Molecular characterization of Cyclospora-like organism from dairy cattle.

Abstract:

:Cyclospora cayetanensis was identified as the cause of large outbreaks of diarrhea in many parts of the world, but its host range and reservoirs remains poorly defined. Recently, oocysts resembling the C. cayetanensis were detected in dairy cattle fecal specimens from China. The 18S rDNA from two of these Cyclospora-like oocyst specimens from dairy cattle was amplified and sequenced. Phylogenetic analysis indicated that these cattle-associated Cyclospora-like organisms are nearly identical to each other and belong to the group of primate-derived Cyclospora, which are the closest known relatives of C. cayetanensis; while these cyclosporans constitute a coherent clade within the diverse group of Eimeria species. Moreover, on the basis of our finding that ruminant- and avian-associated Eimeria species are different in MnlI sites, a new PCR-restriction fragment length polymorphism protocol with primers NesCycF and NesCycR was developed to distinguish the Cyclospora species from ruminant-associated Eimeria species.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Li G,Xiao S,Zhou R,Li W,Wadeh H

doi

10.1007/s00436-006-0380-z

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

955-61

issue

5

eissn

0932-0113

issn

1432-1955

journal_volume

100

pub_type

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