Abstract:
:Tick proteins have been shown to be useful for the development of vaccines which reduce tick infestations. Potential tick protective antigens have been identified and characterized, in part, by use of RNA interference (RNAi). RNAi allows for analysis of gene function by characterizing the impact of loss of gene expression on tick physiology. Herein, we used RNAi in Rhipicephalus sanguineus to evaluate gene functions of two tick protective antigens, 4D8 and Rs86, the homologue of Bm86, on tick infestation, feeding and oviposition. Silencing of 4D8 alone resulted in decreased tick attachment, survival, feeding and oviposition. Although the effect of Rs86 RNAi was less pronounced, silencing of this gene also reduced tick weight and oviposition. Most notably, simultaneous silencing of 4D8 and Rs86 by RNAi resulted in a synergistic effect in which tick survival, attachment, feeding, weight and oviposition were profoundly reduced. Microscopic evaluation of tick tissues revealed that guts from dual injected ticks were distended with epithelial cells sparsely distributed along the basement membrane. These results demonstrated the synergistic effect of the silencing expression of two tick protective genes. Inclusion of multiple tick protective antigens may, therefore, enhance the efficacy of tick vaccines.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
de la Fuente J,Almazán C,Naranjo V,Blouin EF,Kocan KMdoi
10.1007/s00436-006-0132-0subject
Has Abstractpub_date
2006-07-01 00:00:00pages
108-13issue
2eissn
0932-0113issn
1432-1955journal_volume
99pub_type
杂志文章abstract::The pathogenicity of 19 hypocrealean entomopathogenic fungi from seven different genera in adult Aedes aegypti was tested. All fungi proved to be pathogenic, and Isaria fumosorosea, Lecanicillium muscarium, Lecanicillium psalliotae, Metarhizium anisopliae, Metarhizium lepidiotae, Metarhizium majus, Metarhizium frigidu...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1991-y
更新日期:2010-10-01 00:00:00
abstract::The present report describes a method for establishment of colonies of Blastocystis hominis from single cells in soft agar. The percentage of colony-forming efficiency (% CFE = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. Fiv...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050194
更新日期:1996-01-01 00:00:00
abstract::The apparent scarcity or absence of blood parasites in some avian groups, such as seabirds, has been related to intrinsic and extrinsic factors including host immunological capacity, host-parasite assemblage, and ecological parameters, but also to reduced sensitivity of some methods to detect low parasite prevalence/i...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-017-5676-7
更新日期:2018-01-01 00:00:00
abstract::A molecular phylogenetic hypothesis is presented for the genus Trichuris based on sequence data from mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob). The taxa consisted of nine populations of whipworm from five species of Sigmodontinae rodents from Argentina. Bayesian Inference, Maximum Parsimony, a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5045-y
更新日期:2016-08-01 00:00:00
abstract::The outbreak of bluetongue disease in Central Europe necessitates new approaches in the identification of vectors to follow-up changes of populations of species and not of complexes. Since females of species of the complex of Culicoides obsoletus are difficult to be identified according to morphological criteria, we a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1412-2
更新日期:2009-08-01 00:00:00
abstract::In 1978, the nature of the hypnozoite was discussed in an article that appeared in a relatively obscure journal, which is also where the term was adopted for Plasmodium (a little-known fact). As a result, that commentary on the use of the word "hypnozoite" has been almost completely overlooked. Although the publicatio...
journal_title:Parasitology research
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00436-010-2072-y
更新日期:2011-01-01 00:00:00
abstract::Antroponotic cutaneous leishmaniasis (ACL) is an endemic disease and one of the major health problems in Sanliurfa province located in the southeastern region of Turkey. Leishmania tropica is confirmed as the causative agent of ACL in this region. In Sanliurfa city alone, the recorded total cases of ACL were 6,817 bet...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1013-5
更新日期:2008-08-01 00:00:00
abstract::Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the spe...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3325-3
更新日期:2013-04-01 00:00:00
abstract::The leaf extract of Acalypha alnifolia with different solvents - hexane, chloroform, ethyl acetate, acetone and methanol - were tested for larvicidal activity against three important mosquitoes such as malarial vector, Anopheles stephensi, dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2525-y
更新日期:2012-02-01 00:00:00
abstract::Myiasis refers to the infestation of living humans and vertebrates with fly larvae that feed on necrotic or vital tissue of the host. Since the invasion of new fly species in Europe is currently being observed, which live obligatorily parasitically or are close relatives of such species, the aim of this study is to ob...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6145-7
更新日期:2019-02-01 00:00:00
abstract::The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0165-4
更新日期:2006-07-01 00:00:00
abstract::Little information is available on the occurrence of endoparasites in pet guinea pigs (Cavia porcellus). The purpose of this study was to evaluate the prevalence of intestinal parasites in cavies kept as pets in southern Italy. Fresh fecal samples were randomly collected from 60 guinea pigs housed in pet shops or priv...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4289-7
更新日期:2015-03-01 00:00:00
abstract::In the search of alternatives for the control of mosquitoes of medical importance, we evaluated the larvicidal activity of micronized suspensions of calcium hydroxide [Ca(OH)₂] against Aedes aegypti and Culex quinquefasciatus. Tests conducted under laboratory conditions determined a LC50 = 0.027% (LC90 = 0.096%) for A...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2593-z
更新日期:2012-03-01 00:00:00
abstract::Blastocystis hominis is a common intestinal parasite observed in fecal examination. On the other hand, the transmission of this parasite is certainly unknown. The transmission of B. hominis can be realized by animal contact and the contamination by water and food with excreted cysts from the reservoir hosts. B. homini...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1937-4
更新日期:2010-09-01 00:00:00
abstract::The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease b...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050435
更新日期:1998-06-01 00:00:00
abstract::Spermiogenesis of the monozoic cestode Khawia armeniaca (Cholodkovski, 1915), a parasite of Capoeta capoeta sevangi (De Filippi, 1865) (Pisces: Cyprinidae), has been investigated by means of transmission electron microscopy for the first time. The present results show that the type of spermiogenesis in K. armeniaca sh...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0155-6
更新日期:2006-09-01 00:00:00
abstract::In Romania, cystic echinococcosis is endemic and affects, besides humans, various animal species including sheep, cattle, and swine. As yet, no molecular-genetic typing has been carried out to clearly identify the putative strains being transmitted there. Parasite samples (protoscoleces or germinal layers) were collec...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-005-0015-9
更新日期:2006-01-01 00:00:00
abstract::Amphibian populations are declining around the world, and the main reasons are the environmental changes and pathogens. However, there are few studies addressing the interaction and impact of the different pathogens that affect amphibians, such as hemoparasites. These parasites had been described as common in some amp...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06689-1
更新日期:2020-06-01 00:00:00
abstract::The effect of incubation of parasites (epimastigote and trypomastigote forms of Trypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process of N-glycosylation of p...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00930829
更新日期:1989-01-01 00:00:00
abstract::Global pollinator decline has recently been discussed in the context of honey and bumble bee infections from various pathogens including viruses, bacteria, microsporidia and mites. The microsporidian pathogens Nosema apis, Nosema ceranae and Nosema bombi may in fact be major candidates contributing to this decline. Di...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2640-9
更新日期:2012-04-01 00:00:00
abstract::Several murine monoclonal antibodies were raised against purified soluble Plasmodium falciparum antigens from the asexual blood stage. The monoclonal antibodies were purified from ascites by preparative agarose gel electrophoresis and tested for inhibitory activities against P. falciparum in vitro cultures. One monocl...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00535326
更新日期:1987-01-01 00:00:00
abstract::Ticks are hematophagous ectoparasites which can transmit several diseases to the host during their feeding process. When ticks mechanically damage the tissue, they eventually induce inflammatory responses on the skin spot where they are fixed. One of the alternatives to control these ectoparasites is the use of chemic...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3422-3
更新日期:2013-07-01 00:00:00
abstract::The migratory response of peripheral blood granulocytes and monocytes from the European eel Anguilla anguilla (L.) to infective larvae of the swimbladder nematode Anguillicola crassus Kuwahara, Niimi and Hagaki, 1974 was examined by means of light microscopical histology and with an in vitro assay using a modified Boy...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-0910-y
更新日期:2008-05-01 00:00:00
abstract::The CSP, EMP2/MESA, MSP2, MSP3, MSP5, RAP1, RAP2, RESA1, SERA1 and SSP2/TRAP genes of Plasmodium falciparum are vaccine candidates. The hepatic phase of the infection is of major interest due to the protection induced by immunization with radiation-attenuated sporozoites. We therefore performed RT-PCR experiments to d...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1061-9
更新日期:2004-04-01 00:00:00
abstract::This study aimed to identify tick species and to determine their relationship with the Theileria parva seroprevalence in cattle raised under an extensive farming system in North Kivu Province, Democratic Republic of Congo in two agro-ecological zones namely medium (1,000-1,850 m) and high (>1,850 m) altitude. Among th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-3200-7
更新日期:2013-02-01 00:00:00
abstract::Dendrimers are repetitively branched molecules with a broad spectrum of applications, mainly for their antimicrobial properties and as nanocarriers for other molecules. Recently, our research group have synthesized and studied their activity against Acanthamoeba sp., causative agent of a severe ocular disease in human...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4205-1
更新日期:2015-02-01 00:00:00
abstract::This work investigated the anti-amoebic activity of two samarium (Sm) complexes, the acyclic complex [bis(picrato)(pentaethylene glycol)samarium(III)] picrate-referred to as [Sm(Pic)2(EO5)](Pic)-and the cyclic complex [bis(picrato)(18-crown-6)samarium(III)] picrate-referred to as [Sm(Pic)2(18C6)](Pic). Both Sm complex...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5814-x
更新日期:2018-05-01 00:00:00
abstract::In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5049-7
更新日期:2016-08-01 00:00:00
abstract::A low molecular weight (LMW) antigen recognized by a murine monoclonal antibody (C(3)4F(1)) was localized within endogenous stages of Eimeria tenella (USDA strain 80). Using indirect fluorescent antibody assay and immunoelectron microscopy, the LMW antigen was found in: sporozoites, first, second and third generation ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050693
更新日期:2000-06-01 00:00:00
abstract::P-Glycoprotein (P-GP) is a transport protein that participates in the mechanism of active secretion of different molecules from the bloodstream to the gastrointestinal tract. The aim of the current work was to evaluate the effect of verapamil, a P-GP substrate, on the pharmacokinetic behaviour of the anthelmintics ive...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1022-3
更新日期:2004-01-01 00:00:00