Abstract：
:High levels of lactate dehydrogenase (LDH; EC 1. 1. 1. 27) activity have been detected in the filarial worm Molinema dessetae. The two major LDH isoenzymes (LDH1 and LDH2) from female worms were purified by successive chromatography on diethylaminoethyl (DEAE)-Sepharose, carboxymethyl (CM)-Sepharose, and hydroxyapatite columns followed by fast protein liquid chromatography (FPLC)-gel filtration. LDH1 and LDH2 isoenzymes were found to be dimers with subunits of 58 kDa. They had similar properties with regard to substrate and coenzyme affinity. The apparent Michaelis constants (K(m) values; mean +/- SEM, n = 10) were 0.34 +/- 0.04 mM for pyruvate, 0.25 +/- 0.02 mM for reduced nicotinamide adenine dinucleotide (NADH), 2.5 +/- 0.21 mM for lactate, and 0.18 +/- 0.02 mM for NAD, which suggested that pyruvate reduction was the favored reaction. LDH1 and LDH2 were affected by p-chloromercuribenzoate and Hg2+, and such inhibitory effects could be reversed by the addition of thiol compounds (L-cysteine or beta-mercaptoethanol) as observed for mammalian LDH. Oxalate acted as a noncompetitive inhibitor of pyruvate reduction (Ki = 4.7 +/- 0.35 mM; mean +/- SEM, n = 10) and as a competitive inhibitor with lactate (Ki = 2.3 +/- 0.21 mM), whereas oxamate acted as a competitive inhibitor with pyruvate (Ki = 3.3 +/- 0.28 mM) and was noncompetitive with lactate (Ki = 19 +/- 1.2 mM). These substrate analogues exerted similar effects on mammalian LDH, but the inhibition constants were significantly different. The existence of structural and kinetic differences between mammal and filarial LDH isoenzymes prompted us to evaluate them as targets for chemotherapeutic attack.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Marchat L,Loiseau PM,Petek Fdoi
10.1007/s004360050184subject
Has Abstractpub_date
1996-01-01 00:00:00pages
672-80issue
8eissn
0932-0113issn
1432-1955journal_volume
82pub_type
杂志文章abstract：:Supplementary feeding is widely used in game management but may aid the transmission of parasites. Firstly, feeding sites attract animals and may be regarded as high-risk areas for parasite transmission. Secondly, high host population densities resulting from and supported by supplementary feeding, as well as accumula...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5512-0
更新日期：2017-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2275-x
更新日期：2011-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0124-5
更新日期：2006-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931537
更新日期：1995-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4649-y
更新日期：2015-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100497
更新日期：2002-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931823
更新日期：1993-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1352-x
更新日期：2009-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-0996-1
更新日期：2004-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050467
更新日期：1998-08-01 00:00:00
abstract：:Toxoplasma gondii is an opportunistic protozoan parasite that differentiates into latent cysts (bradyzoite) that can be reactivated during immunosuppression. TgSRCAP (Toxoplasma gondii Snf2-related CBP activator protein) is a SWI2/SNF2 family chromatin remodeler whose expression increases during cyst development. Iden...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1291-5
更新日期：2005-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0810-6
更新日期：2008-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0396-4
更新日期：2007-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100433
更新日期：2001-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00933778
更新日期：1994-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0092-9
更新日期：2006-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-07028-0
更新日期：2021-01-09 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3497-x
更新日期：2013-09-01 00:00:00
abstract：:Nitric oxide (NO) production by peritoneal macrophages was evaluated in Calomys callosus and Swiss mice during the course of infection with two strains of Trypanosoma cruzi. In C. callosus, no NO production was detected throughout the period of observation in animals infected with either parasite strain, except for a ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050336
更新日期：1997-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,评审
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更新日期：2009-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00436-010-2114-5
更新日期：2011-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1333-7
更新日期：2005-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050681
更新日期：2000-05-01 00:00:00
abstract：:With the advent of polymerase chain reaction (PCR), genetic markers are now accessible for all organisms, including parasites. Amplified fragment length polymorphism (AFLP) is a PCR-based marker for the rapid screening of genetic diversity and intraspecific variation. It is a potent fingerprinting technique for genomi...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-012-3238-6
更新日期：2013-02-01 00:00:00
abstract：:A LightCycler real-time polymerase chain reaction (PCR) assay was developed to detect Wuchereria bancrofti DNA in blood-fed mosquitoes. The assay is based on fluorescence melting curve analysis of the PCR product generated from a family of repeated DNA elements: the 182 bp SspI repeat, specific to the genus Wuchereria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1221-6
更新日期：2004-11-01 00:00:00
abstract：:Molecular karyotypes that revealed size polymorphisms between homologous forms of chromosomes 1, 2, 3 or 4 in three strains of the coccidial parasite Eimeria tenella were derived by pulsed field gel electrophoresis (PFGE) using brands of agaroses marketed either specifically for PFGE or for purposes such as protein el...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0775-4
更新日期：2003-03-01 00:00:00
abstract：:A molecular phylogenetic hypothesis is presented for the genus Trichuris based on sequence data from mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob). The taxa consisted of nine populations of whipworm from five species of Sigmodontinae rodents from Argentina. Bayesian Inference, Maximum Parsimony, a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5045-y
更新日期：2016-08-01 00:00:00
abstract：:The uptake of amino acids is mediated by active transporters located on the basolateral and brush border membranes of intestinal epithelial cells. The current study investigated the expression of amino acid transporters (AAT) and other genes in the intestine of chicks infected with Eimeria maxima. At 7-day postinfecti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4114-3
更新日期：2014-10-01 00:00:00
abstract：:Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3262-6
更新日期：2013-05-01 00:00:00
abstract：:We investigated the prevalence and genotypes of Cryptosporidium parasite in 50 brown rats (Rattus norvegicus) inhabiting an urban area of Japan. Fecal samples collected from the animals were examined by an immuno-fluorescence assay (IFA). Genomic DNA was extracted directly from fecal sample of each animal and nested P...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0488-9
更新日期：2007-05-01 00:00:00
