Abstract：
:Malaria is caused by unicellular parasites of the genus Plasmodium, which reside in erythrocytes during the clinically relevant stage of infection. To separate parasite from host cell material, haemolytic agents such as saponin are widely used. Previous electron microscopy studies on saponin-treated parasites reported both, parasites enclosed by the erythrocyte membrane and liberated from the host cell. These ambiguous reports prompted us to investigate haemolysis by live-cell time-lapse microscopy. Using either saponin or streptolysin O to lyse Plasmodium falciparum-infected erythrocytes, we found that ring-stage parasites efficiently exit the erythrocyte upon haemolysis. For late-stage parasites, we found that only approximately half were freed, supporting the previous electron microscopy studies. Immunofluorescence imaging indicated that freed parasites were surrounded by the parasitophorous vacuolar membrane. These results may be of interest for future work using haemolytic agents to enrich for parasite material.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Quadt KA,Smyrnakou X,Frischknecht F,Böse G,Ganter Mdoi
10.1007/s00436-020-06932-9subject
Has Abstractpub_date
2020-12-01 00:00:00pages
4297-4302issue
12eissn
0932-0113issn
1432-1955pii
10.1007/s00436-020-06932-9journal_volume
119pub_type
杂志文章abstract：:Mastomys coucha and jirds infected with Acanthocheilonema viteae, a filarial species free of endosymbiontic bacteria of the genus Wolbachia, suffer lethal side effects after effective microfilaricidal therapy with diethylcarbamazine and levamisole, whereas, M. coucha infected with the Wolbachia-infested species Brugia...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1386-7
更新日期：2005-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0808-0
更新日期：2008-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3138-9
更新日期：2013-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0851-x
更新日期：2008-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06287-w
更新日期：2019-05-01 00:00:00
abstract：:Ruminant coccidiosis is a gastrointestinal disease caused by parasites of the genus Eimeria. Environmental and climatic factors are relevant for the development, survival, and transmission of coccidiosis because Eimeria oocysts are able to survive in the environment for several weeks or months in favorable conditions ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06548-8
更新日期：2020-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4973-x
更新日期：2016-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050184
更新日期：1996-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2550-x
更新日期：2012-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5757-2
更新日期：2018-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0261-5
更新日期：2007-01-01 00:00:00
abstract：:Blastocystis is a zoonotic protozoan parasite frequently identified in the intestinal tract of humans and a vast variety of animals, worldwide. Here, we assessed the prevalence of Blastocystis and its subtypes in stool samples of raccoons. Stool samples from 30 raccoons were collected. Total DNA was extracted, and the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06770-9
更新日期：2020-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0151-x
更新日期：2006-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050062
更新日期：1996-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931216
更新日期：1993-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0642-3
更新日期：2002-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2552-8
更新日期：2012-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2427-z
更新日期：2011-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06328-4
更新日期：2019-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5062-x
更新日期：2016-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06699-z
更新日期：2020-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3444-x
更新日期：2013-07-01 00:00:00
abstract：:Plasmodium falciparum malaria in pregnancy is a major public health problem in sub-Saharan Africa and represents enormous diagnostic challenge. The objective of this report was to review scientific data from studies conducted in sub-Saharan Africa on the diagnosis of malaria in pregnancy within the last two decades (1...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0782-6
更新日期：2008-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1611-x
更新日期：2009-11-01 00:00:00
abstract：:A high-resolution melting (HRM) assay was applied to distinguish between Giardia duodenalis assemblages A and B from human and dog feces based on the triosephosphate isomerase gene (tpi). The genomic DNAs were selected from assemblages A (WB) and B (GS) as reference and plasmids were constructed. The reference plasmid...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3064-x
更新日期：2012-11-01 00:00:00
abstract：:Intestinal coccidiosis of rabbits induced by E. coecicola causes enormous economic losses in rabbit farms. Here, we investigate the effect of E. coecicola on the liver of the rabbit Oryctolagus cuniculus. On day 7 p.i., fecal expulsion of E. coecicola oocysts is maximal and rabbits have lost approximately 25% of their...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2574-2
更新日期：2012-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5861-3
更新日期：2018-06-01 00:00:00
abstract：:Cattle tick (Rhipicephalus microplus) is an important constraint on livestock production, particularly in tropical and subtropical areas. Synthetic acaricides are the primary method of control this thick. Resistance of R. microplus to synthetic acaricides has given rise to the need of new scientific investigations on ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5425-y
更新日期：2017-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06229-6
更新日期：2019-04-01 00:00:00
abstract：:Saliva has been suggested as an easily accessible and a noninvasive diagnostic alternative for detection of antibodies. To identify and characterize Schistosoma japonicum (S. japonicum) antigens that are recognized by saliva of infected host, we have used a pool of saliva from infected patients to immunoscreen an egg ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1575-x
更新日期：2009-10-01 00:00:00
