Abstract：
:Sheared flagella from Giardia lamblia were freed from cytoskeleton fragments and other cell contaminants by centrifuging in a density gradient. The purified organelles contain many polypeptides, including a set of low-molecular-weight antigens [apparent molecular weights (MWs) = 31, 32, 34, 35 and 37 kD] in the same size range as the approximately 30 kD structural giardins of the cytoskeleton. However, on sodium dodecyl sulphate-polyacrylamide gels, the mobilities of individual flagellar polypeptides do not correspond exactly to the cytoskeleton bands, and, unlike the cytoskeleton proteins, the flagellar components are easily extracted by Triton demembranation. The pattern of flagellar isoforms after isoelectric focussing (IEF) and electrophoresis in two dimensions is also clearly different from that of the cytoskeleton proteins. The fact that at least some approximately 30 kD flagellar antigens are localised by immunofluorescence specifically in the two ventral flagella suggests that these proteins may be components of the paraflagellar structures found beneath the membrane of these organelles. In electron micrographs of the isolated flagella, the paraflagellar rods are seen to bridge the membrane to three adjacent doublet microtubules of the axoneme.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Clark JT,Holberton DVdoi
10.1007/BF00535140subject
Has Abstractpub_date
1988-01-01 00:00:00pages
415-23issue
5eissn
0932-0113issn
1432-1955journal_volume
74pub_type
杂志文章abstract：:Toxoplasma gondii is of public health and veterinary importance causing severe diseases in immunocompromised individuals including HIV/AIDS patients and in congenital cases and animals. There is limited information on the epidemiology of T. gondii infection in humans, particularly HIV patients and food animals and the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5442-x
更新日期：2017-06-01 00:00:00
abstract：:Cyclospora cayetanensis was identified as the cause of large outbreaks of diarrhea in many parts of the world, but its host range and reservoirs remains poorly defined. Recently, oocysts resembling the C. cayetanensis were detected in dairy cattle fecal specimens from China. The 18S rDNA from two of these Cyclospora-l...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0380-z
更新日期：2007-04-01 00:00:00
abstract：:The present study aimed to isolate and genotype strains of T. gondii from pigs slaughtered for human consumption in South Brazil. Blood and tissues (heart, diaphragm, liver, tongue, and masseter) from 400 animals were collected at two slaughterhouses. Sera were obtained, and antibodies against T. gondii were detected ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06274-1
更新日期：2019-05-01 00:00:00
abstract：:Paramyosin is a muscle protein that probably plays a role in the survival of the larval stage of Taenia solium during its prolonged host-parasite relationship. Here we describe the structure of the gene coding for the paramyosin of T. solium. The characterization of two clones obtained from a genomic library showed th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0761-x
更新日期：2003-03-01 00:00:00
abstract：:Necrophagous Sarcophagidae are among the insects most frequently reported from human corpses. The broad forensic application of flesh flies is restricted by the lack of reliable tools for species identification of larval stages and mass breeding of collected flesh fly larvae to the adult stage, and more recently DNA-b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4421-3
更新日期：2015-06-01 00:00:00
abstract：:Amastigotes of Leishmania species belonging to the Leishmania mexicana complex exhibit large lysosomes, called megasomes, which are rich in cysteine proteinases. Various aspects of the host-parasite interaction, the differentiation process as well as intracellular survival, have been attributed to these proteinases. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-001-0516-0
更新日期：2002-04-01 00:00:00
abstract：:Specific anti-newborn larva antibodies present in the serum of rats chronically infected with Trichinella spiralis were incapable of inducing killing of newborn larvae by activation of normal peritoneal cells. These late antibodies blocked the cytotoxic reaction induced by early antibodies produced a few weeks after i...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050072
更新日期：1996-01-01 00:00:00
abstract：:During investigations of fish parasites in the Neotropics (including the state of Veracruz and the Yucatán Peninsula in the Gulf of Mexico, the Chautengo Lagoon on the Pacific coast of the state of Guerrero in Mexico, and Lake Gatun in the Panama Canal), three monogenoidean (Dactylogyridae) species were found parasiti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1442-9
更新日期：2009-09-01 00:00:00
abstract：:Diagnostic parameters for visceral leishmaniasis (VL), a potentially fatal parasitic disease caused by Leishmania donovani, have been redefined in the last decade with the development of serological and molecular tests, though a definitive diagnosis still banks on the century-old parasitological methods in many areas....
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-011-2680-1
更新日期：2012-03-01 00:00:00
abstract：:A total of 585 persons from several occupational groups (farmers, slaughterhouse staff, hunters, veterinarians) exposed to Toxocara infestations and 50 persons of a control group were tested for the presence of specific antibodies to the Toxocara canis antigen using an enzyme-linked immunosorbent assay and a western b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1469-5
更新日期：2005-11-01 00:00:00
abstract：:Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050419
更新日期：1998-05-01 00:00:00
abstract：:The bacteria Anaplasma platys, Anaplasma phagocytophilum and Ehrlichia canis are tick-borne agents that cause canine vector-borne disease. The prevalence of these pathogens in South Eastern Europe is unknown with the exception of an isolated case of A. platys detected in a dog imported into Germany from Croatia. To ga...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5611-y
更新日期：2017-11-01 00:00:00
abstract：:During a parasitological survey of two non-native fish species-racer goby Neogobius gymnotrachelus and monkey goby Neogobius fluviatilis-in the Włocławek Reservoir on the lower Vistula River, the monogenean Gyrodactylus proterorhini was recorded for the first time in Poland, and for the first time, the racer goby was ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2175-5
更新日期：2011-05-01 00:00:00
abstract：:Ivermectin at single doses of 0.2-1.0 mg/kg body weight reduced the microfilarial counts of subperiodic Brugia malayi in Presbytis cristata by 59.9%-89.6% of initial counts, 4 weeks after treatment. Adult filaricidal activity was poor, live adult worms being recovered from all animals at autopsy. There was no serious ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00539462
更新日期：1988-01-01 00:00:00
abstract：:Fecal specimens from 432 pre-weaned calves younger than 35 days were collected over a 2-year period (2010-2012) from 74 dairy cattle farms in the central area of Colombia. These samples were microscopically examined for the presence of Cryptosporidium oocysts, and positive specimens were selected for molecular examina...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5818-6
更新日期：2018-05-01 00:00:00
abstract：:Studies of the immune response in chronic helminth infections suggest that parasites modulate the host's immune response. Taenia solium metacestodes, in particular, produce molecules that down-regulate cell-mediated immunity. We have described a small RNA peptide termed metacestode factor (MF) that depresses the murin...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050367
更新日期：1998-01-01 00:00:00
abstract：:The aim of this study was to evaluate the acaricidal activity on the cattle tick Rhipicephalus (Boophilus) microplus of essential oils from three Ocimum species. Acaricidal activity of five essential oils extracted from Ocimum gratissimum L. (three samples), O. urticaefolium Roth, and O. canum Sims was evaluated on 14...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4164-6
更新日期：2015-01-01 00:00:00
abstract：:We report on serine protease activity in cercarial secretions (CSs) from the bird parasite Trichobilharzia ocellata. Using a colorigenic substrate, the biochemical properties of this enzyme were studied and its activity was compared to the homologous one in CSs from the human parasite Schistosoma mansoni. The specific...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0597-4
更新日期：2002-06-01 00:00:00
abstract：:Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2898-6
更新日期：2012-09-01 00:00:00
abstract：:In recent years, the mitochondrial haplotype diversity of the monogenean ectoparasites Gyrodactylus salaris Malmberg, 1957 on Atlantic salmon and G. thymalli Zitnan, 1960 on grayling has been studied intensively to understand the taxonomy and phylogeography of the two species. According to these studies, neither speci...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0423-5
更新日期：2007-05-01 00:00:00
abstract：:The pathogenicity of 19 hypocrealean entomopathogenic fungi from seven different genera in adult Aedes aegypti was tested. All fungi proved to be pathogenic, and Isaria fumosorosea, Lecanicillium muscarium, Lecanicillium psalliotae, Metarhizium anisopliae, Metarhizium lepidiotae, Metarhizium majus, Metarhizium frigidu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1991-y
更新日期：2010-10-01 00:00:00
abstract：:Pharmacokinetics and anthelmintic activity of topical eprinomectin in goats prevented from physical contact to others and self-grooming were studied. Sixteen approximately 7 months old male castrated German White Noble goats harbouring induced infections of gastrointestinal nematode parasites were included in the stud...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4072-9
更新日期：2014-11-01 00:00:00
abstract：:The ultrastructure of the tegument, including putative subtegumental cells, of the monogenean gill parasite Macrogyrodactylus clarii is described for the first time. The lateral cells are secretory in nature and can be classified into four types: T1 producing elongated, electron-dense, bodies (t1); T2 manufacturing el...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0448-9
更新日期：2007-06-01 00:00:00
abstract：:This study aims to observe the histopathological alterations in schistosomula of Schistosoma japonicum induced by mefloquine. Mice were infected with S. japonicum cercariae, and after 14 days, a single dose of mefloquine (400 mg/kg) was administered orally. After 8 h, 24 h, 3 days, 7 days, and 14 days, groups of two t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1611-x
更新日期：2009-11-01 00:00:00
abstract：:Lymphatic filariasis is mainly caused by the filarial nematodes Wuchereria bancrofti and Brugia malayi. Wolbachia, intracellular symbiotic bacteria in filarial parasite, is known to induce immune response predominantly through Toll-like receptor 2 (TLR2). This study was performed to investigate the association between...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1932-9
更新日期：2010-09-01 00:00:00
abstract：:Malaria is caused by unicellular parasites of the genus Plasmodium, which reside in erythrocytes during the clinically relevant stage of infection. To separate parasite from host cell material, haemolytic agents such as saponin are widely used. Previous electron microscopy studies on saponin-treated parasites reported...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06932-9
更新日期：2020-12-01 00:00:00
abstract：:When used at low concentrations and added to the water body, water-soluble chlorophyllin (resulting from chlorophyll after removal of the phytol) and pheophorbid (produced from chlorophyllin by acidification) are able to kill mosquito larvae and other small animals within a few hours under exposure of solar radiation....
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1235-6
更新日期：2009-02-01 00:00:00
abstract：:The objective of the study was to estimate the prevalence of helminths in the horse population of the state of Brandenburg, Germany. One hundred and twenty-six horse farms in the state were selected by randomised stratified sampling. In total, 1,407 horses across all farms were examined coproscopically. The experiment...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2362-z
更新日期：2011-05-01 00:00:00
abstract：:There appear to be few reports in the literature regarding the host-poultry red mite (Dermanyssus gallinae) immunological relationship, despite the negative impact D. gallinae can have on both bird welfare and egg production, as well as its potential to act as a reservoir of zoonotic infections. The current study inve...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1821-2
更新日期：2010-05-01 00:00:00
abstract：:Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to ce...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2045-1
更新日期：2011-01-01 00:00:00
