Abstract：
:The effect of incubation of parasites (epimastigote and trypomastigote forms of Trypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process of N-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote forms, respectively. Treatment of the macrophages with TM increased by about 65% and reduced by about 45% the ingestion of epimastigote and trypomastigote forms, respectively. Treatment of the parasites or the macrophages with monensin, a drug which interferes with the sorting of membrane proteins, significantly reduced the ingestion of epimastigote and trypomastigote forms by the macrophages. The effects of both drugs were reversible. Treatment of the macrophages with trypsin followed by their incubation in fresh medium containing tunicamycin or monensin was used to analyse further the effects of these two drugs on the macrophages. The effects of the drugs on macrophages and parasites were controlled by electron microscopy. The results obtained suggest that the N-glycosylated proteins exposed on the surface of both cells are involved in the process of parasite-macrophage interaction and that interference in the process of sorting of membrane components alters the interaction.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Souto-Padrón T,de Souza Wdoi
10.1007/BF00930829subject
Has Abstractpub_date
1989-01-01 00:00:00pages
98-106issue
2eissn
0932-0113issn
1432-1955journal_volume
76pub_type
杂志文章abstract：:Necrophagous Sarcophagidae are among the insects most frequently reported from human corpses. The broad forensic application of flesh flies is restricted by the lack of reliable tools for species identification of larval stages and mass breeding of collected flesh fly larvae to the adult stage, and more recently DNA-b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4421-3
更新日期：2015-06-01 00:00:00
abstract：:The overall risk for toxoplasmosis in meat produced in Colombia is unknown. We analyzed by PCR assay meat samples for human consumption in two types of plants in Colombia: 120 samples from class I plants (60 samples from chicken, 30 from swine and 30 from beef) and 60 from class II plants (30 samples from beef and 30 ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4790-7
更新日期：2016-02-01 00:00:00
abstract：:The use of pyrethroids to control the human head louse, Pediculus humanus capitis De Geer (Anoplura: Pediculidae), has suffered considerable loss of efficacy due to the evolution of resistance. Thus, the development of efficiently insecticide delivery systems is imperative for the control of head lice. We studied the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5510-2
更新日期：2017-08-01 00:00:00
abstract：:The following effects of Lactobacillus casei in NIH mice were evaluated: the establishment of Trichinella spiralis adult worms in the intestine (AWI), larvae per gram of muscle tissue (LPG), levels of IgG and IgA, and levels of IL-4 and IFN-γ. One hundred and eight mice were allocated at random into 18 groups of six m...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2432-2
更新日期：2011-12-01 00:00:00
abstract：:To date classification and differentiation of Alaria spp. are based largely on external characteristics and comparative morphology of adult flukes. The accurate differentiation between various Alaria spp. mesocercariae is indeed difficult because there are only few data on morphological and morphometrical features of ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2240-0
更新日期：2011-05-01 00:00:00
abstract：:A novel cDNA encoding a lysophospholipase (SmLPLH) homologue has been cloned from the human parasite, Schistosoma mansoni. The predicted primary structure of SmLPLH shares high sequence homology with mammalian lysophospholipases (62%) and also contains aconsensus signature peptide (GXSXG), which is present in all lipa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050497
更新日期：1998-10-01 00:00:00
abstract：:The development of the fourth larval stage (L4) of Anisakis physeteris was studied using scanning electron microscopy (SEM), comparing it with third larval stage (L3) recently obtained from the host fish, blue whiting (Micromesistius poutassou), from the western Mediterranean Sea (east coast of Spain, zone FAO 37.1.1)...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5896-5
更新日期：2018-07-01 00:00:00
abstract：:Changes in the hepatocytes of male hamsters (Mesocricetus auratus) and jirds (Meriones unguiculatus) at 220 days after experimental infection with the liver fluke Opisthorchis viverrini were studied by light and electron microscopy. The hepatocytes of the control group were characterized by an intracellular compartmen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931823
更新日期：1993-01-01 00:00:00
abstract：:Considering the snake venoms' pharmacological properties and chemotherapeutic potential as well as the need for new alternatives for Giardia infection treatment, the present study was carried out aiming to evaluate the in vitro effects of crude Crotalus durissus terrificus and Bothrops jararaca venoms on the growth an...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0037-3
更新日期：2006-03-01 00:00:00
abstract：:The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5539-2
更新日期：2017-08-01 00:00:00
abstract：:The tegument proteins of schistosomes are regarded as potential vaccine candidates and drug targets to control schistosomiasis. Nucleotide pyrophosphatase/phosphodiesterase-5 (NPP-5), which belongs to a multigene family of nucleotide pyrophosphatase/phosphodiesterases (NPPs), is important in the hydrolysis of pyrophos...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2552-8
更新日期：2012-02-01 00:00:00
abstract：:Eimeria tenella-specific antibodies were examined for the cross-reactivity on the sporozoites and merozoites of E. tenella, Eimeria maxima, Eimeria acervulina and Eimeria brunetti in an indirect fluorescence antibody test. Two of nine antibodies showed cross-reactivity with sporozoites of E. maxima, E. acervulina and ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2171-9
更新日期：2011-03-01 00:00:00
abstract：:Microsporidia is a group of spore-forming microorganisms with zoonotic potential. This study aimed to compare intestinal microsporidia infections in cat owners and non-pet owners. In total, 210 fecal samples were collected from indoor cats, cat owners, and non-pet owners. DNA extraction was performed and the small sub...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06690-8
更新日期：2020-06-01 00:00:00
abstract：:We have identified the 2-Cys peroxiredoxin (Prx) from a bovine Babesia parasite, B. bovis. Prx is a recently described family of antioxidant enzymes that are highly conserved in eukaryotes and prokaryotes. B. bovis 2-Cys Prx (BbTPx-1) contained two conserved cysteine residues that corresponded to Cys47 and Cys170 of t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1587-6
更新日期：2009-10-01 00:00:00
abstract：:The beta-tubulin coding sequences were investigated for the Chinese liver fluke, Clonorchis sinensis. A cDNA library of adult C. sinensis was screened with cDNA probes synthesized by polymerase chain reaction using degenerate primers, and two beta-tubulin cDNAs, CsTB1 and CsTB3, were subsequently cloned. The CsTB1 and...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1512-z
更新日期：2009-10-01 00:00:00
abstract：:In temperate regions, some avian haemosporidian parasites have evolved seasonal transmission strategies, with chronic infections relapsing during spring and transmission peaking during the hosts' breeding season. Because lineages with seasonal transmission strategies are unlikely to produce gametocytes in winter, we p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06646-y
更新日期：2020-05-01 00:00:00
abstract：:Nosema ceranae is a ubiquitous microsporidian pathogen infecting the midgut of honey bees. The infection causes bee nosemosis, a disease associated with malnutrition, dysentery, and lethargic behavior, and results in considerable economic losses in apiculture. The use of a rapid, sensitive, and inexpensive DNA-based m...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06915-w
更新日期：2020-12-01 00:00:00
abstract：:Equine ocular setariasis arising mainly from ectopic infestation of Setaria digitata is a common vision impairing ophthalmic disease in India, and the identification of this filarial nematode is based solely on morphology. However, morphological characters alone are inadequate to detect and differentiate S. digitata f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06587-1
更新日期：2020-02-01 00:00:00
abstract：:The present paper reports the ability of egg-propagated gametocyte vaccine to induce protective immunity against Eimeria tenella infections in offspring chicks. For this purpose, breeding hens were immunized with adjuvanted gametocyte vaccine orally, being the best vaccine in our previous studies. Eggs collected 2 wee...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0382-x
更新日期：2007-04-01 00:00:00
abstract：:The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C. parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1250-7
更新日期：2009-03-01 00:00:00
abstract：:The authors previously reported that an extract from Zingiber officinale, traditionally eaten along with raw fish and used in traditional Chinese medicine, effectively destroyed Anisakis larvae in vitro. In this study, we analyzed the effective components of ginger rhizomes. Methanol extracts were fractionated after f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931082
更新日期：1990-01-01 00:00:00
abstract：:Human toxocariasis may be acquired by eating raw chicken liver. However, there are no reports on the prevalence of natural infection of chickens with Toxocara. The aim of this study was to evaluate the presence of anti-Toxocara antibodies as indicators of natural infection with Toxocara, in free-range chickens from Es...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4669-7
更新日期：2015-11-01 00:00:00
abstract：:Haemoproteus spp. are cosmopolitan vector-born haemosporidian parasites, some species of which cause diseases in non-adapted birds. Recent polymerase chain reaction (PCR)-based studies have detected mitochondrial cytochrome b gene lineages of these Haemoproteus parasites in blood-sucking mosquitoes and speculated abou...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3375-6
更新日期：2013-06-01 00:00:00
abstract：:The prevalence and abundance of infections with haemoparasites were studied over a 4-year period in Microtus arvalis (common vole, n =321) sampled from fallow grassland sites in north-eastern Poland. Total species richness was five (prevalence= Haemobartonella sp. 63.9%, Bartonella spp. 27.7%, Babesia microti 9.0%, Tr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1040-1
更新日期：2004-03-01 00:00:00
abstract：:The present study uses scanning and transmission electron microscopical observations to examine the haptoral features of the hexabothriid polyopisthocotylean monogenean Rajonchocotyle emarginata from the gills of the elasmobranch Amblyraja radiata. The haptor possesses six equal, pedunculate suckers, each armed with a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4973-x
更新日期：2016-06-01 00:00:00
abstract：:Molecular karyotypes that revealed size polymorphisms between homologous forms of chromosomes 1, 2, 3 or 4 in three strains of the coccidial parasite Eimeria tenella were derived by pulsed field gel electrophoresis (PFGE) using brands of agaroses marketed either specifically for PFGE or for purposes such as protein el...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0775-4
更新日期：2003-03-01 00:00:00
abstract：:In murine Taenia crassiceps cysticercosis, females sustain larger intensities of infection than males. However, during chronic infection, this difference disappears and males show a feminization process. To further study the role of two cytokines, interleukin-6 (IL-6) and macrophage-migration inhibitory factor (MIF), ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0674-8
更新日期：2002-10-01 00:00:00
abstract：:In the present study, we sequenced and analyzed the mitochondrial (mt) genome of Gastrothylax crumenifer and compared it with other selected trematodes. The full mt genome of G. crumenifer was amplified, sequenced, assembled, analyzed and then subjected to phylogenetic analysis. The complete mt genome of G. crumenifer...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5019-0
更新日期：2016-06-01 00:00:00
abstract：:Cystic echinococcosis (CE) is a widespread zoonosis. For treating single echinococcal cysts during the last decades, therapeutic puncture of the cyst, aspiration, injection of a scolicide, and re-aspiration (PAIR) has been established as a minimal-invasive alternative method to surgery. A recent review on the complica...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4275-0
更新日期：2015-02-01 00:00:00
abstract：:Two French populations of Galba truncatula were subjected to experimental infections with Egyptian and French isolates of Fasciola sp. miracidia, originating from cattle and sheep, to compare characteristics of snail infections in allopatric and sympatric groups. All sampled Egyptian isolates were identified as Fascio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3421-4
更新日期：2013-07-01 00:00:00
