Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate.

Abstract:

:The present report describes a method for establishment of colonies of Blastocystis hominis from single cells in soft agar. The percentage of colony-forming efficiency (% CFE = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. Five human Blastocystis isolates chosen for this study showed no apparent variation in colonial morphology. Isolated colonies were also successfully grown in liquid medium, providing a means of obtaining large numbers of B. hominis cells that had arisen from a single clone.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Tan SW,Singh M,Thong KT,Ho LC,Moe KT,Chen XQ,Ng GC,Yap EH

doi

10.1007/s004360050194

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

737-9

issue

8

eissn

0932-0113

issn

1432-1955

journal_volume

82

pub_type

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