Abstract:
:A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Di Mito C,Betschart Bdoi
10.1007/s004360050456subject
Has Abstractpub_date
1998-07-01 00:00:00pages
596-7issue
7eissn
0932-0113issn
1432-1955journal_volume
84pub_type
杂志文章abstract::The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0165-4
更新日期:2006-07-01 00:00:00
abstract::The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence al...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-005-0036-4
更新日期:2006-04-01 00:00:00
abstract::Infections of Eimeria tenella in chicken embryos were used to compare the anticoccidial activity of ten drugs. The minimal inhibitory concentration (MIC) and minimal toxic concentration (MTC) were affected by the time of inoculation into the embryos and by the chemical nature of the compounds. Some compounds (nicarbaz...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931020
更新日期:1991-01-01 00:00:00
abstract::A comprehensive icthyoparasotological survey among estuarine and freshwater fishes of West Bengal, India revealed Trichodinella sunderbanensis sp. nov. and Trichodina acuta Lom, 1961 from an estuarine fish Mystus gulio (Hamilton, 1822) from the Vidyadhari river; Trichodina nandusi sp. nov. from a freshwater fish, Nand...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-3234-x
更新日期:2013-03-01 00:00:00
abstract::Human cystic and alveolar echinococcoses are zoonotic diseases caused by the larval stages of Echinococcus granulosus and Echinococcus multilocularis, respectively. As the diseases are co-endemic in many areas of the world, a simple and rapid test for the differential diagnosis of cystic echinococcosis (CE) and alveol...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3550-9
更新日期:2013-10-01 00:00:00
abstract::The hypothesis of a positive correlation between host specificity and taxonomic diversification was tested in a family of fish ectoparasites, the Diplectanidae Bychowsky 1957 (Monogenea). A comparative analysis of correlation of species richness with host specificity was performed using an adapted independent contrast...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360000325
更新日期:2001-03-01 00:00:00
abstract::Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of plant origin my serve as an alternative biocontrol technique in the future. The aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extra...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4856-6
更新日期:2016-03-01 00:00:00
abstract::For the identification of host species of blood-sucking parasites, the suitability of disc-electrophoresis of the stomach contents was tested. Mammalian blood in the stomach of the medicinal blood leech Hirudo medicinalis gave satisfactory results. In the case of mixed blood samples from H. medicinalis, the identifica...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931901
更新日期:1993-01-01 00:00:00
abstract::The tegument proteins of schistosomes are regarded as potential vaccine candidates and drug targets to control schistosomiasis. Nucleotide pyrophosphatase/phosphodiesterase-5 (NPP-5), which belongs to a multigene family of nucleotide pyrophosphatase/phosphodiesterases (NPPs), is important in the hydrolysis of pyrophos...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2552-8
更新日期:2012-02-01 00:00:00
abstract::Common frogs (Rana temporaria) were exposed either to third-stage larvae (L3) or to first-stage larvae (L1) of Angiostrongylus vasorum. Following exposure to L3, viable larvae could be detected in the frogs for at least 2 weeks. Following exposure to L1, the frogs developed viable L3 in their tissues within 30 days. I...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931834
更新日期:1993-01-01 00:00:00
abstract::Precise identification of avian schistosomes in the genus Trichobilharzia at the species level is difficult and requires both traditional morphological and molecular techniques. To obtain satisfactory results by traditional methods, the characteristics of the intact adults or large fragments of male and females are ne...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06573-7
更新日期:2020-03-01 00:00:00
abstract::The narrow Andean-Patagonian temperate rainforest strip in the west of southern South America is inhabited by two endemic species of cervids, the southern pudu (Pudu puda) and the huemul (Hippocamelus bisulcus), both cataloged as near threatened and threatened species, respectively. One of the possible causes of their...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5429-7
更新日期:2017-05-01 00:00:00
abstract::The value profiles of 5 intracellular enzymes, 15 metabolites (with 2 associated ratios), and 3 electrolytes were monitored over time in 9 captive-reared African black-footed penguins (Spheniscus demersus) with different avian malaria clinical status: uninfected, subclinically infected, and clinically infected with fa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931537
更新日期:1995-01-01 00:00:00
abstract::The effects of a live Cryptobia salmositica (Kinetoplastida) vaccine on the humoral and cellular immune response, and changes in the peripheral leukocyte populations of Salmo salar were investigated. The vaccine produced detectable parasitemia in the blood which peaked at 5 weeks post-vaccination (w.p.v). Antibodies w...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1270-x
更新日期:2005-03-01 00:00:00
abstract::Two hundred forty feral domestic cats trapped between 2004 and 2008 in the city centre and the suburb districts of Dubai, as well as in desert biotopes, were subjected to a complete parasitological dissection. The established parasite fauna consisted of Cystoisospora felis (12.9%), Cystoisospora rivolta (9.2%), Toxopl...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1372-6
更新日期:2009-07-01 00:00:00
abstract::Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with st...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1138-6
更新日期:2008-11-01 00:00:00
abstract::Intestinal coccidiosis of rabbits induced by E. coecicola causes enormous economic losses in rabbit farms. Here, we investigate the effect of E. coecicola on the liver of the rabbit Oryctolagus cuniculus. On day 7 p.i., fecal expulsion of E. coecicola oocysts is maximal and rabbits have lost approximately 25% of their...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2574-2
更新日期:2012-02-01 00:00:00
abstract::Three in vitro studies were designed to develop an assay for anticoccidial efficacy by use of laboratory (Houghton) and field (T-376) Eimeria tenella strains. In study (1), minimum inhibitory concentrations (MICs) of monensin (Mon), maduramicin (Mad), salinomycin (Sal), and lasalocid (Las) were determined that are abl...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4404-4
更新日期:2015-06-01 00:00:00
abstract::Sarcocysts were detected in routinely processed histological sections of skeletal muscle, but not cardiac muscle, of two adult male otters (Lutra lutra; Mustelidae) from northern Norway following their post-mortem examination in 1999 and 2000. The sarcocysts were slender, spindle-shaped, up to 970 μm long and 35-70 μm...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4251-8
更新日期:2015-03-01 00:00:00
abstract::The recent resurgence of the common bed bug Cimex lectularius L. throughout western industrialized nations has been facilitated in part by the insect becoming pesticide-resistant. Novel control strategies, including xenointoxication, should be considered to combat C. lectularius. Ivermectin, a U.S. Food and Drug Admin...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5062-x
更新日期:2016-08-01 00:00:00
abstract::Cytokine responses of lymphocytes against Toxoplasma gondii are usually studied by stimulating the cells from infected animals with tachyzoite lysate antigens (TLA) in vitro. We examined whether strains (genotypes) of the parasite used for TLA affect the production of IL-2, IL-4, IL-10 and IFN-gamma in the culture of ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-005-1416-5
更新日期:2005-10-01 00:00:00
abstract::Gonadectomized male laboratory rats were given 0.06 mg/kg estradiol benzoate daily for 14 days before being inoculated with 50 third-stage larvae of Parastrongylus malaysiensis. Hormone treatment was continued until the rats were killed. The numbers of larvae in the brain and of adult worms in the pulmonary area of th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932628
更新日期:1994-01-01 00:00:00
abstract::The first schizogony of Eimeria bovis takes place in lymphatic endothelial cells of the ileum, resulting in the formation of macroschizonts within 2-3 weeks. In this study, we analyse early cellular immune responses to infected host cells on the basis of peripheral blood mononuclear cell (PBMC) adhesion on and transmi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0517-8
更新日期:2007-08-01 00:00:00
abstract::Light, electron microscopy and DNA analyses were performed to characterize a microsporidium infecting Rachiplusia nu larvae from a laboratory rearing in Argentina. Diplokaryotic spores were oval and measured 3.61 ± 0.29 × 1.61 ± 0.14 μM (fresh). The spore wall was composed of an electron-dense exospore and an electron...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5820-z
更新日期:2018-05-01 00:00:00
abstract::The malaria parasite, Plasmodium falciparum, invades the human erythrocyte through a complex interaction with erythrocyte receptors characterized by patterns of resistance to various enzymes. As invasion rates are influenced by blood group polymorphisms, we reasoned that the extremely rare rhesus null (Rh(null)) eryth...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0756-8
更新日期:2008-01-01 00:00:00
abstract::During the period from January to December (2007), 1,095 freshwater molluscs of four species were captured (Lymnaea stagnalis, Stagnicola elodes, Physella cubensis and Physa acuta) in Lake Chicnahuapan, State of Mexico, Mexico. Two hundred seventy-two (24.84% prevalence) of these molluscs were parasitised by 11 tremat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1536-4
更新日期:2009-10-01 00:00:00
abstract::A substitution (Phe-->Ile) within the sodium channel gene sequence has been associated with pyrethroid resistance in Boophilus microplus. The aim of the present study was to analyze the inheritance of pyrethroid resistance and the mutant allele, on reciprocal crosses of a susceptible (SS) and a resistant (RR) strain. ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1024-2
更新日期:2008-08-01 00:00:00
abstract::As Cryptosporidium parvum and Giardia lamblia can be disseminated in the environment by avian hosts, a total of 499 fecal dropping from 308 free-ranging, 90 captive, and 101 domestic birds were tested by conventional, immunological, and molecular techniques for these human enteropathogens. Twenty-six (5.2%) tested pos...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1293-9
更新日期:2009-04-01 00:00:00
abstract::Light, scanning and transmission electron microscope examinations of adult Cystidicoloides ephemeridarum nematodes, parasitic in the stomach of brown trout (Salmo trutta fario L.), revealed that the body wall is composed of a cuticle, a hypodermis and longitudinally oriented somatic musculature. The body cuticle is co...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-0935-1
更新日期:2003-09-01 00:00:00
abstract::Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. A complete open reading frame of oligopeptidase B from Leishmania amazonensis was amplified with polymerase chain reaction with gradient annealing temperatures using primers designed...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0552-5
更新日期:2007-09-01 00:00:00