Abstract：
:In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used fast protein liquid chromatography (FPLC)-fractionated extracts of F. hepatica excretory/secretory antigens (E/S Ags) for serodiagnosis of human and sheep fasciolosis. To develop an improved diagnostic method, we fractionated F. hepatica E/S Ags by anion exchange chromatography on a Sepharose CL-6B column and then tested the serodiagnostic values of the fractions. We used sera from F. hepatica-infected human and sheep as positive controls. Sera from patients with hydatidosis and strongyloidiasis were used for cross-reactivity studies. Enzyme-linked immunosorbent assays (ELISA) of the second FPLC peak, containing 20, 25, and 70 kDa proteins, discriminated between F. hepatica-infected and uninfected human and sheep samples. Fractionation of F. hepatica E/S Ags by FPLC is a fast and reproducible way of obtaining antigens useful for serodiagnosis of human and sheep fasciolosis with acceptable sensitivity and specificity. Graphical abstract ᅟ.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Mokhtarian K,Akhlaghi L,Meamar AR,Razmjou E,Manouchehri Naeini K,Gholami S,Najafi Samei M,Falak Rdoi
10.1007/s00436-016-5049-7subject
Has Abstractpub_date
2016-08-01 00:00:00pages
2957-65issue
8eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5049-7journal_volume
115pub_type
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