Abstract：
:We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blastocystis by an ova and parasite test as well as a conventional PCR assay were positive for Blastocystis using our real-time LC PCR assay. Diagnosis of Blastocystis infections using this sensitive method, including DNA extraction and real-time PCR, only requires 3 h. The lower limit of detection for Blastocystis in stool using the real-time LC PCR assay was calculated to be 760 cells of Blastocystis per 100 mg of stool, an estimated 760 parasites per reaction. The assay did not cross-react with Ruminococcus hansenii, Anarococcus hydrogenalis, Bifidobacterium adolescentis, Fusobacterium prausnitzii, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, or Lactobacillus acidophilus. Because of the ease of use, sensitivity, specificity, and increase in Blastocystis infections in the USA we believe this assay has the potential to be useful as a clinical diagnosis tool of Blastocystis infection.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Jones MS 2nd,Ganac RD,Hiser G,Hudson NR,Le A,Whipps CMdoi
10.1007/s00436-008-1006-4subject
Has Abstractpub_date
2008-08-01 00:00:00pages
551-7issue
3eissn
0932-0113issn
1432-1955journal_volume
103pub_type
杂志文章abstract：:Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050568
更新日期：1999-05-01 00:00:00
abstract：:Cysticercoids as well as 6-, 10-, and 14-day Hymenolepis diminuta were evaluated in terms of enzymatic activities related to phosphoenolpyruvate (PEP) utilization and mitochondrial succinate accumulation. The data obtained support a transition toward anaerobic electron-transport-dependent succinate accumulation, chara...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050487
更新日期：1998-10-01 00:00:00
abstract：:Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4327-0
更新日期：2015-04-01 00:00:00
abstract：:The localisation and distribution of the cytoskeletal protein tubulin in the adult liver fluke Fasciola hepatica have been determined by an indirect immunofluorescence technique using a monoclonal antibody raised against beta-tubulin. Tubulin was demonstrated in the tegumental syncytium and in the tegumental cell bodi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931649
更新日期：1992-01-01 00:00:00
abstract：:Analyses were made on the adenosine transporter-1 gene in Trypanosoma brucei (TbAT1), encoding a P2-like nucleoside transporter, from T. brucei brucei field stocks to investigate a possible link between the presence of mutations in this gene and isometamidium resistance. We have analysed the gene from 11 isometamidium...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0141-z
更新日期：2006-08-01 00:00:00
abstract：:The fine structure of the infective hexacanths of Echinococcus multilocularis was examined with particular emphasis on the functional ultrastructure of penetration glands and nerve cells directly involved in the mechanism of initial host infection. The oncosphere contains two types of penetration glands, PG1 and PG2, ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5957-9
更新日期：2018-08-01 00:00:00
abstract：:The effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. This is due to the fact that some invasive mosquitoes, with special reference to the Aedes genus, are particularly difficult to control, due to their high ecological plasticity. Moreover, the indiscriminate over...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5395-0
更新日期：2017-04-01 00:00:00
abstract：:Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagn...
journal_title：Parasitology research
pub_type： 杂志文章,meta分析
doi：10.1007/s00436-015-4579-8
更新日期：2015-09-01 00:00:00
abstract：:The history of European bison Bison bonasus Linnaeus, 1758 has been stormy since its extinction in the wild after the First World War. Due to the fact that the species was restored from just 12 founders, further expansion has suffered from low genetic variability, rendering the bison vulnerable to various pathogens du...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5663-z
更新日期：2018-01-01 00:00:00
abstract：:Leishmaniasis is a geographically widespread disease caused by protozoan parasites belonging to the genus Leishmania and transmitted by certain species of sand fly. This disease still remains endemic in China, especially in the west and northwest frontier regions. A recent ITS1 phylogeny of Chinese Leishmania isolates...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2239-6
更新日期：2011-07-01 00:00:00
abstract：:The purpose of the present study was to investigate the acaricidal and larvicidal activity against the larvae of Haemaphysalis bispinosa Neumann (Acarina: Ixodidae) and larvae of hematophagous fly Hippobosca maculata Leach (Diptera: Hippoboscidae) and against the fourth-instar larvae of malaria vector, Anopheles steph...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2473-6
更新日期：2012-08-01 00:00:00
abstract：:This study was made to investigate efficacy of eprinomectin against to Toxocara canis in dogs. In the study, 20 stray dogs naturally infected with T. canis were divided into two groups as treatment (ten dogs) and control (ten dogs). Eprinomectin (100 microg/kg, Eprinex 250 ml) was given to treatment group dogs orally,...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0776-4
更新日期：2008-02-01 00:00:00
abstract：:We have previously reported the involvement of cyclic AMP in regulating flagellar waveforms in Leishmania. Here, we investigated the roles of calcium, calmodulin, and calcineurin in flagellar motility regulation in L. donovani. Using high-speed videomicroscopy, we show that calcium-independent calmodulin and calcineur...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5608-6
更新日期：2017-11-01 00:00:00
abstract：:Three new myxosporeans of the genus Sphaeromyxa Thélohan 1892 were discovered from the coastal waters off Xiamen in the East China Sea and characterized based on morphological and SSU rDNA data. Sphaeromyxa photopectoralis sp. n. was described from Photopectoralis bindus, and Sphaeromyxa sebastisca sp. n. was describe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06656-w
更新日期：2020-05-01 00:00:00
abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Echinococcus coproantigens in fecal samples from dogs, dingoes or foxes infected with either E. granulosus or E. multilocularis. The ELISA was based on protein-A-purified polyclonal antibodies [anti-E. granulosus excretory/secretory (E/S) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00937088
更新日期：1992-01-01 00:00:00
abstract：:Drashia megastoma Rudolphi, 1819, Habronema muscae Carter, 1861 and Habronema microstoma Schneider, 1866 are found in the stomach of equine definitive hosts and are known to cause pathogenic effects in the stomach wall, skin, eye and occasionally other sites. These nematodes utilise either house flies or stable flies ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0637-1
更新日期：2007-10-01 00:00:00
abstract：:Ivermectin at single doses of 0.2-1.0 mg/kg body weight reduced the microfilarial counts of subperiodic Brugia malayi in Presbytis cristata by 59.9%-89.6% of initial counts, 4 weeks after treatment. Adult filaricidal activity was poor, live adult worms being recovered from all animals at autopsy. There was no serious ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00539462
更新日期：1988-01-01 00:00:00
abstract：:This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of P...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2514-1
更新日期：2012-02-01 00:00:00
abstract：:Antigens derived from Echinococcus granulosus, Taenia hydatigena and T. pisiformis cyst fluids, T. solium cysticerci, E. multilocularis protoscoleces and E. vogeli cyst membranes were examined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) using four monoclonal antibodies (mAb) to E. gran...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931225
更新日期：1993-01-01 00:00:00
abstract：:Human hydatidosis is characterized by a prolonged coexistence of Echinococcus granulosus and its host without effective rejection of the parasite. This parasitic infection constitutes a major health problem in Algeria. In this study, we investigated in vivo production of nitrite (NO(2)(-) + NO(3)(-)) in sera of Algeri...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3181-6
更新日期：2013-02-01 00:00:00
abstract：:Ultrastructure of spermiogenesis and mature spermatozoon of Cotylophoron cotylophorum are described with transmission electron microscopy. The description gives evidence of some characteristics of this digenean. The intercentriolar body situated between two striated roots and two centrioles exhibits a symmetric plane....
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-0944-1
更新日期：2008-06-01 00:00:00
abstract：:The morphology and ecology are described for four sibling species of digenean metacercariae: Meiogymnophallus minutus (Cobbold 1859), M. strigatus (Lebour 1908) from the estuarine cockle Cerastoderma edule L. and M. fossarum (Bartoli, 1965) and M. rebecqui (Bartoli, 1983) comb. nov. from the brackish water lagoon cock...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050153
更新日期：1996-01-01 00:00:00
abstract：:A survey of the myxosporean fauna of Australian marine fishes revealed the presence of three previously unreported species of Unicapsula (Multivalvulida: Trilosporidae) from sites off Southeast Queensland, off Lizard Island on the Great Barrier Reef, Queensland, and from Jurien Bay in Western Australia. Morphometric d...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3467-3
更新日期：2013-08-01 00:00:00
abstract：:The objective of the study was to estimate the prevalence of helminths in the horse population of the state of Brandenburg, Germany. One hundred and twenty-six horse farms in the state were selected by randomised stratified sampling. In total, 1,407 horses across all farms were examined coproscopically. The experiment...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2362-z
更新日期：2011-05-01 00:00:00
abstract：:As part of a biological inventory of the Rio Lacantún basin in the Biosphere Reserve of Montes Azules from Chiapas State (southeastern, Mexico), the following monogenoid ectoparasites infecting the external surface of the anal opening and the gill lamellae of the freshwater fish Astyanax aeneus (Characidae) in ten str...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3126-0
更新日期：2013-01-01 00:00:00
abstract：:NADH-ubiquinone oxidoreductase activity is present in mitochondrial lysates of Phytomonas serpens. Rotenone at 2-10 microM inhibited the activity 50-75%, indicating that it belongs to respiratory complex I. The activity was also inhibited 50-60% in the presence of 10-30 nM atovaquone suggesting that inhibition of comp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1058-4
更新日期：2004-03-01 00:00:00
abstract：:In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5049-7
更新日期：2016-08-01 00:00:00
abstract：:Vector-borne diseases caused by mosquitoes are one of the major economic and health problems in many countries. The Aedes aegypti mosquito is a vector of several diseases in humans like yellow fever and dengue. Vector control methods involving the use of chemical insecticides are becoming less effective due to develop...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3811-2
更新日期：2014-05-01 00:00:00
abstract：:Mosquito adults usually need to ingest sugar from nectar host plants to sustain their metabolic needs. Mosquitoes could be differentially attracted by various flowering plant species, and the volatiles were thought to be important factors attributed to the differential attractiveness. However, whether mosquitoes' pref...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5664-y
更新日期：2018-01-01 00:00:00
abstract：:Strongyloides stercoralis can cause severe infection both in humans and dogs. Coproparasitological examination has low sensitivity for the diagnosis of this parasite; hence, different diagnostic techniques have been implemented. However, serology and molecular methods have been assessed almost exclusively in humans. I...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5468-0
更新日期：2017-07-01 00:00:00
