ulcerative colitis (UC). Six groups of Balb/c mice were used: not treated with yeast and not challenged with dextran sulphate sodium (DSS) (control); treated with S. cerevisiae UFMG A-9...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2015.0018

    authors: Tiago FC,Porto BA,Ribeiro NS,Moreira LM,Arantes RM,Vieira AT,Teixeira MM,Generoso SV,Nascimento VN,Martins FS,Nicoli JR

    更新日期:2015-01-01 00:00:00

  • Influence of Lactobacillus brevis 15 and Lactobacillus plantarum 13 on blood glucose and body weight in rats after high-fructose diet.

    abstract::In recent years, many authors have investigated the possible antidiabetic effect of lactic acid bacteria. Lactobacillus species constitute a major part of the lactic acid bacteria group and have been found to exhibit beneficial effects on the development of diabetes and its complications. In the current study, we inve...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2014.0012

    authors: Yakovlieva M,Tacheva T,Mihaylova S,Tropcheva R,Trifonova K,Toleкova A,Danova S,Vlaykova T

    更新日期:2015-01-01 00:00:00

  • Modulatory effect of three probiotic strains on infants' gut microbial composition and immunological parameters on a placebo-controlled, double-blind, randomised study.

    abstract::The gut microbiota plays a crucial role in gastrointestinal health. Current use of probiotics is aimed at modulating the bacterial gut composition to alleviate specific diseases. The safety and tolerance of three probiotic strains (Bifidobacterium longum subsp. infantis R0033, Lactobacillus helveticus R0052 and Bifido...

    journal_title:Beneficial microbes

    pub_type: 杂志文章,随机对照试验

    doi:10.3920/BM2017.0132

    authors: De Andrés J,Manzano S,García C,Rodríguez JM,Espinosa-Martos I,Jiménez E

    更新日期:2018-06-15 00:00:00

  • Gut bacterial composition in a mouse model of Parkinson's disease.

    abstract::The mechanism of neurodegeneration in Parkinson's disease (PD) remains unknown but it has been hypothesised that the intestinal tract could be an initiating and contributing factor to the neurodegenerative processes. In PD patients as well as in animal models for PD, alpha-synuclein-positive enteric neurons in the col...

    journal_title:Beneficial microbes

    pub_type: 杂志文章

    doi:10.3920/BM2017.0202

    authors: Perez-Pardo P,Dodiya HB,Engen PA,Naqib A,Forsyth CB,Green SJ,Garssen J,Keshavarzian A,Kraneveld AD

    更新日期:2018-09-18 00:00:00