Abstract：
:To date classification and differentiation of Alaria spp. are based largely on external characteristics and comparative morphology of adult flukes. The accurate differentiation between various Alaria spp. mesocercariae is indeed difficult because there are only few data on morphological and morphometrical features of the parasite's developmental stages. We established a conventional polymerase chain reaction (PCR) for a molecular-based diagnosis of Alaria alata mesocercariae that can aid in their identification. Twenty Alaria spp. mesocercariae specimens were collected from three different wild boars originating from different areas of eastern Germany. DNA from the prepared isolates was extracted, and a primer pair was selected to amplify a 303-bp region of the A. alata genome. The DNA preparations extracted from the field samples as well as A. alata positive controls were successfully amplified and yielded a single sharp band of the expected size. In all samples, molecular identification was consistent with morphological identification. With our new PCR assay, we present the first approach for identification and characterization of A. alata mesocercariae specimens using molecular methods. This practicable and reproducible protocol can be used for both diagnostic and epidemiological purposes.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Riehn K,Hamedy A,Alter T,Lücker Edoi
10.1007/s00436-010-2240-0subject
Has Abstractpub_date
2011-05-01 00:00:00pages
1327-32issue
5eissn
0932-0113issn
1432-1955journal_volume
108pub_type
杂志文章abstract：:Ticks and tick-borne diseases (TBDs) continue to pose an insidious and ever-present threat to livestock and livelihoods across the globe. Two of the most significant TBDs of cattle in Africa are heartwater and babesioisis, caused by Ehrlichia ruminantium and Babesia bigemina respectively. Both pathogens are endemic in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06204-1
更新日期：2019-03-01 00:00:00
abstract：:The effects of a live Cryptobia salmositica (Kinetoplastida) vaccine on the humoral and cellular immune response, and changes in the peripheral leukocyte populations of Salmo salar were investigated. The vaccine produced detectable parasitemia in the blood which peaked at 5 weeks post-vaccination (w.p.v). Antibodies w...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1270-x
更新日期：2005-03-01 00:00:00
abstract：:Spermiogenesis and spermatozoon ultrastructure of the caryophyllidean cestode Breviscolex orientalis Kulakovskaya, 1962, first member of the family Capingentidae studied, a parasite of cyprinid fish Abbottina rivularis, are described using transmission electron microscopy. Spermiogenesis in B. orientalis follows the T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2144-z
更新日期：2011-04-01 00:00:00
abstract：:Snake venom can affect the growth of Trypanosoma cruzi and Leishmania spp. As new classes of therapeutic drugs against protozoan parasites could be derived from snake venom, alterations in the ultrastructure and growth of the epimastigotes, trypomastigotes and amastigotes of T. cruzi, as well as the promastigotes of L...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0626-3
更新日期：2002-07-01 00:00:00
abstract：:Ticks can significantly affect the health and fitness of the host. Seasonal population dynamics of ticks play a vital role in disease transmission and the shaping of life-history traits of both tick and host. In this study, we examine the seasonal population dynamics of Ixodes hirsti in South Australia. For 2 years, w...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1071-8
更新日期：2008-09-01 00:00:00
abstract：:Knowledge of species and genotypes on Cryptosporidium involved in human transmission is fundamental in studying the epidemiology of cryptosporidiosis. This is the first study in molecular epidemiology of cryptosporidiosis that has been made in Chile. Cryptosporidium parvum species and genotypes involved in human and b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1391-x
更新日期：2005-08-01 00:00:00
abstract：:Sarcocystis neurona is the parasite associated with equine protozoal myeloencephalitis (EPM). Recently, cats (Felis domesticus) have been implicated as a potential intermediate host in the life cycle of S. neurona. This study was initiated to determine whether cats have antibodies that react to S. neurona antigens sim...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100503
更新日期：2002-01-01 00:00:00
abstract：:Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocystis ratti n. sp. Und...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06393-9
更新日期：2019-09-01 00:00:00
abstract：:Interferon-tau (IFN-tau) treatment of Trichinella spiralis-infected BALB/c mice was investigated. The therapeutic regimen consisted of daily intraperitoneal injection of 10(4) U murine IFN-tau for 7 days, starting at 2 weeks post-infection. Striated muscle samples (diaphragm, thigh) were collected at 4, 8 and 12 weeks...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931641
更新日期：1991-01-01 00:00:00
abstract：:Tetracapsuloides bryosalmonae (Myxozoa) is the causative agent of proliferative kidney disease in various species of salmonids which are found in Europe and North America. Less information about the interactions of T. bryosalmonae proteins with salmonid proteins during parasite development is known. In this study, ant...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4357-7
更新日期：2015-05-01 00:00:00
abstract：:Global pollinator decline has recently been discussed in the context of honey and bumble bee infections from various pathogens including viruses, bacteria, microsporidia and mites. The microsporidian pathogens Nosema apis, Nosema ceranae and Nosema bombi may in fact be major candidates contributing to this decline. Di...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2640-9
更新日期：2012-04-01 00:00:00
abstract：:Maturation of trematode larval stages is expected to be temporally and spatially adapted to maximise the encounter with the adequate downstream host, i.e. the host, which will be infected by this parasite stage. Since studies on intramolluscan parasite maturation are scarce but important in the context of parasite tra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4774-7
更新日期：2016-02-01 00:00:00
abstract：:Spermiogenesis of the pseudophyllidean tapeworm Eubothrium crassum has been described by the aid of transmission electron microscopy for the first time. Initially, early spermatids form a distal cytoplasmic protrusion, a differentiation zone containing a small electron-dense, apically oriented region. Out of this regi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100392
更新日期：2001-08-01 00:00:00
abstract：:Acanthamoeba are found in the environment, particularly in water, all over the world. The genus is currently classified into 20 different genotypes, T1-T20. In this study, 63 natural water samples from 11 provinces in northeast Thailand were collected and cultured on non-nutrient agar plates. Positive samples by cultu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4911-y
更新日期：2016-04-01 00:00:00
abstract：:Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4327-0
更新日期：2015-04-01 00:00:00
abstract：:Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5343-4
更新日期：2017-02-01 00:00:00
abstract：:Pharmacokinetics and anthelmintic activity of topical eprinomectin in goats prevented from physical contact to others and self-grooming were studied. Sixteen approximately 7 months old male castrated German White Noble goats harbouring induced infections of gastrointestinal nematode parasites were included in the stud...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4072-9
更新日期：2014-11-01 00:00:00
abstract：:Exsheathment and midgut invasion of nocturnally subperiodic Brugia malayi microfilariae were analyzed using light and scanning electron microscopy in a refractory vector, Aedes aegypti (Thailand strain). Results showed that exsheathed microfilariae represented only approximately 1% of the total microfilaria midguts di...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4086-3
更新日期：2014-11-01 00:00:00
abstract：:WD40-repeat proteins have four to eight repeating units flanked by Gly-His (GH) and Trp-Asp (WD) at both termini and folds into a beta-propeller. A polypeptide deduced from a Clonorchis sinensis cDNA clone analyzed to have seven WD40-repeats and predicted to form a beta-propeller (CsWD1). The CsWD1 protein was express...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0721-6
更新日期：2007-12-01 00:00:00
abstract：:We have identified the 2-Cys peroxiredoxin (Prx) from a bovine Babesia parasite, B. bovis. Prx is a recently described family of antioxidant enzymes that are highly conserved in eukaryotes and prokaryotes. B. bovis 2-Cys Prx (BbTPx-1) contained two conserved cysteine residues that corresponded to Cys47 and Cys170 of t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1587-6
更新日期：2009-10-01 00:00:00
abstract：:Diagnostic parameters for visceral leishmaniasis (VL), a potentially fatal parasitic disease caused by Leishmania donovani, have been redefined in the last decade with the development of serological and molecular tests, though a definitive diagnosis still banks on the century-old parasitological methods in many areas....
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-011-2680-1
更新日期：2012-03-01 00:00:00
abstract：:Little information is available on the occurrence of endoparasites in pet guinea pigs (Cavia porcellus). The purpose of this study was to evaluate the prevalence of intestinal parasites in cavies kept as pets in southern Italy. Fresh fecal samples were randomly collected from 60 guinea pigs housed in pet shops or priv...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4289-7
更新日期：2015-03-01 00:00:00
abstract：:The effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro application was done...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5056-8
更新日期：2016-08-01 00:00:00
abstract：:In 2001, an international surveillance initiative was established, utilising a validated larval development inhibition assay to track the susceptibility of cat flea isolates to imidacloprid. In 2009, an Australian node was incorporated into the programme, joining laboratories in the United States and Europe. Field iso...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3280-z
更新日期：2013-08-01 00:00:00
abstract：:The efficacy of emodepside plus toltrazuril (Procox® oral suspension for dogs) against different species of gastrointestinal nematodes (Toxocara canis, Ancylostoma caninum, Uncinaria stenocephala) was evaluated in nine randomised,blinded and placebo-controlled laboratory studies in naturally or experimentally infected...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-011-2397-1
更新日期：2011-08-01 00:00:00
abstract：:This paper follows the study from 2013 focused on the molecular screening of mosquitoes as vectors of Dirofilaria spp. which provided the information on Aedes vexans as a potential vector of Dirofilaria repens in Slovakia. Current entomological and molecular research indicates that Ae. vexans can participate also in t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4692-8
更新日期：2015-12-01 00:00:00
abstract：:Ultrastructural characters of the spermiogenesis and mature spermatozoon of Notopentorchis sp. (Cestoda, Cyclophyllidea, Paruterinidae), a parasite from Apus affinis (Aves, Apodiformes, Apodidae) from Gabon, are described by means of transmission electron microscopy. Cytochemical analysis for detection of glycogen was...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2809-2
更新日期：2012-07-01 00:00:00
abstract：:Blastocystis is a zoonotic protozoan parasite frequently identified in the intestinal tract of humans and a vast variety of animals, worldwide. Here, we assessed the prevalence of Blastocystis and its subtypes in stool samples of raccoons. Stool samples from 30 raccoons were collected. Total DNA was extracted, and the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06770-9
更新日期：2020-08-01 00:00:00
abstract：:Investigations by scanning and transmission electron microscopy and freeze-etching revealed that eyes of adult Rhipicephalus evertsi mimeticus consist of a lens and photoreceptor cells, which are separated by an acellular layer and the hypodermis. The lens contains numerous pore channels, which open beneath the epicut...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00933552
更新日期：1990-01-01 00:00:00
abstract：:Ivermectin at single doses of 0.2-1.0 mg/kg body weight reduced the microfilarial counts of subperiodic Brugia malayi in Presbytis cristata by 59.9%-89.6% of initial counts, 4 weeks after treatment. Adult filaricidal activity was poor, live adult worms being recovered from all animals at autopsy. There was no serious ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00539462
更新日期：1988-01-01 00:00:00
