Abstract:
:A post-embedding immunogold technique was used to examine the subcellular distribution of immunoreactivities to the invertebrate peptide, FMR-Famide, and to vertebrate pancreatic polypeptide (PP) within the central nervous system of the trematode, Fasciola hepatica. Gold labeling of peptide was localised exclusively over both dense-cored and ellipsoidal electron-dense vesicles (with a homogeneous matrix) present within nerve cell bodies, small and 'giant' nerve processes of the neuropile in the cerebral ganglia and transverse commissure, as well as in the main longitudinal nerve cords. Double labeling demonstrated an apparent co-localisation of FMRFamide and PP immunoreactivities in the same dense-cored vesicles, although populations of ellipsoidal electron-dense vesicles that labeled solely for FMRFamide were also evident. Antigen pre-absorption studies indicated little, if any, cross-reactivity of the two antisera.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Brownlee DJ,Brennan GP,Halton DW,Fairweather I,Shaw Cdoi
10.1007/BF00933778subject
Has Abstractpub_date
1994-01-01 00:00:00pages
117-24issue
2eissn
0932-0113issn
1432-1955journal_volume
80pub_type
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journal_title:Parasitology research
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