Amebic cysteine proteinase 2 (EhCP2) plays either a minor or no role in tissue damage in acute experimental amebic liver abscess in hamsters.


:Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic migration patterns, isoelectric point determination and Western blot studies failed to reveal other EhCP molecules. Polyclonal antibodies against the purified EhCP2 prepared in rabbits either stabilized or enhanced the enzyme activity in a dose-response manner. Purified EhCP2 was enclosed within inert resin microspheres (22-44 microm in diameter) and injected into the portal vein of normal hamsters. In the liver, the microspheres caused mild acute inflammation and occasional minimal necrosis of short duration. Sections of the liver were immunohistochemically stained with the anti-EhCP2 antibody and the microspheres were positive for only a very short period (1 h) after injection. Sections of experimental acute (1 day, 5 days) amebic liver abscess produced in hamsters were also stained with the anti-EhCP2 antibody; and amebas were intensely positive but no staining was observed at any time in the surrounding necrotic structures. It is suggested that EhCP2 plays either a minor or no role in the causation of tissue damage in experimental acute liver amebiasis.


Parasitol Res


Parasitology research


Olivos-García A,González-Canto A,López-Vancell R,García de León Mdel C,Tello E,Nequiz-Avendaño M,Montfort I,Pérez-Tamayo R




Has Abstract


2003-06-01 00:00:00












  • Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore.

    abstract::Blastocystis is an enteric protozoan parasite commonly found in humans and animals. Phylogenetic and genotypic analyses have shown that Blastocystis exhibits extreme genetic diversity, and humans are host to a number of zoonotic isolates. In the present study, the prevalence of Blastocystis in 276 stool samples from a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wong KH,Ng GC,Lin RT,Yoshikawa H,Taylor MB,Tan KS

    更新日期:2008-03-01 00:00:00

  • Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis.

    abstract::Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 an...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ribeiro Vda S,Nunes Dda S,Gonzaga HT,da Cunha-Junior JP,Costa-Cruz JM

    更新日期:2014-07-01 00:00:00

  • Inhibitory effects of novel ciprofloxacin derivatives on the growth of four Babesia species and Theileria equi.

    abstract::The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Batiha GE,Tayebwa DS,Beshbishy AM,N'Da DD,Yokoyama N,Igarashi I

    更新日期:2020-09-01 00:00:00

  • Sarcocystis oreamni, n. sp. (Apicomplexa: Sarcocystidae) from the mountain goat (Oreamnos americanus).

    abstract::Numerous species of Sarcocystis have been reported from wild ruminants, but none has been named from the Rocky Mountain goat (Oreamnos americanus). Mature sarcocysts were found in frozen muscle samples of three of seven mountain goats from Alaska, USA. Two morphological types of sarcocysts were found; one had Sarcocys...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Calero-Bernal R,Van Wilpe E,White K,Verma SK,Cerqueira-Cézar CK,Dubey JP

    更新日期:2015-11-01 00:00:00

  • Genetic regulation of arrested development in nematodes: are age-1 and daf-gene orthologs present in Dictyocaulus viviparus?

    abstract::In opposite to the free-living soil nematode Caenorhabditis elegans, the genetic regulation of hypobiosis or inhibited or arrested development in parasitic nematodes is completely unknown. In C. elegans, the daf-genes or the age-1 gene are of major importance in signaling pathways regulating arrested development. To i...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Strube C,von Samson-Himmelstjerna G,Schnieder T

    更新日期:2007-09-01 00:00:00

  • The new national integrated strategy emphasizing infection sources control for schistosomiasis control in China has made remarkable achievements.

    abstract::Schistosomiasis japonica remains one important public health concern that cause great loss of humans' health and social-economic development in the Peoples' Republic of China. At the end of 1990s and the beginning of 2000s, there were still about 0.8 million patients and nearly 85 million people living in the epidemic...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu R,Dong HF,Jiang MS

    更新日期:2013-04-01 00:00:00

  • Eimeria bovis infection enhances adhesion of peripheral blood mononuclear cells to and their transmigration through an infected bovine endothelial cell monolayer in vitro.

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    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Taubert A,Zahner H,Hermosilla C

    更新日期:2007-08-01 00:00:00

  • Dose- and time-dependent functional and structural damage to the colon mucosa by Entamoeba histolytica trophozoite lysates.

    abstract::Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lys...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Navarro-García F,López-Revilla R,Tstusumi V

    更新日期:1993-01-01 00:00:00

  • The metacercariae of sibling species of Meiogymnophallus, including M. rebecqui comb. nov. (Digenea: Gymnophallidae), and their effects on closely related Cerastoderma host species (Mollusca: bivalvia).

    abstract::The morphology and ecology are described for four sibling species of digenean metacercariae: Meiogymnophallus minutus (Cobbold 1859), M. strigatus (Lebour 1908) from the estuarine cockle Cerastoderma edule L. and M. fossarum (Bartoli, 1965) and M. rebecqui (Bartoli, 1983) comb. nov. from the brackish water lagoon cock...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bowers EA,Bartoli P,Russell-Pinto F,James BL

    更新日期:1996-01-01 00:00:00

  • First report of typical Brazilian Toxoplasma gondii genotypes from isolates of free-range chickens (Gallus gallus domesticus) circulating in the state of Paraíba, Northeast Brazil.

    abstract::This study evaluated, for the first time, the genetic diversity of Toxoplasma gondii isolates from free-range chickens from the state of Paraíba, Northeast Brazil. Tissue samples from 33 chickens from properties in five municipalities of Paraíba (Esperança, Olho d'Água, Malta, Monteiro, and Patos) were bioassayed in m...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Feitosa TF,Vilela VLR,de Almeida-Neto JL,de Melo LRB,de Morais DF,Alves BF,Nakashima F,Gennari SM,Athayde ACR,de Jesus Pena HF

    更新日期:2017-08-01 00:00:00

  • Protein characterization of Babesia equi piroplasms isolated from infected horse erythrocytes.

    abstract::Proteins of Babesia equi piroplasms were characterized. The piroplasms of B. equi were purified by lysis of infected horse erythrocytes with N2 gas cavitation followed by separation in Percoll density-gradient centrifugation. The relative molecular weights (Mr) of major proteins separated by two-dimensional sodium dod...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ali S,Sugimoto C,Matsuda M,Sugiura T,Kanemaru T,Onuma M,Kamada M

    更新日期:1993-01-01 00:00:00

  • Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis.

    abstract::Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukar...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu MM,Yuan ZG,Peng GH,Zhou DH,He XH,Yan C,Yin CC,He Y,Lin RQ,Song HQ,Zhu XQ

    更新日期:2010-04-01 00:00:00

  • The precocious oncomiracidium of Neoentobdella parvitesticulata (Monogenea: Capsalidae: Entobdellinae).

    abstract::The oncomiracidium (larva) of the entobdelline Neoentobdella parvitesticulata (Monogenea: Capsalidae), adults of which parasitise the skin of the stingray Himantura fai (Dasyatidae) at Heron Island, Great Barrier Reef, Queensland, Australia, is described. The larva undergoes precocious development (progenesis) in the ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Whittington ID,Kearn GC

    更新日期:2005-07-01 00:00:00

  • Protective immunity against Toxoplasma gondii in mice induced by the SAG2 internal image of anti-idiotype antibody.

    abstract::A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 inte...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Yang CD,Chang GN,Chao D

    更新日期:2003-12-01 00:00:00

  • Seasonal dynamics of Trichodina spp. on whiting (Merlangius merlangus)in relation to organic pollution on the eastern Black Sea coast of Turkey.

    abstract::It was determined that there is a relationship between prevalence and mean densities of Trichodina spp. on Merlangius merlangus and organic pollution, measured as levels of nitrite, nitrate and phosphate in the surrounding environment. Monthly, two left anterior gill arcs of 60 M. merlangus (unless otherwise stated) c...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ogut H,Palm HW

    更新日期:2005-06-01 00:00:00

  • Antileishmanial efficacy of fluconazole and miltefosine in combination with an immunomodulator--picroliv.

    abstract::The chemotherapy of visceral leishmaniasis (VL) has several limitations including resistance and toxicity of the existing drugs. Downregulation of immune system further aggravates the problems. To combat this situation, leishmanicidal efficacy of already marketed standard antifungal drug, fluconazole under the approac...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Shakya N,Sane SA,Gupta S

    更新日期:2011-04-01 00:00:00

  • Echinostoma trivolvis: mating behavior of adults raised in hamsters.

    abstract::Echinostoma trivolvis adults grown in golden hamsters were treated in vitro with [3H]-tyrosine to label sperm and transplanted to uninfected hamsters alone or with four to six unlabeled adults. After 4-5 days, worms were recovered, processed for autoradiography, and observed for silver grains over their seminal recept...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Nollen PM

    更新日期:1993-01-01 00:00:00

  • A simple method for long-term storage of Acanthamoeba species.

    abstract::We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyp...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Axelsson-Olsson D,Olofsson J,Ellström P,Waldenström J,Olsen B

    更新日期:2009-03-01 00:00:00

  • Historic importance of some aspects of research by O. Wilford Olsen on hookworms (Uncinaria lucasi) in northern fur seals (Callorhinus ursinus) and Steller sea lions (Eumatopias jubatus) in 1951 on St. Paul Island, Alaska.

    abstract::The purpose of this paper is to disseminate, more widely, certain historically important aspects of research by O. Wilford Olsen on hookworms (Uncinaria lucasi) in northern fur seals (Callorhinus ursinus) and Steller sea lions (Eumatopias jubatus) in 1951 on St. Paul Island, Alaska. Highly meaningful facets of the res...

    journal_title:Parasitology research

    pub_type: 历史文章,杂志文章


    authors: Lyons ET

    更新日期:2005-03-01 00:00:00

  • Insecticidal properties of essential oils from Lippia turbinata and Lippia polystachya (Verbenaceae) against Culex quinquefasciatus (Diptera: Culicidae).

    abstract::Mosquitoes are important vectors of diseases to humans and domestic animals. Chemical control of vectors remains a main resource for the prevention and control of vector-borne diseases. Due to the development of insecticide resistance and risks to human health and the environment of synthetic compounds, the search for...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Gleiser RM,Zygadlo JA

    更新日期:2007-10-01 00:00:00

  • Blood parasites of bird communities in Sri Lanka and their mosquito vectors.

    abstract::Avian blood parasites have been shown to have significant health effects on avifauna worldwide. Sri Lanka, a tropical island rich with resident and migratory birds, has not been properly evaluated for avian blood parasites or their vectors. We investigated the presence of avian haemoparasites in Sri Lankan birds and t...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Chathuranga WGD,Fernando BR,Weereratne TC,Karunaratne SHPP,De Silva WAPP

    更新日期:2021-01-16 00:00:00

  • Taxonomy on three novel species of Sphaeromyxa Thélohan 1892 (Myxozoa, Bivalvulida, Sphaeromyxidae) with insight into the evolution of the genus.

    abstract::Three new myxosporeans of the genus Sphaeromyxa Thélohan 1892 were discovered from the coastal waters off Xiamen in the East China Sea and characterized based on morphological and SSU rDNA data. Sphaeromyxa photopectoralis sp. n. was described from Photopectoralis bindus, and Sphaeromyxa sebastisca sp. n. was describe...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Chen W,Yang C,Whipps CM,Peng Z,Zhao Y

    更新日期:2020-05-01 00:00:00

  • Cellular reactions in the small intestine of rats after infection with Fasciola hepatica.

    abstract::Groups of five rats each were infected with metacercariae of Fasciola hepatica according to two experimental procedures. In the first experiment, they received 20 metacercariae 0, 1, 2 or 3 times at intervals of 4 weeks. In the second, both the inoculation dose and the interval between doses were varied. After the ani...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Charbon JL,Spähni M,Wicki P,Pfister K

    更新日期:1991-01-01 00:00:00

  • Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum.

    abstract::Giardia lamblia and Cryptosporidium parvum are recognized as the most common protozoan infections in Saudi Arabia. Microscopic examination of stool samples, either direct or concentrated, for the recovery of G. lamblia cysts and trophozoites and C. parvum oocysts is still the most commonly used for the diagnosis of bo...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Elsafi SH,Al-Maqati TN,Hussein MI,Adam AA,Hassan MM,Al Zahrani EM

    更新日期:2013-04-01 00:00:00

  • Nitric oxide involvement in experimental Trypanosoma cruzi infection in Calomys callosus and Swiss mice.

    abstract::Nitric oxide (NO) production by peritoneal macrophages was evaluated in Calomys callosus and Swiss mice during the course of infection with two strains of Trypanosoma cruzi. In C. callosus, no NO production was detected throughout the period of observation in animals infected with either parasite strain, except for a ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: de Oliveira L,Borges MM,Leal RC,Assreuy J,Kloetzel JK

    更新日期:1997-01-01 00:00:00

  • Cimex pipistrelli (Heteroptera, Cimicidae) and the dispersal propensity of bats: an experimental study.

    abstract::Previous results have shown frequent movements of crevice-dwelling bats between different shelters. Low roost fidelity of some dwelling bat species reduces the reproductive success of ectoparasites. The question of whether high densities of bat bugs (Cimex pipistrelli) represent a cost for crevice-dwelling bats (Pipis...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bartonicka T

    更新日期:2008-12-01 00:00:00

  • Larvicidal efficacy of Adhatoda vasica (L.) Nees against the bancroftian filariasis vector Culex quinquefasciatus Say and dengue vector Aedes aegypti L. in in vitro condition.

    abstract::The larvicidal activities of methanolic fractions from Adhatoda vasica leaf extracts were investigated against the bancroftian filariasis vector Culex quinquefasciatus and dengue vector Aedes aegypti. The results indicated that the mortality rates was high at 100, 150, 200 and 250 ppm of methanol extract of fractions ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Thanigaivel A,Chandrasekaran R,Revathi K,Nisha S,Sathish-Narayanan S,Kirubakaran SA,Senthil-Nathan S

    更新日期:2012-05-01 00:00:00

  • Early intrauterine embryonic development in Khawia sinensis Hsü, 1935 (Cestoda, Caryophyllidea, Lytocestidae), an invasive tapeworm of carp (Cyprinus carpio): an ultrastructural study.

    abstract::Intrauterine embryonic development in the caryophyllidean tapeworm Khawia sinensis has been investigated using transmission electron microscopy and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate for glycogen. Contrary to previous light microscopy findings that reported the release of non-...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bruňanská M,Mackiewicz JS,Młocicki D,Swiderski Z,Nebesářová J

    更新日期:2012-02-01 00:00:00

  • Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening.

    abstract::Toxoplasma gondii is an opportunistic protozoan parasite that differentiates into latent cysts (bradyzoite) that can be reactivated during immunosuppression. TgSRCAP (Toxoplasma gondii Snf2-related CBP activator protein) is a SWI2/SNF2 family chromatin remodeler whose expression increases during cyst development. Iden...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Nallani KC,Sullivan WJ Jr

    更新日期:2005-03-01 00:00:00

  • Prevalence and seasonal variation of gastrointestinal nematodes and coccidia infecting ovine grazing on communal rangelands in the Eastern Cape, South Africa.

    abstract::Nematodes and coccidia are major pathogens causing parasitic infections in livestock production. This study aimed to determine the prevalence and seasonality of gastrointestinal helminths and coccidia in communal sheep grazing in humid, semi-humid and arid zones of the Eastern Cape Province. A total of 3299 faecal sam...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Jansen MS,Nyangiwe N,Yawa M,Dastile M,Mabhece V,Muchenje V,Mpendulo TC

    更新日期:2020-12-01 00:00:00