Abstract：
:Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. A complete open reading frame of oligopeptidase B from Leishmania amazonensis was amplified with polymerase chain reaction with gradient annealing temperatures using primers designed for the oligopeptidase B gene from L. major. The 2,196-bp fragment coded for a protein of 731 amino acids with a predicted molecular mass of 83.49 KDa. The encoded protein (La_OpB) shares a 90% identity with oligopeptidases of L. major and L. infantum, 84% with L. braziliensis, and approximately 62 identity with Trypanosoma peptidases. The oligopeptidase B gene is expressed in all cycle stages of L. amazonensis. The three dimensional model of La_OpB was obtained by homology modeling based on the structure of prolyl oligopeptidases. We mapped a La_OpB model that presents a greater negative charge than prolyl oligopeptidases; our results suggest a difference in the S2 subsite when compared to oligopeptidases B from Trypanosoma and bacterial oligopeptidases B. The La_OpB model serves as a starting point for its exploration as a potential target source for a rational chemotherapy.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
de Matos Guedes HL,Carneiro MP,Gomes DC,Rossi-Bergmanmn B,Giovanni de Simone Sdoi
10.1007/s00436-007-0552-5subject
Has Abstractpub_date
2007-09-01 00:00:00pages
853-63issue
4eissn
0932-0113issn
1432-1955journal_volume
101pub_type
杂志文章abstract：:Capillaria hepatica (syn. Calodium hepaticum) is a globally distributed nematode with a high affinity to the liver of a wide range of mammalian hosts, including humans. Documented reports of the nematode in cats and associated histopathology are rare. Here, we describe a case of C. hepatica infection in a 5-year-old m...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-021-07056-4
更新日期：2021-01-22 00:00:00
abstract：:Blastocytis sp. is the most common enteric protozoan in human, but its pathogenesis is still unclear. To study the infectious effects of Blastocytis sp. on tissue damage, we orally challenged the Sprague Dawley (SD) rats with different doses of Blastocytis subtype1 (ST1) and examined the histological changes. We found...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3536-7
更新日期：2013-10-01 00:00:00
abstract：:This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0851-x
更新日期：2008-04-01 00:00:00
abstract：:The effects of trypsin, bile, trypsin-bile, pepsin, dithiothreitol (DTT) and metacercarial excretory-secretory product (ESP) on the in vitro excystment of Clonorchis sinensis metacercariae were investigated. The majority of metacercariae excysted immediately in trypsin-bile in PBS solution, a process which was complet...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1097-5
更新日期：2004-05-01 00:00:00
abstract：:Recent studies have shown that hormones could induce anti-parasitic functions of the host immune system; thus, the aim of the present study was to estimate the seroprevalence of Toxoplasma gondii antibodies by an enzyme-linked immunosorbent assay in a Polish population of women and men with hyperprolactinaemia (n = 23...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0824-0
更新日期：2008-03-01 00:00:00
abstract：:Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic mi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0763-8
更新日期：2003-06-01 00:00:00
abstract：:Phalloidin fluorescence technique, enzyme cytochemistry and immunocytochemistry in conjunction with confocal scanning laser microscopy have been used for the first time to describe the nervous and muscle systems of the viviparous monogenean gill parasite, Macrogyrodactylus clarii. The gross spatial arrangement of musc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1198-1
更新日期：2004-10-01 00:00:00
abstract：:Domestic cats and other felids are definitive hosts for the zoonotic protozoan parasite Toxoplasma gondii. Serology is widely used in epidemiological studies conducted to estimate the proportion of domestic cats that have encountered the parasite. However, a limited number of such studies are available from some regio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06368-w
更新日期：2019-08-01 00:00:00
abstract：:In a survey study on the macroscopic species of Sarcocystis infecting domestic sheep (Ovis aries) and cattle (Bos taurus) in Egypt, the macrosarcocysts of Sarcocystis gigantea and Sarcocystis medusiformis were detected in the carcasses of 33 domestic sheep out of a total of 250 (13.20%), whereas Sarcocystis hirsuta ma...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-07002-w
更新日期：2021-01-18 00:00:00
abstract：:Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocystis ratti n. sp. Und...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06393-9
更新日期：2019-09-01 00:00:00
abstract：:As Cryptosporidium parvum and Giardia lamblia can be disseminated in the environment by avian hosts, a total of 499 fecal dropping from 308 free-ranging, 90 captive, and 101 domestic birds were tested by conventional, immunological, and molecular techniques for these human enteropathogens. Twenty-six (5.2%) tested pos...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1293-9
更新日期：2009-04-01 00:00:00
abstract：:Male Sprague-Dawley rats were dosed orally with nitroxynil at a concentration of 40 mg/kg and adult Fasciola hepatica recovered after 24 h, 48 h and 72 h. Surface changes to the flukes were monitored by means of SEM. After the 24 h treatment, extensive swelling and blebbing of the tegument was observed on both surface...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-0930-6
更新日期：2003-10-01 00:00:00
abstract：:Triatoma platensis is occasionally found coexisting with Triatoma infestans in chicken coops in Argentina. Some authors have reported the presence of hybrid specimens of both species in chicken coops and other peridomestic habitats. Given the coexistence of T. infestans with T. platensis and the possibility of generat...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06822-0
更新日期：2020-09-01 00:00:00
abstract：:The development of Babesia equi was studied in the salivary glands of adult female ticks, Boophilus microplus, using a transmission electron microscope (TEM). Engorged nymphs were obtained from splenectomized foals experimentally infected with B. equi and fed in the adult phase for 5 days on rabbits. Sporogony in B. e...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050359
更新日期：1998-01-01 00:00:00
abstract：:In an effort to identify novel therapeutic alternatives for the treatment of malaria, the present study evaluated the antimalarial effect of the crude hydroalcoholic extract (HCE) from the leaves of Chenopodium ambrosioides L. For this purpose, the molecular affinity between the total proteins from erythrocytes infect...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5216-x
更新日期：2016-11-01 00:00:00
abstract：:Although the life cycle of the equid stomach parasite Habronema muscae was disclosed more than 100 years ago, little is known about the effect of the developing nematode larvae in its intermediate host, Musca domestica. In a series of experiments, freshly hatched M. domestica larvae were exposed to H. muscae eggs cont...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5312-y
更新日期：2017-02-01 00:00:00
abstract：:The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06796-z
更新日期：2020-09-01 00:00:00
abstract：:Human hydatidosis is characterized by a prolonged coexistence of Echinococcus granulosus and its host without effective rejection of the parasite. This parasitic infection constitutes a major health problem in Algeria. In this study, we investigated in vivo production of nitrite (NO(2)(-) + NO(3)(-)) in sera of Algeri...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3181-6
更新日期：2013-02-01 00:00:00
abstract：:The complex life cycle of Toxoplasma gondii involves many animal species, raising zoonotic, economic, and conservation issues. This complexity is reflected in the molecular structure of T. gondii, whose different genotypes differ in pathogenicity. Among the intermediate hosts of T. gondii, wild ungulates may be a sour...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4981-x
更新日期：2016-05-01 00:00:00
abstract：:Toxoplasma gondii uses a unique mechanism to fulfill its asexual life cycles by which the parasite can infect all the warm-blooded animals including humans. Mitogen-activated protein kinase (MAPK) or extracellular signal-regulated kinase (ERK) pathway widely existed in eukaryotic cells mediates the conversion of envir...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5103-5
更新日期：2016-09-01 00:00:00
abstract：:Avian blood parasites have been shown to have significant health effects on avifauna worldwide. Sri Lanka, a tropical island rich with resident and migratory birds, has not been properly evaluated for avian blood parasites or their vectors. We investigated the presence of avian haemoparasites in Sri Lankan birds and t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-021-07049-3
更新日期：2021-01-16 00:00:00
abstract：:The migratory response of peripheral blood granulocytes and monocytes from the European eel Anguilla anguilla (L.) to infective larvae of the swimbladder nematode Anguillicola crassus Kuwahara, Niimi and Hagaki, 1974 was examined by means of light microscopical histology and with an in vitro assay using a modified Boy...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-0910-y
更新日期：2008-05-01 00:00:00
abstract：:The infectivity of Cryptosporidium muris (strain RN 66), originally isolated from the house rat (Iseki 1986), to various laboratory animals was studied by transmission experiments. After oral inoculation with 1 x 10(6) oocysts, mice, guinea pigs, rabbits, dogs, and cats all discharged endogenously produced oocysts in ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931279
更新日期：1989-01-01 00:00:00
abstract：:The nematode Anisakis simplex is a marine parasite that causes allergy as well as anisakiasis. Although five Anisakis allergens have already been identified, immunoblotting studies suggested that unidentified allergens still exist. In this study, an expression cDNA library constructed from A. simplex was subjected to ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0396-4
更新日期：2007-05-01 00:00:00
abstract：:It is well known that toxoplasmosis can be life threatening to immunocompromised individuals such as AIDS and organ transplantation patients. Glucocorticoids (GCs) are widely used in the clinic for the treatment of autoimmune diseases and organ transplantation resulting in acute toxoplasmosis in these patients. Howeve...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3661-3
更新日期：2014-01-01 00:00:00
abstract：:The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050519
更新日期：1999-02-01 00:00:00
abstract：:An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h. ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050446
更新日期：1998-07-01 00:00:00
abstract：:Albendazole is known as the drug of choice for medical treatment of cystic echinococcosis (CE). Albendazole sulfoxide (ABZ-SO), as the main active metabolite of albendazole, has low efficacy in the disease due to low water solubility and poor absorptivity. PLGA nanoparticles (NPs) enhance the dissolution of poorly sol...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06901-2
更新日期：2020-12-01 00:00:00
abstract：:The development of redial burden and cercarial shedding were studied in two groups of Lymnaea truncatula subjected to successive cross-exposures to one miracidium of Paramphistomum daubneyi and one of Fasciola hepatica per snail, or vice versa. The results were compared with those obtained in controls subjected to two...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050175
更新日期：1996-01-01 00:00:00
abstract：:The parasite community structure of the neotropical cormorant, Phalacrocorax brasilianus, from two lagoons (Coyuca and Tres Palos) from Guerrero state, México, was examined. Fourteen species of adult helminths (6,391 individuals) from 48 cormorants were identified: 9 digeneans, 1 acanthocephalan, 1 cestode, and 3 nema...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2377-5
更新日期：2011-11-01 00:00:00
