Abstract：
:Telomerase, consisting of telomerase RNA and telomerase reverse transcriptase (TERT), is responsible for the maintenance of the end of linear chromosomes. TERT, as the catalytic subunit of telomerase, plays a critical role in telomerase activity. Researches indicate TERT-associated proteins participate in the regulation of telomerase assembly, posttranslational modification, localization, and enzymatic function. Here, the telomerase RNA-binding domain of Eimeria tenella TERT (EtTRBD) was cloned into pGBKT7 and performed as the bait. α-Galactosidase assay showed that the bait plasmid did not activate Gal4 reporter gene. Further, we isolated an EtTRBD-associated protein, 14-3-3, by yeast two-hybrid screening using the constructed bait plasmid. To confirm the interaction, EtTRBD and 14-3-3 were expressed by prokaryotic and eukaryotic expression systems. Pull-down assays by purified proteins demonstrated a direct bind between EtTRBD and 14-3-3. Co-immunoprecipitation techniques successfully validated that 14-3-3 interacted with EtTRBD in 293T cells. The protein-protein interaction provides a starting point for more in-depth studies on telomerase and telomere regulation in E. tenella.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Zhao N,Gong P,Cheng B,Li J,Yang Z,Li H,Yang J,Zhang G,Zhang Xdoi
10.1007/s00436-014-4108-1subject
Has Abstractpub_date
2014-10-01 00:00:00pages
3885-9issue
10eissn
0932-0113issn
1432-1955journal_volume
113pub_type
杂志文章abstract：:An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h. ...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2009-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-08-01 00:00:00
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doi：10.1007/s00436-008-1111-4
更新日期：2008-10-01 00:00:00
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
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pub_type： 临床试验,杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
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journal_title：Parasitology research
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更新日期：2011-09-01 00:00:00
