Fasciola hepatica: localization and partial characterization of tubulin.

Abstract:

:The localisation and distribution of the cytoskeletal protein tubulin in the adult liver fluke Fasciola hepatica have been determined by an indirect immunofluorescence technique using a monoclonal antibody raised against beta-tubulin. Tubulin was demonstrated in the tegumental syncytium and in the tegumental cell bodies and their cytoplasmic connections with the surface syncytium. Immunostaining was also evident in the nerve fibres innervating sensory receptors in the tegument, in the nerve plexus innervating the sub-tegumental musculature and in the cytoplasmic extensions of the nurse cells within the vitelline follicle. Immunoblotting of whole fluke fractions produced a single band corresponding to a molecule of approximately 54 kDa in size. This figure corresponds with previous data obtained on tubulin from other helminth and eukaryotic sources.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Stitt AW,Fairweather I,Trudgett AG,Johnston CF

doi

10.1007/BF00931649

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

103-7

issue

2

eissn

0932-0113

issn

1432-1955

journal_volume

78

pub_type

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