Isolation of viable Toxoplasma gondii from organs and Brazilian commercial meat cuts of experimentally infected pigs.

Abstract:

:The present study evaluated the distribution and viability of Toxoplasma gondii tissue cysts in the organs and Brazilian commercial cuts of experimentally infected pigs. The pigs were infected with 3 × 103 oocysts of the T. gondii isolate TgCkBr57 (Type BrII). Mouse bioassays were performed on the brain, retina, tongue, diaphragm, and heart as well as the following muscle cuts: loin (longissimus), coppa (longissimus, spinalis dorsi, rhomboideus), tenderloin (psoas major), outside flat (biceps femoris), topside (semimembranosus), and top sirloin (gluteus medius). Toxoplasma gondii was isolated from the coppa, heart, diaphragm, and tongue of three pigs; from the tenderloin, outside flat, and brain of two pigs; and from the top sirloin and loin of one pig. Thus, the viability of T. gondii cysts was observed in all of the organs and cuts evaluated (except for the topside and retina), demonstrating the broad distribution of this parasite in pig organs and commercial meat cuts, and the importance of this species as a source of human infection.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Alves BF,Oliveira S,Soares HS,Pena HFJ,Conte-Junior CA,Gennari SM

doi

10.1007/s00436-019-06229-6

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

1331-1335

issue

4

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-019-06229-6

journal_volume

118

pub_type

杂志文章
  • Immunomodulation of liver injury by Ascaris suum extract in an experimental model of autoimmune hepatitis.

    abstract::Adult worm extract from Ascaris suum (Asc) has immunosuppressive activity and elicits Th2/IL-4/IL-10 response. This study evaluated the prophylactic and therapeutic effect of Asc in a murine model of concanavalin A (ConA)-induced autoimmune hepatitis (AIH). BALB/c mice received ConA, iv, (20 mg/kg), and three groups o...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-3994-6

    authors: Nascimento WC,Silva RP,Fernandes ES,Silva MC,Holanda GC,Santos PA,Albuquerque MP,Costa VA,Pontes-Filho NT,Souza VO

    更新日期:2014-09-01 00:00:00

  • Seasonal occurrence and metrical variability of Gyrodactylus rhodei Zitnan 1964 (Monogenea, Gyrodactylidae).

    abstract::The seasonal dynamics of Gyrodactylus rhodei, a monogenean ectoparasite of bitterling (Rhodeus sericeus), was studied from June 2000 to May 2001 in the Kyjovka River, Czech Republic. A negative relationship between prevalence and intensity of infection of G. rhodei and water temperature was found. Metrical variability...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-1311-0

    authors: Dávidová M,Jarkovský J,Matejusová I,Gelnar M

    更新日期:2005-04-01 00:00:00

  • The ITS2 ribosomal DNA of Anopheles beklemishevi and further remarks on the phylogenetic relationships within the Anopheles maculipennis group of species (Diptera: Culicidae).

    abstract::Anopheles beklemishevi specimens from Russia were analysed by their ITS2 ribosomal DNA sequence to amend and to specify the phylogenetic tree of the Anopheles maculipennis species complex. Surprisingly, with 638 base pairs, the ITS2 regions of all the 34 An beklemishevi specimens examined were considerably longer than...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-1393-8

    authors: Kampen H

    更新日期:2005-09-01 00:00:00

  • Paramphistomum daubneyi and Fasciola hepatica: the redial burden and cercarial shedding in Lymnaea truncatula subjected to successive unimiracidial cross-exposures.

    abstract::The development of redial burden and cercarial shedding were studied in two groups of Lymnaea truncatula subjected to successive cross-exposures to one miracidium of Paramphistomum daubneyi and one of Fasciola hepatica per snail, or vice versa. The results were compared with those obtained in controls subjected to two...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050175

    authors: Augot D,Abrous M,Rondelaud D,Dreyfuss G

    更新日期:1996-01-01 00:00:00

  • Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga.

    abstract::The effectiveness of a bacteriocin-like substance (BLS) produced by Bacillus amyloliquefaciens was tested against Acanthamoeba polyphaga strains, and its cytotoxic potential on Vero cells was investigated. Amebicidal activity of the purified BLS was tested by plate bioassays with concentrations ranging from 12.5 to 6,...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-2114-5

    authors: Benitez LB,Caumo K,Brandelli A,Rott MB

    更新日期:2011-03-01 00:00:00

  • Toxoplasma gondii nucleus coding apicoplast protein ACP synthesis and trafficking in delayed death.

    abstract::This study aimed to explore Toxoplasma gondii nucleus coding apicoplast protein acyl carrier protein (ACP) synthesis and trafficking in delayed death. The recombinant T. gondii ACP was expressed by prokaryotic expression method, and anti-ACP polyclonal antibody was obtained from rabbit immune. T. gondii "delayed death...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-4281-2

    authors: Wu L,Shen J,Zhou Y,Wang X,Wu L,Jiang X,Chen S

    更新日期:2015-03-01 00:00:00

  • A comparative ultrastructural and histochemical study of the metacercarial cyst walls of four species of Paragonimus (Troglotrematidae: Trematoda).

    abstract::The metacercarial cyst walls of Paragonimus westermani, P. miyazakii, P. ohirai, and P. iloktsuenensis were examined using ultrastructural and histochemical techniques. The cyst walls of P. westermani, P. miyazakii, and P. ohirai were found to have five distinct layers, whereas the wall of P. iloktsuenensis had only t...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00931563

    authors: Lou YS,Fujino T,Morita K,Ishii Y

    更新日期:1992-01-01 00:00:00

  • Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics.

    abstract::The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050519

    authors: Kerboeuf D,Chambrier P,Le Vern Y,Aycardi J

    更新日期:1999-02-01 00:00:00

  • The neuro-muscular system in continuously swimming cercariae from Belarus. I Xiphidiocercariae.

    abstract::The neuromuscular system (NMS) in cercariae of Neoastiotrema trituri, Plagiorchis elegans, Omphalometra flexuosa, Skrjabinoeces similis and Prosthogonimus ovatus was studied with immunocytochemical methods and confocal scanning laser microscopy. The patterns of F-actin in the musculature, 5-HT immunoreactive (IR), FMR...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-012-3044-1

    authors: Tolstenkov OO,Akimova LN,Terenina NB,Gustafsson MK

    更新日期:2012-11-01 00:00:00

  • Lipoptena cervi (Diptera), a potential vector of Megatrypanum trypanosomes of deer (Cervidae).

    abstract::In three different areas in northern Germany, hippoboscids were collected from red deer (Cervus elaphus). Typanosomatids were demonstrated in the midgut and hindgut of 9/37 Lipoptena cervi. The trypanosomatids were morphologically similar to vector forms of the subgenus Megatrypanum that had been found in tabanids at ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00928691

    authors: Böse R,Petersen K

    更新日期:1991-01-01 00:00:00

  • Development of improved methods for delivery of Trichuris muris to the laboratory mouse.

    abstract::Murine immunological responses to experimental infection with Trichuris muris and the effects of the resident microbiota on these responses are of increasing interest. For these studies, accurate dose delivery and improved sterilization of inocula are essential to prevent co-infection with unknown contaminants. We fou...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-1978-8

    authors: Kopper JJ,Mansfield LS

    更新日期:2010-10-01 00:00:00

  • Genetic regulation of arrested development in nematodes: are age-1 and daf-gene orthologs present in Dictyocaulus viviparus?

    abstract::In opposite to the free-living soil nematode Caenorhabditis elegans, the genetic regulation of hypobiosis or inhibited or arrested development in parasitic nematodes is completely unknown. In C. elegans, the daf-genes or the age-1 gene are of major importance in signaling pathways regulating arrested development. To i...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0594-8

    authors: Strube C,von Samson-Himmelstjerna G,Schnieder T

    更新日期:2007-09-01 00:00:00

  • Serological detection of exposure to Cryptosporidium parvum in cattle by ELISA and its evaluation in relation to coprological tests.

    abstract::We evaluated serum examination as an alternative to fecal analysis for the diagnosis of exposure to Cryptosporidium parvum in cattle. The accuracy of the serum ELISA was compared to the combined results of concentration flotation microscopy and fecal enzyme immunoassay. The expected performance of the serum ELISA at d...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-002-0665-9

    authors: Nydam DV,Lindergard G,Guard CL,Schaaf SL,Wade SE,Mohammed HO

    更新日期:2002-09-01 00:00:00

  • Validation of a new immunofluorescence antibody test for the detection of Leishmania infantum infection in cats.

    abstract::The prevalence data of Leishmania infantum infection in cats are characterized by a large variability mainly attributed to the differences in diagnostic techniques. In the absence of consensus about the method of choice for diagnosing feline leishmaniosis, the performance of a new immunofluorescence antibody test (IFA...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-020-06627-1

    authors: Iatta R,Trerotoli P,Lucchese L,Natale A,Buonavoglia C,Nachum-Biala Y,Baneth G,Otranto D

    更新日期:2020-04-01 00:00:00

  • Molecular characterization and expression pattern of an odorant receptor from the myiasis-causing blowfly, Lucilia sericata (Diptera: Calliphoridae).

    abstract::The blowfly Lucilia sericata (Diptera: Calliphoridae) is a facultative ectoparasite that causes myiasis in both man and animals, leading to serious human health problems and economic losses in the livestock industry. Like other insects, olfaction of this species plays an important role in host location and is presumab...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2563-5

    authors: Wang X,Zhong M,Wen J,Cai J,Jiang H,Liu Y,Aly SM,Xiong F

    更新日期:2012-02-01 00:00:00

  • Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis.

    abstract::Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukar...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-1742-0

    authors: Liu MM,Yuan ZG,Peng GH,Zhou DH,He XH,Yan C,Yin CC,He Y,Lin RQ,Song HQ,Zhu XQ

    更新日期:2010-04-01 00:00:00

  • An in vitro model to evaluate the cytokine response in Echinococcus infections.

    abstract::With the aim of proposing an alternative model to animal experimentation, we investigated cytokine production in response to antigens in an in vitro system. This is a co-culture system of healthy human leukocytes and enterocyte-like Caco-2 cells. The antigens tested, EgA31, EgTrp, and FABP1, are candidates for vaccine...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-004-1075-y

    authors: Fraize M,Sarciron ME,Saboulard D,Azzouz S,Debard AL,Bosquet G,Petavy AF

    更新日期:2004-04-01 00:00:00

  • Eosinophil chemotactic chemokine profilings of the brain from permissive and non-permissive hosts infected with Angiostrongylus cantonenis.

    abstract::Angiostrongylus cantonensis invasion primarily cause heavy or negligible eosinophic meningitis and meningoencephalitis in the brain of non-permissive and permissive hosts, respectively. Chemokines are effective leukocyte chemoattractants and may play an essential role in mediating eosinophil recruitment in angiostrong...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3683-x

    authors: Li S,Yang F,Ji P,Zeng X,Wu X,Wei J,Ouyang L,Liang J,Zheng H,Wu Z,Lv Z

    更新日期:2014-02-01 00:00:00

  • Efficacy of emodepside plus praziquantel tablets (Profender tablets for dogs) against mature and immature adult Ancylostoma caninum and Uncinaria stenocephala infections in dogs.

    abstract::This paper reports the efficacy of a novel flavoured tablet formulation of emodepside plus praziquantel (Profender tablets for dogs) against mature and immature adult hookworms (Ancylostoma caninum and Uncinaria stenocephala) in dogs. The tablets were used at the minimum recommended dose of 1 mg emodepside and 5 mg pr...

    journal_title:Parasitology research

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00436-009-1490-1

    authors: Schimmel A,Altreuther G,Schroeder I,Charles S,Cruthers L,Ketzis J,Kok DJ,Kraemer F,McCall JW,Krieger KJ

    更新日期:2009-08-01 00:00:00

  • Seasonal dynamics of Trichodina spp. on whiting (Merlangius merlangus)in relation to organic pollution on the eastern Black Sea coast of Turkey.

    abstract::It was determined that there is a relationship between prevalence and mean densities of Trichodina spp. on Merlangius merlangus and organic pollution, measured as levels of nitrite, nitrate and phosphate in the surrounding environment. Monthly, two left anterior gill arcs of 60 M. merlangus (unless otherwise stated) c...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-1346-2

    authors: Ogut H,Palm HW

    更新日期:2005-06-01 00:00:00

  • Morphological and molecular observations on the status of Crassicauda magna, a parasite of the subcutaneous tissues of the pygmy sperm whale, with a re-evaluation of the systematic relationships of the genus Crassicauda.

    abstract::Members of the genus Crassicauda (Nematoda: Spirurida) are parasites of the body tissues of whales and dolphins. Owing to the large size of worms and difficulties in the recovery of entire nematodes from the tissues of hosts, limited information is available on morphological descriptions of both male and female worms....

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-4245-6

    authors: Jabbar A,Beveridge I,Bryant MS

    更新日期:2015-03-01 00:00:00

  • Ultrastructural observations of the attachment organs of the monogenean Rajonchocotyle emarginata (Olsson, 1876) (Polyopisthocotylea: Hexabothriidae), a gill parasite of rays.

    abstract::The present study uses scanning and transmission electron microscopical observations to examine the haptoral features of the hexabothriid polyopisthocotylean monogenean Rajonchocotyle emarginata from the gills of the elasmobranch Amblyraja radiata. The haptor possesses six equal, pedunculate suckers, each armed with a...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-4973-x

    authors: Poddubnaya LG,Hemmingsen W,Gibson DI

    更新日期:2016-06-01 00:00:00

  • Return to sender: use of Plasmodium ER retrieval sequences to study protein transport in the infected erythrocyte and predict putative ER protein families.

    abstract::We have investigated how knowledge of endoplasmic reticulum (ER) retrieval signals can be used to study specific trafficking pathways in the malaria-infected erythrocyte. We show that addition of various lumenal ER retrieval signals to soluble green fluorescent protein (GFP) chimaera causes retrieval of the fusion pro...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-009-1397-x

    authors: Külzer S,Gehde N,Przyborski JM

    更新日期:2009-06-01 00:00:00

  • Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate.

    abstract::The present report describes a method for establishment of colonies of Blastocystis hominis from single cells in soft agar. The percentage of colony-forming efficiency (% CFE = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. Fiv...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050194

    authors: Tan SW,Singh M,Thong KT,Ho LC,Moe KT,Chen XQ,Ng GC,Yap EH

    更新日期:1996-01-01 00:00:00

  • Heat-shock and stress response of the parasitic nematode Haemonchus contortus.

    abstract::Before and after a stress treatment, larval stages and adult worms of Haemonchus contortus were tested for the induction and expression of heat-shock proteins (HSPs) using immunoblot analysis with HSP70-and HSP65-specific monoclonal antibodies. Stress treatment or heat shock did not alter the signals obtained with the...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00931852

    authors: van Leeuwen MA

    更新日期:1995-01-01 00:00:00

  • Caryospora cherrughi sp. n. (Apicomplexa: Eimeriidae) infecting Falco cherrug in Saudi Arabia.

    abstract::The oocysts of Caryospora cherrughi sp. n. were described from the feces of the saker falcon, Falco cherrug, collected from the falcon market in Riyadh City, Saudi Arabia. The prevalence of infection was 21.4 % (15/70). The majority of the oocysts examined had completed sporulation within 64-84 h at 24 ± 2 °C. Sporula...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-012-3217-y

    authors: Alfaleh FA,Alyousif MS,Al-Shawa YR,Al-Quraishy S

    更新日期:2013-03-01 00:00:00

  • Triton-labile antigens in flagella isolated from Giardia lamblia.

    abstract::Sheared flagella from Giardia lamblia were freed from cytoskeleton fragments and other cell contaminants by centrifuging in a density gradient. The purified organelles contain many polypeptides, including a set of low-molecular-weight antigens [apparent molecular weights (MWs) = 31, 32, 34, 35 and 37 kD] in the same s...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00535140

    authors: Clark JT,Holberton DV

    更新日期:1988-01-01 00:00:00

  • Immunosuppression in hamsters with progressive visceral leishmaniasis: an evaluation of the role of nitric oxide toward impairment of the lymphoproliferative response.

    abstract::The progressive visceral infection caused in golden hamsters by Leishmania donovani amastigotes led to gradual impairment of the proliferative response of their splenic (SPMC) or peripheral blood (PBMC) mononuclear cells to in vitro stimulation with leishmanial antigen, with mitogen (concanavalin A), and even with a c...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050600

    authors: Dasgupta S,Mookerjee A,Chowdhury SK,Ghose AC

    更新日期:1999-07-01 00:00:00

  • Occurrence and distribution of putative neurotransmitters in the frog-lung parasite Haplometra cylindracea (Trematoda: Digenea).

    abstract::The localisation and distribution of the cholinergic, serotoninergic and peptidergic components of the nervous system of the frog-lung fluke Haplometra cylindracea have been determined by the application of standard enzyme cytochemical and immunocytochemical techniques to cryostat sections and whole-mount preparations...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00931056

    authors: McKay DM,Halton DW,Johnston CF,Fairweather I,Shaw C

    更新日期:1990-01-01 00:00:00

  • Eimeria bovis infection enhances adhesion of peripheral blood mononuclear cells to and their transmigration through an infected bovine endothelial cell monolayer in vitro.

    abstract::The first schizogony of Eimeria bovis takes place in lymphatic endothelial cells of the ileum, resulting in the formation of macroschizonts within 2-3 weeks. In this study, we analyse early cellular immune responses to infected host cells on the basis of peripheral blood mononuclear cell (PBMC) adhesion on and transmi...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0517-8

    authors: Taubert A,Zahner H,Hermosilla C

    更新日期:2007-08-01 00:00:00