Purification and cellular localization of the Entamoeba histolytica transcarboxylase.

Abstract:

:Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Barbosa-Cabrera E,Salas-Casas A,Rojas-Hernández S,Jarillo-Luna A,Abarca-Rojano E,Rodríguez MA,Campos-Rodríguez R

doi

10.1007/s00436-012-2898-6

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

1401-5

issue

3

eissn

0932-0113

issn

1432-1955

journal_volume

111

pub_type

杂志文章