Abstract：
:This study aimed to explore Toxoplasma gondii nucleus coding apicoplast protein acyl carrier protein (ACP) synthesis and trafficking in delayed death. The recombinant T. gondii ACP was expressed by prokaryotic expression method, and anti-ACP polyclonal antibody was obtained from rabbit immune. T. gondii "delayed death" was induced by clindamycin (CLDM), and ACP transcription was determined by real-time PCR assay. The expression of ACP with transit type (t-ACP) and mature type (m-ACP) was determined by Western blotting with anti-ACP polyclonal antibody. The mutant-expressed ACP fused with green fluorescent protein (GFP) tag was constructed by pHX-ACP-GFP. The distribution of ACP in "delayed death" was observed by ACP-GFP fusion protein with a confocal microscope. T. gondii ACP transcription and t-ACP expression had no significant decrease in the early 4 h of "delayed death," but there has been a significant decrease in 6 h. The expression of m-ACP had a significant decrease in 4 h which occurred earlier than the t-ACP expression. The number of brightly dot green fluorescence in ACP-GFP mutant decreased with prolonged time. There was very little brightly dot green fluorescence in ACP-GFP mutant when treated with CLDM for 6 h. CLDM could suppress apicoplast proliferation and induce T. gondii "delayed death"; however, it could not directly suppress nucleus coding ACP transcription and expression. T. gondii lacking of apicoplast had a barrier of transit peptide cleavage and t-ACP could not be transformed into m-ACP. The reason for the decrease in ACP expression could be due to excessive t-ACP synthesis in tachyzoites resulting in a negative feedback for the ACP coding gene transcription.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Wu L,Shen J,Zhou Y,Wang X,Wu L,Jiang X,Chen Sdoi
10.1007/s00436-014-4281-2subject
Has Abstractpub_date
2015-03-01 00:00:00pages
1099-105issue
3eissn
0932-0113issn
1432-1955journal_volume
114pub_type
杂志文章abstract：:The parasite community structure of the neotropical cormorant, Phalacrocorax brasilianus, from two lagoons (Coyuca and Tres Palos) from Guerrero state, México, was examined. Fourteen species of adult helminths (6,391 individuals) from 48 cormorants were identified: 9 digeneans, 1 acanthocephalan, 1 cestode, and 3 nema...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2377-5
更新日期：2011-11-01 00:00:00
abstract：:Seventy specimens of Hypsiboas albopunctatus (Anura: Hylidae) were collected and surveyed for endoparasites in Brasília, Brazil at the end of the rainy season (February and March) in 2005. Sixteen males (22.86%) were infected by Rhabdias sp., Aplectana sp., Cosmocerca sp., and Falcaustra mascula. Rhabdias sp., a lung ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0797-z
更新日期：2008-03-01 00:00:00
abstract：:The recombinant surface protein of Theileria annulata (TaSP) was used in the standardization and validation of an enzyme linked immunosorbent assay (ELISA) for the detection of circulating antibodies against tropical theileriosis. ELISA data were expressed as the percentage positivity (PP) of the reactivity of an inte...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1055-7
更新日期：2004-03-01 00:00:00
abstract：:Spermiogenesis and the ultrastructure of the spermatozoon of the bothriocephalidean cestode Clestobothrium crassiceps (Rudolphi, 1819), a parasite of the teleost fish Merluccius merluccius (Linnaeus, 1758), have been studied by means of transmission electron microscopy. Spermiogenesis involves firstly the formation of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2446-9
更新日期：2012-01-01 00:00:00
abstract：:The principal health problem in small ruminants is helminthiasis and the rapid development of nematode resistance to anthelminthics has limited the success of control in several countries, stimulating the search for alternatives. In this study, extracts of immature fruits of the mango Mangifera indica L. var Ubá were ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3017-4
更新日期：2012-12-01 00:00:00
abstract：:Bimiracidial infections of Lymnaea ovata with Fasciola hepatica were performed under laboratory conditions to determine the susceptibility of snails from six French populations to trematode infection. In five populations of L. ovata the prevalence of infection in the 1-mm groups ranged between 2.7% and 43.7% at day 35...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050013
更新日期：2000-01-01 00:00:00
abstract：:In the present study, Diplectanum laubieri was redescribed from material obtained from wild and cultured fish. The population dynamics of D. aequans and D. laubieri under culture conditions was studied. The prevalence and mean intensity (average number of worms per parasitized fish) were 80.64% and 112 for D. aequans ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930906
更新日期：1991-01-01 00:00:00
abstract：:Tetratrichomonas didelphidis is a flagellate protozoan found in the intestine of the opossum. The parasite lives in a hostile and stressed environment, where it interacts with microorganisms and can survive under extreme conditions for growth, involving strict anaerobiosis or equilibration with air and abundance or ab...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1015-2
更新日期：2004-01-01 00:00:00
abstract：:The present study reports on the antifilarial activity of a marine sponge Haliclona exigua (phylum Porifera). The crude methanol extract and n-butanol-soluble fraction killed adult Brugia malayi at 31.25-microg/ml concentration (both in motility and 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1555-1
更新日期：2009-10-01 00:00:00
abstract：:In an experiment including 8 groups of 15 mice, the effect of migrating Ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to Pasteurella multocida was investigated. Following aerosol exposure to P. multocida, mice with migrating A. suum in their lungs developed more severe pne...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931575
更新日期：1992-01-01 00:00:00
abstract：:Feline lungworms infect the respiratory tract of wild and domestic cats, causing infection often associated with clinical disease. Until recently, Aelurostrongylus abstrusus has been considered the most relevant species of lungworm, while Troglostrongylus brevior was considered of less significance. Fecal samples of f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06852-8
更新日期：2020-10-01 00:00:00
abstract：:The insect growth regulator, Lufenuron, at concentrations of even multiples of LC(90) (determined under laboratory conditions) was tested against the III instar larvae of Aedes aegypti under simulated field conditions. For all concentrations tested, 100 % mortality of the larvae was observed within 24 h of exposure to...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2968-9
更新日期：2012-09-01 00:00:00
abstract：:Specific anti-newborn larva antibodies present in the serum of rats chronically infected with Trichinella spiralis were incapable of inducing killing of newborn larvae by activation of normal peritoneal cells. These late antibodies blocked the cytotoxic reaction induced by early antibodies produced a few weeks after i...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050072
更新日期：1996-01-01 00:00:00
abstract：:The distribution of actin filaments in the spermatogenic cells of Fasciola hepatica was determined using a fluorescent derivative of phalloidin. Actin was localised primarily in the region of separation of a secondary spermatogonium from a primary spermatogonium, in the inner faces at the centre of four-cell clusters ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00935425
更新日期：1991-01-01 00:00:00
abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Echinococcus coproantigens in fecal samples from dogs, dingoes or foxes infected with either E. granulosus or E. multilocularis. The ELISA was based on protein-A-purified polyclonal antibodies [anti-E. granulosus excretory/secretory (E/S) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00937088
更新日期：1992-01-01 00:00:00
abstract：:The artemisinin-based combination therapy (ACT) is adopted by several countries as first line for malaria treatment in the last decade. Concomitantly, the World Health Organization and other research reports showed a dramatic decline in malaria burden in terms of morbidity, mortality and treatment failure (TF). The op...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-009-1700-x
更新日期：2010-02-01 00:00:00
abstract：:Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2898-6
更新日期：2012-09-01 00:00:00
abstract：:The seasonal dynamics of Gyrodactylus rhodei, a monogenean ectoparasite of bitterling (Rhodeus sericeus), was studied from June 2000 to May 2001 in the Kyjovka River, Czech Republic. A negative relationship between prevalence and intensity of infection of G. rhodei and water temperature was found. Metrical variability...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1311-0
更新日期：2005-04-01 00:00:00
abstract：:Mosquito bites can elicit dermal hypersensitivity reactions, but little is known about the chemotactic factors for host leukocytes in mosquito saliva. In this study, we determined that saliva from a malarial vector mosquito, Anopheles stephensi, possesses intense neutrophil chemotactic activity. In contrast, the midgu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000355
更新日期：2001-05-01 00:00:00
abstract：:We studied in vivo immune response to fleas in two gerbils, Gerbillus dasyurus and Gerbillus andersoni allenbyi, which differed in their natural species richness of flea assemblages and prevalence of infestation. G.dasyurus is parasitized naturally by several flea species, but the prevalence of infestation is low, whe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1215-4
更新日期：2004-10-01 00:00:00
abstract：:Spirometra erinacei is a pseudophyllidean tapeworm which inhabits the intestines of cats and dogs. The infections are usually asymptomatic in these animals, but the infection of the plerocercoid larvae of the parasite, spargana, cause sparganosis in other vertebrates, including human. In this study, we identified a ge...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1714-4
更新日期：2010-02-01 00:00:00
abstract：:Praziquantel, due to high efficacy, excellent tolerability, few and transient side effects, simple administration, and competitive cost, is virtually the only drug of choice for treatment of human schistosomiasis. Treatment of schistosomiasis has shown great advances with the introduction of the drug into the therapeu...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-011-2670-3
更新日期：2011-12-01 00:00:00
abstract：:The hypersaline lagoons located in evaporation basins or salars (salt flats) in the Atacama Desert are extreme environments harbouring a specialised biota able to survive harsh conditions for life. The knowledge of the parasitic biodiversity of these extreme habitats is still scarce despite their functional importance...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06768-3
更新日期：2020-10-01 00:00:00
abstract：:Changes in the hepatocytes of male hamsters (Mesocricetus auratus) and jirds (Meriones unguiculatus) at 220 days after experimental infection with the liver fluke Opisthorchis viverrini were studied by light and electron microscopy. The hepatocytes of the control group were characterized by an intracellular compartmen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931823
更新日期：1993-01-01 00:00:00
abstract：:Cystic echinococcosis (CE) is a widespread zoonosis. For treating single echinococcal cysts during the last decades, therapeutic puncture of the cyst, aspiration, injection of a scolicide, and re-aspiration (PAIR) has been established as a minimal-invasive alternative method to surgery. A recent review on the complica...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4275-0
更新日期：2015-02-01 00:00:00
abstract：:We have previously reported the involvement of cyclic AMP in regulating flagellar waveforms in Leishmania. Here, we investigated the roles of calcium, calmodulin, and calcineurin in flagellar motility regulation in L. donovani. Using high-speed videomicroscopy, we show that calcium-independent calmodulin and calcineur...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5608-6
更新日期：2017-11-01 00:00:00
abstract：:Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Satureja cuneifolia and Melissa officinalis. In th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2744-2
更新日期：2012-06-01 00:00:00
abstract：:The G-Protein-coupled receptor Hc110-R of Haemonchus contortus and its orthologue in Caenorhabditis elegans, the latrophilin-like protein 1 (LAT-1), were shown to play a role in the mode of action of the new anthelmintic compound emodepside. C. elegans LAT-1 knockout mutants showed a decreased paralysing effect of emo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1500-3
更新日期：2009-08-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-beta) is a highly conserved cytokine that has a well-known regulatory role in immunity, but also in organ development of most animal species including helminths. Homologous tgf-b genes and mRNA have been detected in the filaria Brugia malayi. The in situ protein expression is unkno...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1450-9
更新日期：2009-09-01 00:00:00
abstract：:Plasmodium falciparum (Pf) refurbishes the infected erythrocytes by exporting a myriad of parasite proteins to the host cell. A novel exported protein family 'Plasmodium Helical Interspersed Subtelomeric' (PHIST) has gained attention for its significant roles in parasite biology. Here, we have collected and analysed a...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-019-06420-9
更新日期：2019-10-01 00:00:00
