Abstract：
:Thus far, Entamoeba species have been classified based on morphology such as the number of nuclei in mature cysts and their hosts. Using recently developed molecular tools, ruminant Entamoeba spp. are currently classified into four species/genotypes: E. bovis and Entamoeba ribosomal lineages (RL) 1, 2, and 4. However, the distribution or pathogenicity of ruminant Entamoeba has not been well documented. In the present study, we examined a total of 25 fecal and seven environmental samples collected from six farms in Japan from 2016 to 2017 by the floatation method and PCR and sequencing analyses. Consequently, we detected Entamoeba cysts in 18 of 25 cattle samples and four of the seven environmental samples, including soil and drinking water, by microscopic examinations. In sequential examinations, Entamoeba-positive cattle were found to shed cysts without any clinical symptoms for more than 8 months. By PCR for molecular identification, isolates in ten cattle and one soil sample were successfully sequenced and formed a cluster of E. bovis, which was separated from those of other Entamoeba species/genotypes such as RL1-4 in phylogenetic analysis. To our knowledge, this is the first report about E. bovis in Japan, and our results may implicate that E. bovis is not pathogenic.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Matsubayashi M,Matsuura Y,Nukata S,Daizi Y,Shibahara T,Teramoto I,Matsuo T,Uni S,Hatta T,Kaneko A,Tsuji N,Sasai Kdoi
10.1007/s00436-017-5689-2subject
Has Abstractpub_date
2018-01-01 00:00:00pages
339-342issue
1eissn
0932-0113issn
1432-1955pii
10.1007/s00436-017-5689-2journal_volume
117pub_type
杂志文章abstract：:Haemoproteus spp. and Plasmodium spp. are blood parasites that occur in birds worldwide. Identifying the species within this group is complex, especially in wild birds that present low parasitemia when captured, making morphological identification very difficult. Thus, the use of alternative tools to identify species ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06908-9
更新日期：2020-12-01 00:00:00
abstract：:Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5102-6
更新日期：2016-08-01 00:00:00
abstract：:The relationships between inflammation in organs with Toxocara canis larval migration and matrix metalloproteinase-9 (MMP-9) were investigated following the infection of mice with 1,000 infective eggs. Gelatinase activity was defined by gelatin zymography, optimum pH, inhibitor specificity and Western blot analysis. M...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1271-9
更新日期：2005-02-01 00:00:00
abstract：:Pharmacokinetics and anthelmintic activity of topical eprinomectin in goats prevented from physical contact to others and self-grooming were studied. Sixteen approximately 7 months old male castrated German White Noble goats harbouring induced infections of gastrointestinal nematode parasites were included in the stud...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4072-9
更新日期：2014-11-01 00:00:00
abstract：:Avian malaria is of significant ecological importance and serves as a model system to study broad patterns of host switching and host specificity. The erythrocyte invasion mechanism of the malaria parasite Plasmodium is mediated, in large part, by proteins of the erythrocyte-binding-like (ebl) family of genes. However...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3211-4
更新日期：2013-03-01 00:00:00
abstract：:The taxonomy and classification of the family Opisthorchiidae have been revised by several authors with the exclusion or synonymization of some genera. The genus Hepatiarius Feizullaev, 1961 accommodated two species: Hepatiarius sudarikovi Feizullaev, 1961 and H. longissimus Linstow, 1883. Recently, some experts have ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06227-8
更新日期：2019-03-01 00:00:00
abstract：:A pellet freezing method, one type of rapid freezing protocol, was established for Trichomonas vaginalis. As cryoprotectants, 5-20% dimethylsulfoxide (DMSO), ethylene glycol, propylene glycol, and glycerol were used. Of these, DMSO showed a high protective effect, with a maximum effect at a concentration of 20%. No ef...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0559-y
更新日期：2007-09-01 00:00:00
abstract：:A total of 37 juvenile Etmopterus spinax from the Norwegian Deep were examined for stomach contents and metazoan ecto- and endoparasites. These squaliform elasmobranchs were caught by benthopelagic net in May 2001 at a depth of 194-214 m. The euphausiid Meganyctiphanes norvegica and the teleost Maurolicus muelleri wer...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0741-1
更新日期：2003-03-01 00:00:00
abstract：:Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3262-6
更新日期：2013-05-01 00:00:00
abstract：:The present paper reports the ability of egg-propagated gametocyte vaccine to induce protective immunity against Eimeria tenella infections in offspring chicks. For this purpose, breeding hens were immunized with adjuvanted gametocyte vaccine orally, being the best vaccine in our previous studies. Eggs collected 2 wee...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0382-x
更新日期：2007-04-01 00:00:00
abstract：:This study was conducted to observe the localization and to compare methods for isolation of minute Ascaridia galli larvae in chicken intestine. Firstly, six 7-week-old layer pullets were orally infected with 2,000 embryonated A. galli eggs and necropsied either at 3, 5 or 7 days post infection (dpi). More than 95 % o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3079-3
更新日期：2012-12-01 00:00:00
abstract：:Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagn...
journal_title：Parasitology research
pub_type： 杂志文章,meta分析
doi：10.1007/s00436-015-4579-8
更新日期：2015-09-01 00:00:00
abstract：:Free-living amoebae (FLA) are opportunistic and ubiquitous protozoa that are widely found in various environmental sources. They are known to cause serious human infections. The aim of our study was to detect FLA and Acanthamoeba spp. in hospital water circuits. Eighty-four water samples were collected over a period o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4788-1
更新日期：2016-01-01 00:00:00
abstract：:To elucidate whether Kudoa septempunctata was able to live in the human intestine, we assessed viability of K. septempunctata sporoplasms under conditions that mimicked human and ragworm digestive tracts. To study the effect of osmotic pressure on viability, sporoplasms were incubated in 0.9 or 3.4 % sodium chloride s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5036-z
更新日期：2016-06-01 00:00:00
abstract：:Tungiasis is endemic in many countries in Latin America, the Caribbean and sub-Saharan Africa, and it is associated with severe morbidity. The pathophysiological and immunological characteristics of the ectoparasitosis are not well understood, and no effective therapy is currently available. The aim of this study was ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0731-4
更新日期：2007-12-01 00:00:00
abstract：:Leishmania spp. and Trypanosoma cruzi are parasites belonging to the Trypanosomatidae family and the causative agents for two very important neglected tropical diseases (NTDs), namely leishmaniasis and trypanosomiasis, respectively. Together, they affect millions of people worldwide and the number of cases is constant...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-020-07035-1
更新日期：2021-01-06 00:00:00
abstract：:A novel approach to control strategies for integrated blood-feeding parasite management is in high demand, including the use of biological control agents. The present study aims to determine the efficacy of optimized crude extract of actinomycetes strain LK1 as biological control agent against the fourth-instar larvae...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3585-y
更新日期：2013-11-01 00:00:00
abstract：:In the present study, the morphology and morphometric characterization of Dujardinnascaris mujibii (Heterocheilidae) and Hysterothylacium aduncum (Anisakidae), new nematode parasites infecting the sea bream Pagrus pagrus (Osteichthyes, Sparidae), were described for the first time from the Gulf of Suez and Hurghada Cit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3270-6
更新日期：2013-02-01 00:00:00
abstract：:We report on serine protease activity in cercarial secretions (CSs) from the bird parasite Trichobilharzia ocellata. Using a colorigenic substrate, the biochemical properties of this enzyme were studied and its activity was compared to the homologous one in CSs from the human parasite Schistosoma mansoni. The specific...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0597-4
更新日期：2002-06-01 00:00:00
abstract：:Gregarines thrive in the digestive tract of arthropods and may be deleterious to their hosts, especially when present in high densities. The impact of parasites on these invertebrates may affect both the ecosystem equilibrium and human economic activities. However, information available on gregarines in Spain is limit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5266-0
更新日期：2017-01-01 00:00:00
abstract：:The diagnosis of toxoplasmosis is often complicated by the lack of specific clinical symptoms or postmortem features, in humans and other animals. The only diagnostic test described so far for the serological diagnosis of Toxoplasma gondii in marine mammals is the modified agglutination test (Dubey et al., Am J Vet Re...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3674-y
更新日期：2014-02-01 00:00:00
abstract：:Toxocara canis is an intestinal nematode of canids with a worldwide distribution, causing an important but neglected parasitic zoonosis in humans. Aquaporins (AQP) are a family of water channel proteins, which function as membrane channels to regulate water homeostasis. In this study, the coding sequence of aquaporin-...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5129-8
更新日期：2016-09-01 00:00:00
abstract：:The protozoan parasite Giardia duodenalis is known to infect humans and a wide range of animals globally. However, no studies on G. duodenalis infection in Bactrian camels have been reported. In the present study, in order to examine the prevalence and genetic diversity of G. duodenalis in Bactrian camels, 852 fecal s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06905-y
更新日期：2020-11-01 00:00:00
abstract：:Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to ce...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2045-1
更新日期：2011-01-01 00:00:00
abstract：:Blastocystis is an emerging zoonotic pathogen with global distribution. However, limited data exist on the prevalence and genetic diversity of Blastocystis in the USA and in food animals. We conducted the first large-scale molecular investigation of Blastocystis in the USA by testing 2539 fecal samples from dairy heif...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6149-3
更新日期：2019-02-01 00:00:00
abstract：:Visceral larva migrans (VLM) syndrome caused by Toxocara canis larvae was first described in the 1950s. The role of other nematode larvae, i.e. the pig roundworm Ascaris suum as a causative agent of visceral larva migrans-associated symptoms like general malaise, cough, liver dysfunction, hypereosinophilia with hepato...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4196-y
更新日期：2015-01-01 00:00:00
abstract：:A total of 116 samples (44 clinical specimens and 72 environmental samples) have been analyzed for the presence of Acanthamoeba. The environmental samples (ESs) were collected from four drinking water treatment plants (DWTP, n=32), seven wastewater treatment plants (n=28), and six locations of influence (n=12) on four...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2849-2
更新日期：2012-07-01 00:00:00
abstract：:Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5343-4
更新日期：2017-02-01 00:00:00
abstract：:Infection in dogs and humans with the protozoan parasite Leishmania are widespread in tropical and subtropical countries around the globe. Sand flies of the order Phlebotomus in the Old World and Lutzomyia in the New World function as the vector of this disease. In dogs, skin lesions are the most prominent signs of ca...
journal_title：Parasitology research
pub_type： 临床试验,杂志文章
doi：10.1007/s00436-003-0905-7
更新日期：2003-07-01 00:00:00
abstract：:The susceptibility of two Venezuelan (YT and SM) and one Brazilian (BH) strain of Schistosoma mansoni to single oral doses of praziquantel (Pz; 250 or 500 mg/kg), oxamniquine (Ox; 40, 60, or 100 mg/kg) or to low-dose combinations of both drugs (33 mg/kg Pz and 25 mg/kg Ox; 66 mg/kg Pz and 12.5 mg/kg Ox; 250 mg/kg Pz a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931515
更新日期：1992-01-01 00:00:00
