Abstract：
:The catalysis of malate dehydrogenase (MDH) in Plasmodium falciparum (pfMDH) which involves NAD/NADH coupling is crucial for the parasite's pathogenicity. Primers were designed based on the P. falciparum genome resource, and these facilitated the cloning of a gene coding for pfMDH from a local clinical isolate. The DNA sequence of the cloned gene revealed an open-reading frame that encodes a protein of 313 amino acids. After induction in Escherichia coli BL21, enzyme assays of the expressed pfMDH purified by affinity chromatography exhibited significant enzyme activity of about 50 U/mg, where one unit (U) of enzyme activity is defined as the amount of enzyme oxidising 1 microol NADH/min. Based on its phylogenetic status amongst MDHs and lactate dehydrogenases (LDHs), the cloned gene was clearly defined as belonging to the NADH-dependent [LDH-like] MDHs. It is noteworthy that pfMDH harbours unique structural characteristics potentially useful for screening drugs specific for disabling parasitic enzymes.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Chan M,Sim TSdoi
10.1007/s00436-003-0996-1subject
Has Abstractpub_date
2004-01-01 00:00:00pages
43-7issue
1eissn
0932-0113issn
1432-1955journal_volume
92pub_type
杂志文章abstract：:Plasmodium falciparum (Pf) refurbishes the infected erythrocytes by exporting a myriad of parasite proteins to the host cell. A novel exported protein family 'Plasmodium Helical Interspersed Subtelomeric' (PHIST) has gained attention for its significant roles in parasite biology. Here, we have collected and analysed a...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-019-06420-9
更新日期：2019-10-01 00:00:00
abstract：:This randomised controlled laboratory study demonstrated the residual speed of efficacy of an imidacloprid/flumethrin collar (Seresto(®), Bayer) for the control of ticks (Dermacentor variabilis, Amblyomma americanum) at 6 and 12 hours postinfestation on dogs when compared to oral afoxolaner (NexGard(®), Merial). Dogs ...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-015-4515-y
更新日期：2015-08-01 00:00:00
abstract：:A case-control study was designed to investigate the role of different Cryptosporidium spp. in Swedish dairy herds with and without calf diarrhoeal problems. Faecal samples were collected from preweaned calves, young stock and cows. Cryptosporidium oocysts were detected by sodium chloride flotation and epifluorescence...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2020-x
更新日期：2010-11-01 00:00:00
abstract：:Catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including Schistosoma japonicum. S. japonicum tyrosine hydroxylase (SjTH) was amplified by rapid amplification of cDNA ends polymerase chain reaction that shows strong homo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2347-y
更新日期：2011-10-01 00:00:00
abstract：:In schistosomiasis a systemic hyperplasia of the monomacrophagic cell lineage is associated with its mild modifications in myelograms and hemograms. We monitored the in vitro proliferation of myeloid precursors obtained from bone marrow, blood, spleen, and liver. The macrophage colony-forming unit (M-CFU) numbers were...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050467
更新日期：1998-08-01 00:00:00
abstract：:Spermiogenesis and ultrastructure of the spermatozoon of two bothriocephalidean cestodes, Oncodiscus sauridae from the lizardfish Saurida nebulosa Valenciennes, 1850 and Senga sp. from the eel Mastacembelus armatus (Lacepède, 1800), have been studied using transmission electron microscopy. Spermiogenesis included the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2215-1
更新日期：2011-07-01 00:00:00
abstract：:Cyclospora spp. in nonhuman primates are most closely related to Cyclospora cayetanensis, an emerging human pathogen causing outbreaks of cyclosporiasis in North America. Studies thus far indicate the possible existence of host specificity in Cyclospora spp. In this study, 411 fecal specimens from free-range rhesus mo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4367-5
更新日期：2015-05-01 00:00:00
abstract：:This is the first study dealing with the association between HLA alleles and cystic echinococcosis (CE) in Yemeni patients. The present study aimed to detect the association of HLA-DRB(1) alleles and susceptibility or resistance to CE in Yemeni patients by HLA-DRB(1) typing; first by HLA-DRB(1) amplification using PCR...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1868-0
更新日期：2010-07-01 00:00:00
abstract：:This report presents the results of the first faunistic study of hard ticks in Qazvin province of Iran. The primary objective was to determine the species diversity and geographic distribution of hard ticks that parasitize domestic ruminants. Information about the abiotic preferences of these species has been provided...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2501-6
更新日期：2012-01-01 00:00:00
abstract：:Gametocyte proteins are being explored as potential vaccine candidates against Eimeria sp. in chicken since they are the components of the resilient oocyst wall. The aim of this study was to investigate the immunoprophylactic efficacy of recombinant Eimeria tenella gametocyte antigen 22 (EtGam22) in chickens against h...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-06198-2
更新日期：2019-03-01 00:00:00
abstract：:Cystic echinococcosis (CE) is a widespread zoonosis. For treating single echinococcal cysts during the last decades, therapeutic puncture of the cyst, aspiration, injection of a scolicide, and re-aspiration (PAIR) has been established as a minimal-invasive alternative method to surgery. A recent review on the complica...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4275-0
更新日期：2015-02-01 00:00:00
abstract：:Transferrin is a defence protein known to be up-regulated upon infection of parasites/pathogens in Aedes aegypti mosquito. However, no information is available on its up-regulation in Culex quinquefasciatus, the vector of bancroftian filarial parasite. In the present study, enhancement of transferrin in C. quinquefasc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0474-2
更新日期：2007-07-01 00:00:00
abstract：:Pathogen-free rainbow trout (Oncorhynchus mykiss) aged 735 degree days were experimentally exposed to a low dose of infectious Myxobolus cerebralis (20 triactinomyxons fish(-1)). Three time periods were chosen for sampling that included 10 days (d), 67 d, and 5 months (mo) post exposure. Five diagnostic assays were us...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0166-3
更新日期：2006-09-01 00:00:00
abstract：:Rhodnius nasutus, a triatomine species autochthonous in the Brazilian Northeast, is primarily associated with the Copernicia prunifera palm tree (Carnauba). For the first time, the colonization of this triatomine in another tree species is reported. To investigate the existence of an infected triatomine focus located ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0823-1
更新日期：2008-03-01 00:00:00
abstract：:Prior studies have shown that filarial nematodes can effectively metabolize hydrogen peroxide in excess of that generated by activated host cells. However, the mechanisms of H2O2 removal by the filarial parasites are unclear. Herein we report the results of studies carried out on the biochemical activity and on immuno...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050032
更新日期：2000-03-01 00:00:00
abstract：:On a global basis, ticks transmit a greater variety of pathogenic microorganisms, protozoa, rickettsiae, spirochaets, and viruses than any other arthropods and are among the most important vectors of diseases affecting livestock, humans, and companion animals. Ticks and tick-borne diseases (TTBDs) affect 80% of the wo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0684-7
更新日期：2007-09-01 00:00:00
abstract：:Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4327-0
更新日期：2015-04-01 00:00:00
abstract：:Strongyloides stercoralis can cause severe infection both in humans and dogs. Coproparasitological examination has low sensitivity for the diagnosis of this parasite; hence, different diagnostic techniques have been implemented. However, serology and molecular methods have been assessed almost exclusively in humans. I...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5468-0
更新日期：2017-07-01 00:00:00
abstract：:The CSP, EMP2/MESA, MSP2, MSP3, MSP5, RAP1, RAP2, RESA1, SERA1 and SSP2/TRAP genes of Plasmodium falciparum are vaccine candidates. The hepatic phase of the infection is of major interest due to the protection induced by immunization with radiation-attenuated sporozoites. We therefore performed RT-PCR experiments to d...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1061-9
更新日期：2004-04-01 00:00:00
abstract：:The effects of a live Cryptobia salmositica (Kinetoplastida) vaccine on the humoral and cellular immune response, and changes in the peripheral leukocyte populations of Salmo salar were investigated. The vaccine produced detectable parasitemia in the blood which peaked at 5 weeks post-vaccination (w.p.v). Antibodies w...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1270-x
更新日期：2005-03-01 00:00:00
abstract：:The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06796-z
更新日期：2020-09-01 00:00:00
abstract：:Sarcocystis sarcocysts are common in muscles of herbivores but are rare in muscles of carnivores. Here, we report sarcocysts in the muscles of a gray wolf (Canis lupus) from Alaska, USA, for the first time. Sarcocysts extracted from the tongue of the wolf were up to 900 μm long and slender and appeared to have a relat...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5067-5
更新日期：2016-07-01 00:00:00
abstract：:Litomosoides chagasfilhoi is a filariid nematode parasite of the abdominal cavity of the wild rodent Akodon cursor (Winge, 1887), that has been described and used in Brazil as a new model for human filariasis. The fine structure of the intestine of this nematode was analyzed based on observations made by light and tra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0092-9
更新日期：2006-05-01 00:00:00
abstract：:Spermiogenesis of the monozoic cestode Khawia armeniaca (Cholodkovski, 1915), a parasite of Capoeta capoeta sevangi (De Filippi, 1865) (Pisces: Cyprinidae), has been investigated by means of transmission electron microscopy for the first time. The present results show that the type of spermiogenesis in K. armeniaca sh...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0155-6
更新日期：2006-09-01 00:00:00
abstract：:In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phag...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5175-2
更新日期：2016-10-01 00:00:00
abstract：:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Białowieża Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracel...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5549-0
更新日期：2017-09-01 00:00:00
abstract：:We report on serine protease activity in cercarial secretions (CSs) from the bird parasite Trichobilharzia ocellata. Using a colorigenic substrate, the biochemical properties of this enzyme were studied and its activity was compared to the homologous one in CSs from the human parasite Schistosoma mansoni. The specific...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0597-4
更新日期：2002-06-01 00:00:00
abstract：:Tungiasis is endemic in many countries in Latin America, the Caribbean and sub-Saharan Africa, and it is associated with severe morbidity. The pathophysiological and immunological characteristics of the ectoparasitosis are not well understood, and no effective therapy is currently available. The aim of this study was ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0731-4
更新日期：2007-12-01 00:00:00
abstract：:A 1-month-old brown wood owlet (Strix leptogrammica) purchased from a wholesaler and housed as a companion bird by an individual owner in Japan showed severe dehydration and anorexia following a week of vomiting and severe diarrhea. A great number of approximately 5 × 4-μm-sized Cryptosporidium oocysts were found in t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5951-2
更新日期：2018-09-01 00:00:00
abstract：:Representatives of Apicomplexa perform various kinds of movements that are linked to the different stages of their life cycle. Ancestral apicomplexan lineages, including gregarines, represent organisms suitable for research into the evolution and diversification of motility within the group. The vermiform trophozoites...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06381-z
更新日期：2019-09-01 00:00:00
