Abstract：
:Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic migration patterns, isoelectric point determination and Western blot studies failed to reveal other EhCP molecules. Polyclonal antibodies against the purified EhCP2 prepared in rabbits either stabilized or enhanced the enzyme activity in a dose-response manner. Purified EhCP2 was enclosed within inert resin microspheres (22-44 microm in diameter) and injected into the portal vein of normal hamsters. In the liver, the microspheres caused mild acute inflammation and occasional minimal necrosis of short duration. Sections of the liver were immunohistochemically stained with the anti-EhCP2 antibody and the microspheres were positive for only a very short period (1 h) after injection. Sections of experimental acute (1 day, 5 days) amebic liver abscess produced in hamsters were also stained with the anti-EhCP2 antibody; and amebas were intensely positive but no staining was observed at any time in the surrounding necrotic structures. It is suggested that EhCP2 plays either a minor or no role in the causation of tissue damage in experimental acute liver amebiasis.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Olivos-García A,González-Canto A,López-Vancell R,García de León Mdel C,Tello E,Nequiz-Avendaño M,Montfort I,Pérez-Tamayo Rdoi
10.1007/s00436-002-0763-8subject
Has Abstractpub_date
2003-06-01 00:00:00pages
212-20issue
3eissn
0932-0113issn
1432-1955journal_volume
90pub_type
杂志文章abstract：:High levels of lactate dehydrogenase (LDH; EC 1. 1. 1. 27) activity have been detected in the filarial worm Molinema dessetae. The two major LDH isoenzymes (LDH1 and LDH2) from female worms were purified by successive chromatography on diethylaminoethyl (DEAE)-Sepharose, carboxymethyl (CM)-Sepharose, and hydroxyapatit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050184
更新日期：1996-01-01 00:00:00
abstract：:Ingestion of the larval nematode Angiostrongylus cantonensis can cause the human eosinophilic meningitis known as angiostrongyliasis. Analysis of the extracts and excretory-secretory (ES) products of A. cantonensis larvae and adult stages on gelatin substrate zymography demonstrated the presence of distinct gelatinoly...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1484-6
更新日期：2005-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 临床试验,杂志文章
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abstract：:Mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. Therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. Competent vectors of dengue and chikungunya viz. Aedes aegypti and Aedes albo...
journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0509-8
更新日期：2007-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5255-3
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931280
更新日期：1989-01-01 00:00:00
abstract：:This study evaluated, for the first time, the genetic diversity of Toxoplasma gondii isolates from free-range chickens from the state of Paraíba, Northeast Brazil. Tissue samples from 33 chickens from properties in five municipalities of Paraíba (Esperança, Olho d'Água, Malta, Monteiro, and Patos) were bioassayed in m...
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更新日期：2017-08-01 00:00:00
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更新日期：2019-09-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2012-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5031-4
更新日期：2016-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050359
更新日期：1998-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Parasitology research
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更新日期：2017-03-01 00:00:00
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更新日期：2013-04-01 00:00:00
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更新日期：2019-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2002-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2014-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050409
更新日期：1998-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2018-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
abstract：:A majority of Kudoa spp. infects the somatic muscle of fish establishing cysts. Previously, elevated humoral responses were detected in BALB/c mice immunised with Kudoa sp. pseudocyst extracts and in BALB/c mice orally inoculated with Kudoa sp. pseudocysts, as well as the presence of anti-Kudoa sp. antibodies in human...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1017-1
更新日期：2008-08-01 00:00:00
