Abstract:
:We studied penicillin-binding protein (PBP) profiles of two Pseudomonas aeruginosa strains stably derepressed for constitutive, type Id beta-lactamase overproduction. Substantial levels of beta-lactamase were found to be strongly associated with isolated inner membranes of these two strains, as well as from a plasmid-encoded, TEM-2 beta-lactamase-producing control strain of P. aeruginosa. The inner membrane-associated beta-lactamase resulted in significant decreases in the intensity on autoradiographs of PBPs labelled with 35S-penicillin, yielding spurious PBP profiles for these strains. Inner membrane beta-lactamases could be substantially removed by a sonication-ultracentrifugation step, producing the bona fide PBP profiles.
journal_name
Chemotherapyjournal_title
Chemotherapyauthors
Parr TR Jr,Chan L,Bayer ASdoi
10.1159/000238612subject
Has Abstractpub_date
1988-01-01 00:00:00pages
478-83issue
6eissn
0009-3157issn
1421-9794journal_volume
34pub_type
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