Abstract：
:Transferrin is a defence protein known to be up-regulated upon infection of parasites/pathogens in Aedes aegypti mosquito. However, no information is available on its up-regulation in Culex quinquefasciatus, the vector of bancroftian filarial parasite. In the present study, enhancement of transferrin in C. quinquefasciatus infected with Wuchereria bancrofti is demonstrated through amplification of the specific mosquito transcript, its sequencing, cloning, and expression. By using two oligonucleotide primers, a 950-bp polymerase chain reaction product was obtained from the first strand cDNA made from RNA of C. quinquefasciatus infected with W. bancrofti. A 707-bp sequence encoding the mature portion of transferrin was confirmed by sequencing the product. This is the first report of transferrin expression in C. quinquefasciatus. The deduced amino acid sequence shared 85% homology with A. aegypti transferrin precursor molecule. Western blot analysis of haemolymph proteins of infected C. quinquefasciatus with antibodies raised against recombinant transferrin protein showed binding to a 66-kDa protein, confirming its identity as transferrin. Hence, this molecule also could be added to the list of immune molecules of C. pipiens group, such as the defensin, gambicin, and cecropin, which are already known.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Paily KP,Kumar BA,Balaraman Kdoi
10.1007/s00436-007-0474-2subject
Has Abstractpub_date
2007-07-01 00:00:00pages
325-30issue
2eissn
0932-0113issn
1432-1955journal_volume
101pub_type
杂志文章abstract：:Mosquitoes transmit dreadful diseases to human beings wherein biological control of these vectors using plant-derived molecules would be an alternative to reduce mosquito population. Aqueous leaf extract and green synthesized silver nanoparticles (Ag NPs) from Pedilanthus tithymaloides (L.) Poit. were investigated for...
journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1990-01-01 00:00:00
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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更新日期：2009-09-01 00:00:00
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更新日期：2020-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00436-012-2878-x
更新日期：2012-08-01 00:00:00
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journal_title：Parasitology research
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doi：10.1007/s00436-005-1436-1
更新日期：2005-10-01 00:00:00
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更新日期：2009-09-01 00:00:00
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journal_title：Parasitology research
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doi：10.1007/s00436-014-3926-5
更新日期：2014-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00936174
更新日期：1992-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 历史文章,杂志文章,评审
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更新日期：2011-01-01 00:00:00
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journal_title：Parasitology research
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更新日期：2014-07-01 00:00:00
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doi：10.1007/s00436-006-0423-5
更新日期：2007-05-01 00:00:00
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journal_title：Parasitology research
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更新日期：2005-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2002-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2003-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2015-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06988-7
更新日期：2021-01-08 00:00:00
abstract：:The morphology, adult development and genetic characteristics of Echinococcus granulosus isolated from pigs in Poland were examined and compared with those of other recognised strains of E. granulosus. The isolates were characterised by their distinct morphology, rapid maturation and unique DNA hybridisation profiles....
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931216
更新日期：1993-01-01 00:00:00
