DNA extraction from Ascaris suum muscle tissue.

Abstract:

:A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Di Mito C,Betschart B

doi

10.1007/s004360050456

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

596-7

issue

7

eissn

0932-0113

issn

1432-1955

journal_volume

84

pub_type

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    pub_type: 杂志文章

    doi:10.1007/BF00931901

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