Abstract：
:The beta-tubulin coding sequences were investigated for the Chinese liver fluke, Clonorchis sinensis. A cDNA library of adult C. sinensis was screened with cDNA probes synthesized by polymerase chain reaction using degenerate primers, and two beta-tubulin cDNAs, CsTB1 and CsTB3, were subsequently cloned. The CsTB1 and CsTB3 cDNA were 2,082 and 1,486 bp long and encoded 445 and 444 amino acids, respectively. The two clones were identical 78% in their coding sequences and 97% in deduced sequences. Moreover, the CsTB1 had 3'-UTR 592 bp longer than that of CsTB3. The CsTB1 and CsTB3 polypeptides were highly homologous (88-99%) with those of known beta-tubulins of other helminthes with a variation at the C-terminal region. In CsTB1 and CsTB3 polypeptides, two amino acids Tyr 200 and Phe 167 were conserved. The phylogenetic analysis of selected sequences indicates that the beta-tubulins can be organized into five groups and that the two beta-tubulin isotypes of C. sinensis are contained within one group with those of other trematodes and vertebrates.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Li S,Hong SJ,Choi MH,Hong STdoi
10.1007/s00436-009-1512-zsubject
Has Abstractpub_date
2009-10-01 00:00:00pages
1015-21issue
4eissn
0932-0113issn
1432-1955journal_volume
105pub_type
杂志文章abstract：:Free-living amoebae (FLA) are protozoa ubiquitously found in nature. As some species or strains of these FLA are pathogenic for humans and animals, they represent objects of medical and parasitological research worldwide. Storage of valuable FLA strains in laboratories is often time- and energy-consuming and expensive...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6029-x
更新日期：2018-10-01 00:00:00
abstract：:The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence al...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0036-4
更新日期：2006-04-01 00:00:00
abstract：:We have identified the 2-Cys peroxiredoxin (Prx) from a bovine Babesia parasite, B. bovis. Prx is a recently described family of antioxidant enzymes that are highly conserved in eukaryotes and prokaryotes. B. bovis 2-Cys Prx (BbTPx-1) contained two conserved cysteine residues that corresponded to Cys47 and Cys170 of t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1587-6
更新日期：2009-10-01 00:00:00
abstract：:Paramyosin is a muscle protein that probably plays a role in the survival of the larval stage of Taenia solium during its prolonged host-parasite relationship. Here we describe the structure of the gene coding for the paramyosin of T. solium. The characterization of two clones obtained from a genomic library showed th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0761-x
更新日期：2003-03-01 00:00:00
abstract：:The complex life cycle of Toxoplasma gondii involves many animal species, raising zoonotic, economic, and conservation issues. This complexity is reflected in the molecular structure of T. gondii, whose different genotypes differ in pathogenicity. Among the intermediate hosts of T. gondii, wild ungulates may be a sour...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4981-x
更新日期：2016-05-01 00:00:00
abstract：:This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of P...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2514-1
更新日期：2012-02-01 00:00:00
abstract：:Two placebo-controlled pen studies were conducted to assess the efficacy of an imidacloprid 10 %/flumethrin 4.5 % polymer matrix collar (Seresto®, Bayer; Investigational Veterinary Product (IVP)) against the Australian paralysis tick (Ixodes holocyclus). Dogs assigned to the placebo (n = 8) or IVP (n ≥ 8) groups had c...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-013-3276-8
更新日期：2013-08-01 00:00:00
abstract：:A search for a protein(s) interacting with the block of proliferation 1 (Bop1) protein of Giardia lamblia led to the isolation of end binding (EB1) protein 1, a protein that is known to function as a microtubule (MT) plus-end-tracking protein in other organisms. Interaction between Bop1 and EB1 of G. lamblia was confi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1131-0
更新日期：2008-11-01 00:00:00
abstract：:Tungiasis is endemic in many countries in Latin America, the Caribbean and sub-Saharan Africa, and it is associated with severe morbidity. The pathophysiological and immunological characteristics of the ectoparasitosis are not well understood, and no effective therapy is currently available. The aim of this study was ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0731-4
更新日期：2007-12-01 00:00:00
abstract：:Chagas disease, caused by the protozoan Trypanosoma cruzi (T. cruzi), although discovered more than a century ago, is still a not very well-elucidated aspect. Individuals in the chronic phase of the disease may present asymptomatic clinical form or symptomatologies related to the cardiac, digestive systems, or both (m...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06377-9
更新日期：2019-08-01 00:00:00
abstract：:Argulus are common aquatic ectoparasites that create one of the major threats to aquaculture due to absence of suitable therapy. Piperine, a bioactive component of Piper longum, has medicinal properties and acts as anti-inflammatory, antibacterial, and antifungal, considering eco-friendliness and cost-effectiveness. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3054-z
更新日期：2012-11-01 00:00:00
abstract：:Infection in dogs and humans with the protozoan parasite Leishmania are widespread in tropical and subtropical countries around the globe. Sand flies of the order Phlebotomus in the Old World and Lutzomyia in the New World function as the vector of this disease. In dogs, skin lesions are the most prominent signs of ca...
journal_title：Parasitology research
pub_type： 临床试验,杂志文章
doi：10.1007/s00436-003-0905-7
更新日期：2003-07-01 00:00:00
abstract：:The purpose of this study was to genetically characterize and phylogenetically analyze the Cryptosporidium spp. isolated from exotic birds commercialized in popular markets, commercial aviaries, and pet shops located in Rio de Janeiro, Brazil. Fecal samples from individually housed birds were collected and subjected t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2636-5
更新日期：2012-04-01 00:00:00
abstract：:During our investigations on helminthofauna in roe deer (Capreolus capreolus) and red deer (Cervus elaphus) in France (1985-1998) we isolated nematodes not only of the genus Haemonchus Cobb, 1898 but also of the genus Ashworthius Le Roux 1930, both of which belong to the same subfamily of Haemonchinae. The prevalence ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008534
更新日期：2000-07-01 00:00:00
abstract：:Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by Cryptosporidium parvum, Cryptosporidium meleagridis, and Cryptosporidium baileyi with zoonotic potential have also been reported in domestic birds, and recent studies indicate the presence of new host-adapted species or genotypes in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1810-5
更新日期：2010-05-01 00:00:00
abstract：:Ticks are blood-feeding arthropods and can harbor several bacteria, including the worldwide zoonotic disease Q-fever agent Coxiella burnetii. Recent studies have reported a distinct group of Coxiella mostly associated with Ixodidae ticks, including the primary endosymbionts of Amblyomma americanum. In the present work...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5230-z
更新日期：2016-12-01 00:00:00
abstract：:In July 2005, 107 rainbow trout in age 1+ from a salmonid farm in Southern Germany situated in the southern tributary area of the Danube river were examined. The aim of this study was to determine the gyrodactylid species found on rainbow trout and to identify their location on the host's body. In total, 291 specimens...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1244-5
更新日期：2009-02-01 00:00:00
abstract：:It is well known that toxoplasmosis can be life threatening to immunocompromised individuals such as AIDS and organ transplantation patients. Glucocorticoids (GCs) are widely used in the clinic for the treatment of autoimmune diseases and organ transplantation resulting in acute toxoplasmosis in these patients. Howeve...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3661-3
更新日期：2014-01-01 00:00:00
abstract：:In frozen sections of the acanthocephalan Pomphorhynchus laevis, which is a frequent intestinal parasite of cyprinid and salmonid fishes, leucine aminopeptidase (APase) was localized histochemically in outer parts of the presomal bulbus as well as in all layers and most nuclei of the metasomal body wall. Enzyme activi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932697
更新日期：1994-01-01 00:00:00
abstract：:This paper reports the efficacy of a novel flavoured tablet formulation of emodepside plus praziquantel (Profender tablets for dogs) against mature and immature adult hookworms (Ancylostoma caninum and Uncinaria stenocephala) in dogs. The tablets were used at the minimum recommended dose of 1 mg emodepside and 5 mg pr...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-009-1490-1
更新日期：2009-08-01 00:00:00
abstract：:Ligictaluridus michaelalicea n. sp. (Monogenea: Dactylogyridae, Ancyrocephalinae) is described from the gills of Pylodictis olivaris (Siluriformes: Ictaluridae) from Wisconsin and Iowa portions of the upper Mississippi River. Diagnostic features include a relatively large, strongly curved tubular cirrus with minor ter...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5757-2
更新日期：2018-03-01 00:00:00
abstract：:In the present study, the morphology and morphometric characterization of Dujardinnascaris mujibii (Heterocheilidae) and Hysterothylacium aduncum (Anisakidae), new nematode parasites infecting the sea bream Pagrus pagrus (Osteichthyes, Sparidae), were described for the first time from the Gulf of Suez and Hurghada Cit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3270-6
更新日期：2013-02-01 00:00:00
abstract：:Hepatic enzymes and biochemical parameter values were analysed in the sera of 32 lambs: 12 infected with 1,000 Dicrocoelium dendriticum metacercariae, 12 with 3,000 and eight controls. Blood samples were collected at intervals of 30 days from day 0 post-infection (p.i.). Half the lambs in each group were slaughtered o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1128-2
更新日期：2004-08-01 00:00:00
abstract：:The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0541-8
更新日期：2007-08-01 00:00:00
abstract：:Intraspecific variation in Schistosoma mansoni infection and modulation of its expression by vertebrate host genetics was studied by evaluation of some biological parameters of the infection in BALB/c and C57BL/6 mice infected with one Brazilian (BH) and two Venezuelan (YT and SM) laboratory strains of the parasite. M...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008565
更新日期：2001-02-01 00:00:00
abstract：:A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 inte...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1006-3
更新日期：2003-12-01 00:00:00
abstract：:A 1-month-old brown wood owlet (Strix leptogrammica) purchased from a wholesaler and housed as a companion bird by an individual owner in Japan showed severe dehydration and anorexia following a week of vomiting and severe diarrhea. A great number of approximately 5 × 4-μm-sized Cryptosporidium oocysts were found in t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5951-2
更新日期：2018-09-01 00:00:00
abstract：:Two of the three vector species of Schistosoma mansoni Sambon, 1907 in Brazil occur in the state of Maranhão: Biomphalaria glabrata (Say, 1818) and Biomphalaria straminea (Dunker, 1848). For the implementation of effective measures to combat schistosomiasis, it is necessary to identify the spatial and seasonal dynamic...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5810-1
更新日期：2018-05-01 00:00:00
abstract：:Many factors contribute to parasites varying in host specificity and distribution among potential hosts. Metagonimoides oregonensis is a digenetic trematode that uses stream-dwelling plethodontid salamanders as second intermediate hosts in the Eastern US. We completed a field survey to identify which stream salamander...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4550-8
更新日期：2015-08-01 00:00:00
abstract：:In the present study, we sequenced and analyzed the mitochondrial (mt) genome of Gastrothylax crumenifer and compared it with other selected trematodes. The full mt genome of G. crumenifer was amplified, sequenced, assembled, analyzed and then subjected to phylogenetic analysis. The complete mt genome of G. crumenifer...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5019-0
更新日期：2016-06-01 00:00:00
