Abstract：
:Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Ribeiro Vda S,Nunes Dda S,Gonzaga HT,da Cunha-Junior JP,Costa-Cruz JMdoi
10.1007/s00436-014-3908-7subject
Has Abstractpub_date
2014-07-01 00:00:00pages
2569-75issue
7eissn
0932-0113issn
1432-1955journal_volume
113pub_type
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