Abstract:
:Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a more detailed analysis. The p67 and p104 antigens were present only in the sporozoite lysates, whereas PIM and p150 were found in both sporozoite and schizont lysates. The four antigens were expressed as recombinant fusion proteins and were compared with each other in an enzyme-linked immunosorbent assay (ELISA) and in the whole-schizont-based indirect fluorescent antibody test (IFAT) in terms of their ability to detect antibodies in sera of experimentally infected cattle. The PIM-based ELISA provided a higher degree of sensitivity and specificity than did the ELISA using the other three recombinant antigens or the IFAT. Further evaluation of the PIM-ELISA using experimental sera derived from cattle infected with different hemoparasites and field sera from endemic and nonendemic T. parva areas showed that the assay had a sensitivity of > 99% and a specificity of between 94% and 98%.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Katende J,Morzaria S,Toye P,Skilton R,Nene V,Nkonge C,Musoke Adoi
10.1007/s004360050419subject
Has Abstractpub_date
1998-05-01 00:00:00pages
408-16issue
5eissn
0932-0113issn
1432-1955journal_volume
84pub_type
杂志文章abstract::Infectious agents, including parasites, often have oncogenic potential. However, there has been no study on the association between Strongyloides stercoralis infection and cancer risk. Therefore, we investigated the relationship between S. stercoralis infection and the occurrence of hepato-pancreato-biliary cancer. Th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0648-y
更新日期:2007-10-01 00:00:00
abstract::There appear to be few reports in the literature regarding the host-poultry red mite (Dermanyssus gallinae) immunological relationship, despite the negative impact D. gallinae can have on both bird welfare and egg production, as well as its potential to act as a reservoir of zoonotic infections. The current study inve...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1821-2
更新日期:2010-05-01 00:00:00
abstract::Capillaria hepatica (syn. Calodium hepaticum) is a globally distributed nematode with a high affinity to the liver of a wide range of mammalian hosts, including humans. Documented reports of the nematode in cats and associated histopathology are rare. Here, we describe a case of C. hepatica infection in a 5-year-old m...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-021-07056-4
更新日期:2021-01-22 00:00:00
abstract::Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukar...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1742-0
更新日期:2010-04-01 00:00:00
abstract::Our aim was to study the migration of retinal pigmented epithelium (RPE) into the retinal layer during infection of C57BL/6 mice with Toxoplasma gondii. Eyes from infected and non-infected animals were analyzed on the 60th day of infection by light and transmission electron microscopy. Non-infected eyes showed a norma...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1031-2
更新日期:2004-04-01 00:00:00
abstract::Legionella were detected with the direct DNA extraction method, Legionella culture method, and free-living amoebae (FLA) culture method from weak alkaline carbonate spring water in Taiwan. Moreover, we also investigated the existence of Acanthamoeba, Hartmannella, and Naegleria, ubiquitous FLA in aquatic environments,...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2366-8
更新日期:2011-11-01 00:00:00
abstract::This study was conducted to evaluate the phytochemical composition and larvicidal effect of leaf essential oil from Murraya exotica against early fourth-instar larvae of Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. Gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS) analyses reve...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4370-x
更新日期:2015-05-01 00:00:00
abstract::When used at low concentrations and added to the water body, water-soluble chlorophyllin (resulting from chlorophyll after removal of the phytol) and pheophorbid (produced from chlorophyllin by acidification) are able to kill mosquito larvae and other small animals within a few hours under exposure of solar radiation....
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1235-6
更新日期:2009-02-01 00:00:00
abstract::A multicomponent DNA vaccine, encoding Toxoplasma gondii GRA1 and SAG1, was constructed and tested for its ability to confer protection. BALB/c mice were challenged with tachyzoites of the virulent T. gondii RH strain at 4 weeks following the last immunization, and immune responses and survival times were observed. Th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-3047-y
更新日期:2012-11-01 00:00:00
abstract::Attractive toxic sugar bait (active ingredient, 1% boric acid) was evaluated against Aedes albopictus Skuse populations in the laboratory, semi-field trials, and field trials in residential communities in St. Augustine, Florida. Laboratory evaluations of boric acid sugar baits applied to the plant Pentas lanceolata (R...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3312-8
更新日期:2013-04-01 00:00:00
abstract::We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyp...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1304-x
更新日期:2009-03-01 00:00:00
abstract::The recent resurgence of the common bed bug Cimex lectularius L. throughout western industrialized nations has been facilitated in part by the insect becoming pesticide-resistant. Novel control strategies, including xenointoxication, should be considered to combat C. lectularius. Ivermectin, a U.S. Food and Drug Admin...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5062-x
更新日期:2016-08-01 00:00:00
abstract::Anopheles (Cellia) stephensi Liston 1901 is one of the major malaria vectors in the Indian subcontinent, Iran, and the Middle East. Three races in this species, namely A. stephensi stephensi (type form), A. stephensi variety mysorensis, and A. stephensi intermediate form, have earlier been reported by several investig...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-0930-7
更新日期:2008-06-01 00:00:00
abstract::The gullet worm (Gongylonema pulchrum) has been recorded from a variety of mammals worldwide. In an earlier study, we demonstrated two separate transmission cycles in cattle (Bos taurus) and wild mammals in Japan based on nucleotide sequences of the ribosomal RNA gene (rDNA) and cytochrome c oxidase subunit I (cox-1) ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4806-3
更新日期:2016-02-01 00:00:00
abstract::In Europe, mosquito-related public health concerns are growing due to the increasing spread of invasive mosquito species and the recent emergence of mosquito-borne arboviruses. A vital backbone in the assessment of these issues is detailed knowledge of the mosquito fauna, i.e. regional mosquito inventories. It was the...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-3952-3
更新日期:2014-08-01 00:00:00
abstract::Interferon-tau (IFN-tau) treatment of Trichinella spiralis-infected BALB/c mice was investigated. The therapeutic regimen consisted of daily intraperitoneal injection of 10(4) U murine IFN-tau for 7 days, starting at 2 weeks post-infection. Striated muscle samples (diaphragm, thigh) were collected at 4, 8 and 12 weeks...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931641
更新日期:1991-01-01 00:00:00
abstract::The taxonomy and classification of the family Opisthorchiidae have been revised by several authors with the exclusion or synonymization of some genera. The genus Hepatiarius Feizullaev, 1961 accommodated two species: Hepatiarius sudarikovi Feizullaev, 1961 and H. longissimus Linstow, 1883. Recently, some experts have ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06227-8
更新日期:2019-03-01 00:00:00
abstract::The time-course of the detection of circulating and urinary filarial antigens was followed with a 2S-IRMA assay, using a mouse monoclonal antibody raised against Brugia malayi larvae, in Mastomys natalensis experimentally infected with Brugia malayi, Brugia pahangi, or Litomosoides carinii. In the prepatent phase of t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00536033
更新日期:1987-01-01 00:00:00
abstract::Cryptosporidium spp. are common intestinal protozoa causing diarrhea in humans and a variety of animal species. With the recent development of fur industry, a large number of fur animals are farmed worldwide, especially in China. The existence of identical Cryptosporidium species/genotypes in humans and fur animals su...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5686-5
更新日期:2018-01-01 00:00:00
abstract::Ticks and tick-borne diseases (TBDs) continue to pose an insidious and ever-present threat to livestock and livelihoods across the globe. Two of the most significant TBDs of cattle in Africa are heartwater and babesioisis, caused by Ehrlichia ruminantium and Babesia bigemina respectively. Both pathogens are endemic in...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06204-1
更新日期:2019-03-01 00:00:00
abstract::This study aimed to investigate the links between parasites and behavioural traits of juvenile Murray cod (Maccullochella peelii). The Murray cod is an endangered Australian freshwater fish for which restocking programs are in place and there is a growing human consumption market. However, little is known about the pa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-07021-7
更新日期:2021-01-06 00:00:00
abstract::The effect of pentavalent meglumine antimoniate (glucantime) on the growth kinetics of promastigotes of 13 South American Leishmania strains isolated from patients, sylvatic reservoirs, and vectors was studied. Four of five L. (Viannia) braziliensis human isolates were obtained from drug-responsive patients and one wa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050476
更新日期:1998-09-01 00:00:00
abstract::Sera of ten dogs infected with Angiostrongylus vasorum were analyzed by immunoblotting proteins of first stage larvae (L1) and adult parasites. The molecular weights (m.w.) of the principal L1 proteins identified by IgG, IgM, IgA, and IgE were 18-118 kDa and those of the adult parasite were 28-209 kDa. The L1 proteins...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0774-6
更新日期:2008-02-01 00:00:00
abstract::During a parasitological survey of two non-native fish species-racer goby Neogobius gymnotrachelus and monkey goby Neogobius fluviatilis-in the Włocławek Reservoir on the lower Vistula River, the monogenean Gyrodactylus proterorhini was recorded for the first time in Poland, and for the first time, the racer goby was ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2175-5
更新日期:2011-05-01 00:00:00
abstract::Infections of Eimeria tenella in chicken embryos were used to compare the anticoccidial activity of ten drugs. The minimal inhibitory concentration (MIC) and minimal toxic concentration (MTC) were affected by the time of inoculation into the embryos and by the chemical nature of the compounds. Some compounds (nicarbaz...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931020
更新日期:1991-01-01 00:00:00
abstract::Avian malaria is of significant ecological importance and serves as a model system to study broad patterns of host switching and host specificity. The erythrocyte invasion mechanism of the malaria parasite Plasmodium is mediated, in large part, by proteins of the erythrocyte-binding-like (ebl) family of genes. However...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-3211-4
更新日期:2013-03-01 00:00:00
abstract::A 1-month-old brown wood owlet (Strix leptogrammica) purchased from a wholesaler and housed as a companion bird by an individual owner in Japan showed severe dehydration and anorexia following a week of vomiting and severe diarrhea. A great number of approximately 5 × 4-μm-sized Cryptosporidium oocysts were found in t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5951-2
更新日期:2018-09-01 00:00:00
abstract::The metacercarial cyst walls of Paragonimus westermani, P. miyazakii, P. ohirai, and P. iloktsuenensis were examined using ultrastructural and histochemical techniques. The cyst walls of P. westermani, P. miyazakii, and P. ohirai were found to have five distinct layers, whereas the wall of P. iloktsuenensis had only t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931563
更新日期:1992-01-01 00:00:00
abstract::Experimental infections of Galba truncatula with Fasciola hepatica were carried out under laboratory conditions to determine why the use of Tetraphyll, when provided every 3 days as food for snails, stimulated the intensity of cercarial shedding. In snails raised on cos lettuce and Tetraphyll, the number of metacercar...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1172-y
更新日期:2004-09-01 00:00:00
abstract::Triatoma platensis is occasionally found coexisting with Triatoma infestans in chicken coops in Argentina. Some authors have reported the presence of hybrid specimens of both species in chicken coops and other peridomestic habitats. Given the coexistence of T. infestans with T. platensis and the possibility of generat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06822-0
更新日期:2020-09-01 00:00:00