Abstract:
:The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C. parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle from some farms around Osaki area, Miyagi prefecture, Japan, was examined on a field visit during August, 2007, by polymerase chain reaction techniques for detection, genotyping, and subtyping. Cryptosporidium oocysts were detected in the feces of five out of 50 animals. Of the five Cryptosporidium-positive specimens available for molecular analysis, C. parvum was identified in three specimens, Cryptosporidium deer-like genotype in one, and Cryptosporidium andersoni in one specimen. Amplification of Cpgp60 from C. andersoni and Cryptosporidium deer-like genotype samples revealed that these samples have light concurrent C. parvum infection. Sequence analysis of the 60-kDa glycoprotein gene indicated that all C. parvum samples are IIa subtype. Detection of Cryptosporidium deer-like genotype is geographically unique in Japan. The genetic diversity of Cryptosporidium in dairy cattle in Japan may be much greater than that reported before.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Amer S,Honma H,Ikarashi M,Oishi R,Endo M,Otawa K,Nakai Ydoi
10.1007/s00436-008-1250-7subject
Has Abstractpub_date
2009-03-01 00:00:00pages
745-52issue
4eissn
0932-0113issn
1432-1955journal_volume
104pub_type
杂志文章abstract::Distinct sequences of Giardia duodenalis assemblages raised the hypothesis that certain assemblages may contribute to its clinical outcome. However, sequences analysis is time consuming, expensive, and needs many manual operations. Nested PCR targeting intergenic spacer (IGS) region was applied successfully to genotyp...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5347-0
更新日期:2017-02-01 00:00:00
abstract::Potent compounds do not necessarily make the best drugs in the market. Consequently, with the aim to describe tools that may be fundamental for refining the screening of candidates for animal and preclinical studies and further development, molecules of different structural classes synthesized within the frame of a br...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4210-4
更新日期:2015-02-01 00:00:00
abstract::According to the genetic characterization by the analysis of the miniexon gene, strains of Trypanosoma cruzi can be classified into six discrete typing units (DTUs), and the DTU 1 into four distinct genotypes associated with different life cycles. While Chagas disease is endemic in Costa Rica, T. cruzi isolates from t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-3000-0
更新日期:2012-10-01 00:00:00
abstract::The purpose of this study was to evaluate the practicality of using real-time PCR for quantifying feces-derived trichostrongyle eggs. Haemonchus contortus eggs were used to evaluate fecal contaminants, time after egg embryonation, and the presence of competing and non-competing DNAs as factors that might interfere wit...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0428-0
更新日期:2007-06-01 00:00:00
abstract::The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence al...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-005-0036-4
更新日期:2006-04-01 00:00:00
abstract::Under natural conditions, eukaryote cells may contain bacteria. Arthropods such as ticks, insects or mites are a group particularly favoured by the obligate intracellular bacteria. While arthropods are vectors for some of them, other bacteria inhabit invertebrate host cells having entered mutualistic interactions. Suc...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-006-0412-8
更新日期:2007-04-01 00:00:00
abstract::The DNA content of the developmental stages within the life cycle of Babesia divergens was measured by means of fluorescence microscopy using the DNA-specific bisbenzimide Hoechst 33258. By comparing the mean relative fluorescence intensities, the assumption of sexual reproduction in the gut of the tick vector (Ixodes...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00930815
更新日期:1990-01-01 00:00:00
abstract::The effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. This is due to the fact that some invasive mosquitoes, with special reference to the Aedes genus, are particularly difficult to control, due to their high ecological plasticity. Moreover, the indiscriminate over...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5395-0
更新日期:2017-04-01 00:00:00
abstract::We examined the in vitro effect of increasing gliotoxin concentrations on the infectivity of Toxoplasma gondii for NIH-3T3 murine fibroblasts and on Toxoplasma chymotrypsin-like activity, which is specific to the proteasome. Parasite penetration of host cells was not modified by a high gliotoxin concentration (1 micro...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0644-1
更新日期:2002-08-01 00:00:00
abstract::Hepatic enzymes and biochemical parameter values were analysed in the sera of 32 lambs: 12 infected with 1,000 Dicrocoelium dendriticum metacercariae, 12 with 3,000 and eight controls. Blood samples were collected at intervals of 30 days from day 0 post-infection (p.i.). Half the lambs in each group were slaughtered o...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1128-2
更新日期:2004-08-01 00:00:00
abstract::Neospora caninum is an intracellular protozoan that infects domestic and wild canids as well as many warm-blooded animals as shown by the isolation of viable parasites. The effectiveness of diagnostic tests for detecting specific antibodies against N. caninum is hampered by potential cross-reaction with other Coccidia...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3309-3
更新日期:2013-05-01 00:00:00
abstract::Angiostrongylus cantonensis invasion primarily cause heavy or negligible eosinophic meningitis and meningoencephalitis in the brain of non-permissive and permissive hosts, respectively. Chemokines are effective leukocyte chemoattractants and may play an essential role in mediating eosinophil recruitment in angiostrong...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3683-x
更新日期:2014-02-01 00:00:00
abstract::We analyzed extra chromosomal genomes of avian blood protozoa, Leucocytozoon caulleryi. One of the genomes, the mitochondrial genome was completely sequenced resulting 5,959 bp in length. This genome contained the identical gene organization and contents to that of other avian blood protozoa previously analyzed: three...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1083-4
更新日期:2008-09-01 00:00:00
abstract::One of the main drawbacks of experimental amebiasis is the lack of an adequate animal model for invasive intestinal lesions. Mongolian gerbils are useful because both intestinal and hepatic amebiasis can be produced experimentally with Entamoeba histolytica trophozoites. In this paper we show results obtained with in ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050228
更新日期:1997-01-01 00:00:00
abstract::Few data are available on the molecular characterization of Cryptosporidium spp. in cattle in China. In the present study, a total of 507 fecal specimens from six dairy farms in Heilongjiang Province were examined for Cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentatio...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1457-2
更新日期:2009-09-01 00:00:00
abstract::Myiasis refers to the infestation of living humans and vertebrates with fly larvae that feed on necrotic or vital tissue of the host. Since the invasion of new fly species in Europe is currently being observed, which live obligatorily parasitically or are close relatives of such species, the aim of this study is to ob...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6145-7
更新日期:2019-02-01 00:00:00
abstract::The anthelmintic potentials of the chloroform and methanol extracts of Buchholzia coriacea Engler seed were investigated. In folklore medicine, B. coriacea (Capparidaceae) is believed to be useful in the treatment of various kinds of ailments and diseases. At doses of 10 mg/kg, 25 mg/kg and 50 mg/kg, the extracts were...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2274-y
更新日期:2011-08-01 00:00:00
abstract::The complete coding sequence of manganese superoxide dismutase (Mn-SOD) of Trichinella pseudospiralis (Tp) was obtained and characterized for the first time by degenerative reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of complementary DNA ends (RACE) reactions. The open reading fram...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0755-9
更新日期:2008-01-01 00:00:00
abstract::Three specimens of Caudisoma durissa terrifica infected with Hepatozoon spp. were studied. One was parasitized by one type of gamont and the other two were each infected by two morphologically different gamonts. Utilizing morphology and morphometry analysis, we concluded that three types of gamonts were very similar a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2124-3
更新日期:2011-04-01 00:00:00
abstract::This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of P...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2514-1
更新日期:2012-02-01 00:00:00
abstract::From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket dragging at 45 sites all over the city area of Bonn, western Germany, to be checked for Borrelia burgdorferi infection. The collection sites included 20 private gardens, nine ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1240-3
更新日期:2005-01-01 00:00:00
abstract::This study investigates the occurrence of anisakids and raphidascarids in commercial fish from Balearic Sea (Western Mediterranean). A total of 335 fish including 19 black anglerfish (Lophius budegassa), 33 white anglerfish (L. piscatorius), 129 European hake (Merluccius merluccius), 30 red mullet (Mullus barbatus), a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6110-5
更新日期:2018-12-01 00:00:00
abstract::Trichomoniasis is the interplay between the infecting parasite, Trichomonas vaginalis, and the host, on which the clinical presentation of the disease depends. Although the clinical spectrum varies from an asymptomatic state to mild, moderate or severe symptoms, the exact virulence markers of T. vaginalis have not bee...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1155-z
更新日期:2004-09-01 00:00:00
abstract::Toxoplasma gondii uses a unique mechanism to fulfill its asexual life cycles by which the parasite can infect all the warm-blooded animals including humans. Mitogen-activated protein kinase (MAPK) or extracellular signal-regulated kinase (ERK) pathway widely existed in eukaryotic cells mediates the conversion of envir...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5103-5
更新日期:2016-09-01 00:00:00
abstract::In three different areas in northern Germany, hippoboscids were collected from red deer (Cervus elaphus). Typanosomatids were demonstrated in the midgut and hindgut of 9/37 Lipoptena cervi. The trypanosomatids were morphologically similar to vector forms of the subgenus Megatrypanum that had been found in tabanids at ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00928691
更新日期:1991-01-01 00:00:00
abstract::Visceral larva migrans (VLM) syndrome caused by Toxocara canis larvae was first described in the 1950s. The role of other nematode larvae, i.e. the pig roundworm Ascaris suum as a causative agent of visceral larva migrans-associated symptoms like general malaise, cough, liver dysfunction, hypereosinophilia with hepato...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4196-y
更新日期:2015-01-01 00:00:00
abstract::In 1997, an outbreak of alveolar echinococcosis in Japanese monkeys (Macaca fuscata) occurred in a zoo in Hokkaido, Japan. Twelve infected monkeys from a colony (n = 57) were diagnosed serologically by Western blotting, and ultrasonography showed the presence of tumor-like tissue in the livers of nine monkeys. The 12 ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1627-2
更新日期:2009-12-01 00:00:00
abstract::Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interac...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06887-x
更新日期:2020-11-01 00:00:00
abstract::There are first data on nematodes of Anas platyrhynchos Linnaeus, 1758 (mallard) and Bucephala clangula Linnaeus, 1758 (common goldeneye) from Northern Europe (Ladoga Lake region). The ducks were found to be infected with nine nematode species. A. platyrhynchos hosted eight nematode species and B. clangula was host to...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4697-3
更新日期:2015-10-01 00:00:00
abstract::cDNA clones for homologues of a molecular chaperone of the endoplasmic reticulum called the immunoglobulin heavy-chain binding protein (BiP) have been isolated from Eimeria maxima and E. tenella sporozoite cDNA libraries. The E. tenella cDNA clone is of full length and has a predicted N-terminal signal sequence of app...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050163
更新日期:1996-01-01 00:00:00