Abstract：
:The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave a significantly higher level of green fluorescence in Haemonchus contortus-resistant eggs as compared with that of susceptible eggs. In the same way, verapamilbodipy, a new fluorescent probe for the detection of multidrug resistance in cells, showed a significantly higher degree of binding to resistant eggs. The results confirm those obtained with biological drug assays using both anthelmintics and verapamil and provide a quantitative and effective methodology for the functional study of multidrug resistance in nematodes.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Kerboeuf D,Chambrier P,Le Vern Y,Aycardi Jdoi
10.1007/s004360050519subject
Has Abstractpub_date
1999-02-01 00:00:00pages
118-23issue
2eissn
0932-0113issn
1432-1955journal_volume
85pub_type
杂志文章abstract：:Spirometra erinacei is a pseudophyllidean tapeworm which inhabits the intestines of cats and dogs. The infections are usually asymptomatic in these animals, but the infection of the plerocercoid larvae of the parasite, spargana, cause sparganosis in other vertebrates, including human. In this study, we identified a ge...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1714-4
更新日期：2010-02-01 00:00:00
abstract：:The purpose of the present paper was to analyse the association between sequences of Entamoeba histolytica and their relationship with the development of hepatic abscesses in hamsters, using a complementary DNA library for E. histolytica. From the sequences obtained, we designed oligonucleotides for amplification by P...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0006-x
更新日期：2006-01-01 00:00:00
abstract：:Dimethyl sulfoxide (DMSO), commonly used for cryoprotection or for the solvation of cytoskeleton-modifying drugs, causes changes in the topology of the plasma membrane of the tegumental brush border in the tapeworm Hymenolepis diminuta; however, relatively long exposures of high concentrations are required. In tapewor...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00936184
更新日期：1992-01-01 00:00:00
abstract：:Flame atomic absorption spectrometry was performed to determine the alteration of calcium concentration in the soft parts and shells of Biomphalaria alexandrina and Bulinus truncatus due to the infection with Schistosoma mansoni and S. haematobium, respectively. The results showed significant lowering in the calcium c...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0566-z
更新日期：2007-09-01 00:00:00
abstract：:Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interac...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06887-x
更新日期：2020-11-01 00:00:00
abstract：:Telomerase, consisting of telomerase RNA and telomerase reverse transcriptase (TERT), is responsible for the maintenance of the end of linear chromosomes. TERT, as the catalytic subunit of telomerase, plays a critical role in telomerase activity. Researches indicate TERT-associated proteins participate in the regulati...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4108-1
更新日期：2014-10-01 00:00:00
abstract：:The Asian tiger mosquito Aedes albopictus has undergone a dramatic expansion of its range in the last few decades. Since its first detection in 2007 in Germany at the motorway A5 coming from Italy via Switzerland to Germany, it has been continuously introduced by vehicles, most probably from Italy. After a hint from a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5356-z
更新日期：2017-03-01 00:00:00
abstract：:The genomic DNA was extracted from nematode parasites, Heterakis gallinae from specific host, native chickens. The polymerase chain reaction (PCR) was applied to make amplification of the DNA content that aids in the further studies. The primer was used in the PCR reactions that have the following sequences: forward (...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3539-4
更新日期：2013-10-01 00:00:00
abstract：:This study aimed to investigate the links between parasites and behavioural traits of juvenile Murray cod (Maccullochella peelii). The Murray cod is an endangered Australian freshwater fish for which restocking programs are in place and there is a growing human consumption market. However, little is known about the pa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-07021-7
更新日期：2021-01-06 00:00:00
abstract：:The induction of protective immunity to Leishmania amazonensis was investigated by injection of parasite clones of low and medium virulence into susceptible mice. To this end, L. amazonensis were cloned by limiting dilution and the clones' virulence was evaluated by the course of infection in susceptible mice. Clones ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050321
更新日期：1997-01-01 00:00:00
abstract：:Pathogen-free rainbow trout (Oncorhynchus mykiss) aged 735 degree days were experimentally exposed to a low dose of infectious Myxobolus cerebralis (20 triactinomyxons fish(-1)). Three time periods were chosen for sampling that included 10 days (d), 67 d, and 5 months (mo) post exposure. Five diagnostic assays were us...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0166-3
更新日期：2006-09-01 00:00:00
abstract：:Neodiplostomum americanum Chandler and Rausch, 1947 has been reported from six species of owls in North America. At present, there are no molecular data for this species and gene sequence data from Neodiplostomum Railliet, 1919 are limited. A freshly deceased specimen of the Great Horned Owl Bubo virginianus Gmelin, 1...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5503-1
更新日期：2017-08-01 00:00:00
abstract：:Parasitological analyses are often based on invasive methodologies, involving host sacrifice, raising ethical and conservation issues. However, alternative non-invasive approaches may not be always applicable due to the location of the parasite in the host tissue or the quality and reliability of the non-invasive samp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3588-8
更新日期：2013-12-01 00:00:00
abstract：:Three monoclonal antibodies generated by immunization of mice with Plasmodium berghei-infected red blood cells were found to react with the 75-kDa heat-shock protein (HSP70) present in liver stages and erythrocytic forms of the parasites. These antibodies were shown to react with a recombinant protein encoding the car...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932618
更新日期：1994-01-01 00:00:00
abstract：:This report presents the results of the first faunistic study of hard ticks in Qazvin province of Iran. The primary objective was to determine the species diversity and geographic distribution of hard ticks that parasitize domestic ruminants. Information about the abiotic preferences of these species has been provided...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2501-6
更新日期：2012-01-01 00:00:00
abstract：:The morphology of the scolex of the tapeworm Silurotaenica siluri (Batsch, 1786) (Proteocephalidae: Gangesiinae), a specific parasite of European wels (Silurus glanis L.), was studied using light, scanning (SEM), and transmission electron (TEM) microscopy. The scolex of S. siluri is rather uniform in shape and size; i...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050498
更新日期：1999-01-01 00:00:00
abstract：:This study increases the known biodiversity of cnidarian parasites in neotropical bryconid fishes. Two novel Myxobolus species are described based on morphology, ultrastructure and small subunit ribosomal DNA (ssrDNA) sequencing: Myxobolus vetuschicanus n. sp. infecting fins of Salminus franciscanus and Myxobolus mine...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06863-5
更新日期：2020-11-01 00:00:00
abstract：:Little information is available on the occurrence of endoparasites in pet guinea pigs (Cavia porcellus). The purpose of this study was to evaluate the prevalence of intestinal parasites in cavies kept as pets in southern Italy. Fresh fecal samples were randomly collected from 60 guinea pigs housed in pet shops or priv...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4289-7
更新日期：2015-03-01 00:00:00
abstract：:The complex life cycle of Toxoplasma gondii involves many animal species, raising zoonotic, economic, and conservation issues. This complexity is reflected in the molecular structure of T. gondii, whose different genotypes differ in pathogenicity. Among the intermediate hosts of T. gondii, wild ungulates may be a sour...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4981-x
更新日期：2016-05-01 00:00:00
abstract：:Mosquitoes transmit dreadful diseases to human beings wherein biological control of these vectors using plant-derived molecules would be an alternative to reduce mosquito population. Aqueous leaf extract and green synthesized silver nanoparticles (Ag NPs) from Pedilanthus tithymaloides (L.) Poit. were investigated for...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3138-9
更新日期：2013-01-01 00:00:00
abstract：:This study assessed the anisakid nematode distribution pattern in the fish collected from coasts of Mediterranean Sea, Egypt, during the period September 2010-April 2011. Two hundred thirty out of 300 (76.7%) Dicentrarchus labrax (European seabass) marine fishes belonging to family Moronidae were dissected and found t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2600-4
更新日期：2012-03-01 00:00:00
abstract：:The protozoan parasite Giardia duodenalis is known to infect humans and a wide range of animals globally. However, no studies on G. duodenalis infection in Bactrian camels have been reported. In the present study, in order to examine the prevalence and genetic diversity of G. duodenalis in Bactrian camels, 852 fecal s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06905-y
更新日期：2020-11-01 00:00:00
abstract：:Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050568
更新日期：1999-05-01 00:00:00
abstract：:Lungworms of the genera Parafilaroides and Otostrongylus are responsible for parasitic bronchopneumonia, the foremost disease of eastern Atlantic common seals (EACS, Phoca vitulina vitulina) in the Dutch North Sea. Recently, there have been increased reports of lungworm cases and observations of unusually long Parafil...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06675-7
更新日期：2020-06-01 00:00:00
abstract：:Trypanosoma cruzi causes Chagas' disease, a systemic infection that affects cells of meso-, endo-, and ectodermic origin. However, as far as we know, the presence of T. cruzi stages in bone has not been reported previously, and it has scarcely been investigated in cartilage. We inoculated 7- and 20-day-old (8 and 15 g...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0211-2
更新日期：2006-11-01 00:00:00
abstract：:Sarcophagids are a large family of Diptera, with a worldwide distribution. They are related to decomposing organic matter and are very interesting for health science and in forensic cases since many species produce myiasis and occur in human corpses. This family is considered difficult to study, particularly with rega...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4631-8
更新日期：2015-11-01 00:00:00
abstract：:A detailed study of the surface topography of the echinostome digenean Mesorchis denticulatus (Rudolphi 1802) Dietz 1909 was carried out using the scanning electron microscope. The surface area of the redia is increased by microvilli, and ciliate sensory structures are common around the mouth. The cannibalistic behavi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00536336
更新日期：1987-01-01 00:00:00
abstract：:A total of 70 proven cases of neurocysticercosis from la Réunion (Indian Ocean) were studied with enzyme-linked immunoassay (ELISA) and immunoelectrotransfer blot (EITB) to detect specific antibodies in serum and cerebrospinal fluid (CSF). Absorbance levels of antibody to crude Taenia solium cyst extract as an antigen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931618
更新日期：1995-01-01 00:00:00
abstract：:Prior studies have shown that filarial nematodes can effectively metabolize hydrogen peroxide in excess of that generated by activated host cells. However, the mechanisms of H2O2 removal by the filarial parasites are unclear. Herein we report the results of studies carried out on the biochemical activity and on immuno...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050032
更新日期：2000-03-01 00:00:00
abstract：:The neuromuscular system (NMS) in cercariae of Neoastiotrema trituri, Plagiorchis elegans, Omphalometra flexuosa, Skrjabinoeces similis and Prosthogonimus ovatus was studied with immunocytochemical methods and confocal scanning laser microscopy. The patterns of F-actin in the musculature, 5-HT immunoreactive (IR), FMR...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3044-1
更新日期：2012-11-01 00:00:00
