Abstract：
:A total of 116 samples (44 clinical specimens and 72 environmental samples) have been analyzed for the presence of Acanthamoeba. The environmental samples (ESs) were collected from four drinking water treatment plants (DWTP, n=32), seven wastewater treatment plants (n=28), and six locations of influence (n=12) on four river basins from the central area of Spain (winter-spring 2008). Water samples were concentrated by using the IDEXX Filta-Max(®) system. Acanthamoeba was identified in 65 of the 72 ESs by culture isolation (90.3%) and 63 by real-time PCR (87.5%), resulting in all sampling points (100%) positive for Acanthamoeba when considering both techniques and all the time period analyzed. Nine of the 44 clinical specimens were positive for Acanthamoeba. Seventeen Acanthamoeba strains (eight from four DWTP and nine from clinical samples) were also established in axenic-PYG medium. Twenty-four of the ESs and the 17 Acanthamoeba sp. strains were genotyped as T4/1, T4/8, and T4/9. The eight strains isolated from the DWTP samples were inoculated in nude mouse to ascertain their potential pathogenicity in this model. Animals that were inoculated died or showed central nervous system symptoms 9 days post-inoculation. Examination of immunofluorescence-stained brain and lung tissue sections showed multiple organisms invading both tissues, and re-isolation of throphozoites was successful in these tissues of all infected animals. For the first time, potentially pathogenic Acanthamoeba T4 has been detected in 100% of different types of water samples including tap water and sewage effluents in the central area of Spain suggesting a potential health threat for humans especially for the contact lens wearers.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Magnet A,Galván AL,Fenoy S,Izquierdo F,Rueda C,Fernandez Vadillo C,Pérez-Irezábal J,Bandyopadhyay K,Visvesvara GS,da Silva AJ,del Aguila Cdoi
10.1007/s00436-012-2849-2subject
Has Abstractpub_date
2012-07-01 00:00:00pages
383-92issue
1eissn
0932-0113issn
1432-1955journal_volume
111pub_type
杂志文章abstract：:A seasonal investigation on the occurrence of fishborne zoonotic trematodes (FZT) in economically important mono-cultured hybrid catfish and giant gouramy was conducted in the Mekong Delta of Vietnam. Fish from carp poly-culture and intensive small-scale integrated vegetable-aquaculture-animal husbandry farming (VAC) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0633-5
更新日期：2007-10-01 00:00:00
abstract：:We report the ultrastructural alterations induced on epimastigotes by nifurtimox and 5-nitro-2-thienyl-malononitrile (5NO2TM), a novel compound with anti-Trypanosoma cruzi activity. Parasites treated with concentrations of nifurtimox lower than usually employed for this kind of study showed vacuolisation, alterations ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100497
更新日期：2002-02-01 00:00:00
abstract：:A fundamental aim of parasite ecology is to understand the mechanisms behind spatial variation in diversity and structure of parasite assemblages. To understand the contribution of individual parasite species and their assemblages to spatial variation in parasite communities, we examined species contributions to beta ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06411-w
更新日期：2019-10-01 00:00:00
abstract：:In the present article, we report on the identification of Vermamoeba (Hartmannella) vermiformis as the etiological agent of a tissue infection close to the eye of a female patient. Laboratory examination revealed no involvement of any pathogenic bacteria or fungi in the tissue infection. V. vermiformis was identified...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06312-y
更新日期：2019-06-01 00:00:00
abstract：:Mosquito repellents represent a cleaner and safer alternative for population control and reduce the diseases they carry in large areas of the world. Recently, research has been focused on repellents of natural origins, both crude essential oils and their main constituents. We have observed that, although a large numbe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3827-7
更新日期：2014-05-01 00:00:00
abstract：:Metazoan parasites of guilds of benthic snails and crustaceans and of four fish families--Gobiidae, Gasterosteidae, Syngnathidae, and Zoarcidae--were investigated off the brackish Salzhaff area (Southwest Baltic) in the semienclosed Salzhaff and the near Rerik Riff in the free Baltic. Comparisons revealed greater simi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050681
更新日期：2000-05-01 00:00:00
abstract：:In the search of alternatives for the control of mosquitoes of medical importance, we evaluated the larvicidal activity of micronized suspensions of calcium hydroxide [Ca(OH)₂] against Aedes aegypti and Culex quinquefasciatus. Tests conducted under laboratory conditions determined a LC50 = 0.027% (LC90 = 0.096%) for A...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2593-z
更新日期：2012-03-01 00:00:00
abstract：:Because benznidazole (BZL) was found to downregulate nitric oxide (NO) and cytokine synthesis by murine macrophages, we analyzed the potential immunological repercussions of BZL treatment in Trypanosoma cruzi-infected rats. To evaluate whether the effects of BZL were also observed in the presence of an immunostimulati...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000357
更新日期：2001-07-01 00:00:00
abstract：:The essential oil of Cananga odorata flowers was evaluated for oviposition-deterrent, ovicidal, insecticidal, and repellent activities toward three mosquito species: Aedes aegypti, Anopheles dirus, and Culex quinquefasciatus. Oviposition deterrence of the oil was evaluated on gravid females using oviposition deterrenc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4699-1
更新日期：2015-12-01 00:00:00
abstract：:The oocysts of Caryospora cherrughi sp. n. were described from the feces of the saker falcon, Falco cherrug, collected from the falcon market in Riyadh City, Saudi Arabia. The prevalence of infection was 21.4 % (15/70). The majority of the oocysts examined had completed sporulation within 64-84 h at 24 ± 2 °C. Sporula...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3217-y
更新日期：2013-03-01 00:00:00
abstract：:Bats from the families Rhinolophidae (n = 90) and Vespertilionidae (n = 191) in the USA and Czech Republic were screened for the presence of Cryptosporidium by microscopic and molecular analysis of faecal samples collected from rectum of dissected animals and from the ground beneath roosting sites. Cryptosporidium ooc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4654-1
更新日期：2015-10-01 00:00:00
abstract：:Filter-feeding molluscan shellfish can concentrate environmentally derived waterborne pathogens of humans, which can be utilized in the sanitary assessment of water quality. In the present study, oocysts of Cryptosporidium were detected in Bent mussels (Ischadium recurvum) at two Chesapeake Bay sites from which C. par...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050590
更新日期：1999-07-01 00:00:00
abstract：:Lice infestations in horses caused by the chewing louse Werneckiella (Damalinia) equi are observed worldwide. In the present study, the efficacy of 10% imidacloprid was tested on horses naturally infested with lice. Two groups of animals received a double application of 4 ml and 8 ml Advantage 10% spot-on on day 0 and...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1379-6
更新日期：2005-08-01 00:00:00
abstract：:Groups of five rats each were infected with metacercariae of Fasciola hepatica according to two experimental procedures. In the first experiment, they received 20 metacercariae 0, 1, 2 or 3 times at intervals of 4 weeks. In the second, both the inoculation dose and the interval between doses were varied. After the ani...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931639
更新日期：1991-01-01 00:00:00
abstract：:Artesuante (AS) is a good chemoprophylactic drug for preventing against schistosome infection, but Hua et al. recently reported that the sensitivity of AS against Schistosoma japonicum decreased after 10 years of use in China. There are at least three problems, to our knowledge, making the finding suspicious or inconc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2641-8
更新日期：2012-04-01 00:00:00
abstract：:This paper follows the study from 2013 focused on the molecular screening of mosquitoes as vectors of Dirofilaria spp. which provided the information on Aedes vexans as a potential vector of Dirofilaria repens in Slovakia. Current entomological and molecular research indicates that Ae. vexans can participate also in t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4692-8
更新日期：2015-12-01 00:00:00
abstract：:Toxoplasma gondii is an opportunistic protozoan parasite that differentiates into latent cysts (bradyzoite) that can be reactivated during immunosuppression. TgSRCAP (Toxoplasma gondii Snf2-related CBP activator protein) is a SWI2/SNF2 family chromatin remodeler whose expression increases during cyst development. Iden...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1291-5
更新日期：2005-03-01 00:00:00
abstract：:In nature, parasitic infections must be addressed as complex systems involving parasite-host relationships on a temporal and spatial scale. Since the parasites cover a great biological diversity, we can expect that wildlife are exposed simultaneously to different parasites. In this sense, the objective of this work wa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-07028-0
更新日期：2021-01-09 00:00:00
abstract：:In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phag...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5175-2
更新日期：2016-10-01 00:00:00
abstract：:Sarcocysts were detected in routinely processed histological sections of skeletal muscle, but not cardiac muscle, of two adult male otters (Lutra lutra; Mustelidae) from northern Norway following their post-mortem examination in 1999 and 2000. The sarcocysts were slender, spindle-shaped, up to 970 μm long and 35-70 μm...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4251-8
更新日期：2015-03-01 00:00:00
abstract：:Differentiation between viable and non-viable hookworm ova in environmental samples is necessary in order to implement strategies to mitigate re-infections in endemic regions. In this study, an untargeted metabolic profiling method was developed that utilised gas chromatography-mass spectrometry (GC-MS) in order to in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5112-4
更新日期：2016-09-01 00:00:00
abstract：:Representatives of Apicomplexa perform various kinds of movements that are linked to the different stages of their life cycle. Ancestral apicomplexan lineages, including gregarines, represent organisms suitable for research into the evolution and diversification of motility within the group. The vermiform trophozoites...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06381-z
更新日期：2019-09-01 00:00:00
abstract：:In the present study, Diplectanum laubieri was redescribed from material obtained from wild and cultured fish. The population dynamics of D. aequans and D. laubieri under culture conditions was studied. The prevalence and mean intensity (average number of worms per parasitized fish) were 80.64% and 112 for D. aequans ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930906
更新日期：1991-01-01 00:00:00
abstract：:Sheared flagella from Giardia lamblia were freed from cytoskeleton fragments and other cell contaminants by centrifuging in a density gradient. The purified organelles contain many polypeptides, including a set of low-molecular-weight antigens [apparent molecular weights (MWs) = 31, 32, 34, 35 and 37 kD] in the same s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00535140
更新日期：1988-01-01 00:00:00
abstract：:Blastocystis is an enteric protozoan parasite commonly found in humans and animals. Phylogenetic and genotypic analyses have shown that Blastocystis exhibits extreme genetic diversity, and humans are host to a number of zoonotic isolates. In the present study, the prevalence of Blastocystis in 276 stool samples from a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0808-0
更新日期：2008-03-01 00:00:00
abstract：:This report describes for the first time the occurrence of alveolar echinococcosis in two exotic rodent species in Europe. A pet chinchilla (Chinchilla laniger) was euthanized due to a painful enlargement of the abdominal cavity, and a Prevost's squirrel (Callosciurus prevostii borneoensis) was found dead in the enclo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0717-2
更新日期：2007-11-01 00:00:00
abstract：:Leishmania (L.) tropica is a causative agent of cutaneous and occasionally visceral or viscerotropic leishmaniasis in humans. The dose of parasites influences the course and outcome of disease in some Leishmania species. The effect of parasite dose on L. tropica infection in an experimental model was studied in the cu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5991-7
更新日期：2018-09-01 00:00:00
abstract：:Infections with the metastrongyloid nematode Aelurostrongylus abstrusus in cats have been reported sporadically from Germany. To assess the occurrence and regional geographical distribution of A. abstrusus in Germany, faecal samples from 391 cats with symptoms of respiratory disease were collected from December 2009 t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3207-0
更新日期：2013-02-01 00:00:00
abstract：:The electron microscopic investigation of the anterior part of the infective third-stage juvenile of Onchocerca volvulus provides first insights into the structure of the excretory system of this developmental stage of the parasite. The most anterior part of this system consists of a cell process of the syncytial excr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931502
更新日期：1995-01-01 00:00:00
abstract：:Studies of the immune response in chronic helminth infections suggest that parasites modulate the host's immune response. Taenia solium metacestodes, in particular, produce molecules that down-regulate cell-mediated immunity. We have described a small RNA peptide termed metacestode factor (MF) that depresses the murin...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050367
更新日期：1998-01-01 00:00:00
