Abstract：
:Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi organisms were continuously cultivated at 37 degrees C in microaerophilous stationary-phase culture in a humidified atmosphere containing 5% CO2 in air before exposure to serum-free culture conditions. For serum-free propagation, lipid-rich bovine serum albumin (LR-BSA), alone or with chemically defined lipids (CDL), were added instead of serum. Media containing LR-BSA alone or LR-BSA and CDL in various amounts supported the in vitro propagation of B. caballi. Growth was maintained for more than 6 months. The growth rates obtained in serum-free media were similar to those previously obtained in traditional serum-containing medium.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Zweygarth E,van Niekerk CJ,de Waal DTdoi
10.1007/s004360050568subject
Has Abstractpub_date
1999-05-01 00:00:00pages
413-6issue
5eissn
0932-0113issn
1432-1955journal_volume
85pub_type
杂志文章abstract：:The larvicidal activities of methanolic fractions from Adhatoda vasica leaf extracts were investigated against the bancroftian filariasis vector Culex quinquefasciatus and dengue vector Aedes aegypti. The results indicated that the mortality rates was high at 100, 150, 200 and 250 ppm of methanol extract of fractions ...
journal_title：Parasitology research
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doi：10.1007/s00436-011-2728-2
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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pub_type： 杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
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