Abstract：
:Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the specific antigen genes was investigated. The study included 164 children presenting with mild and severe malaria from central Uganda where malaria is meso-endemic. The polymorphic regions of the circumsporozoite protein (csp), merozoite surface proteins 1 and 2 (msp1 and msp2), and glutamate-rich protein (glurp) were genotyped by polymerase chain reaction methods and fragment analysis by gel electrophoresis. In a subset of samples fragment analysis was also performed by fluorescent PCR genotyping followed by capillary electrophoresis. The multiplicity of infection (MOI), determined as the highest number of alleles detected within any of the four genetic loci, was significantly higher in severe than in mild malaria cases (mean 3.7 and 3.0, respectively, P=0.002). No particular genotype or allelic family of msp1 or msp2 was associated with severity of malaria, and nor did the genotyping method reveal any significant difference in MOI when only assessed by msp2 genotyping. Severity of malaria was not linked to the predominance of any particular msp1 or msp2 allelic types, independent of methods used for genotyping. Monitoring the dynamics of multiple clone infections in relation to disease outcome, host immune status and genetic factors will provide more insight into parasite virulence mechanisms.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Kiwuwa MS,Ribacke U,Moll K,Byarugaba J,Lundblom K,Färnert A,Fred K,Wahlgren Mdoi
10.1007/s00436-013-3325-3subject
Has Abstractpub_date
2013-04-01 00:00:00pages
1691-700issue
4eissn
0932-0113issn
1432-1955journal_volume
112pub_type
杂志文章abstract：:Non-invasive diagnoses for most of the extraintestinal helminth infections among vertebrates are rare. In the present study, we developed and tested a non-invasive visual diagnosis method to detect the infection of the nematode Philometra ovata, which is parasitising in the body cavity of the common minnow Phoxinus ph...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3099-z
更新日期：2012-12-01 00:00:00
abstract：:In the present study the behavior and pathogenicity of second-stage larvae of Toxocara canis were examined in different mouse strains with special emphasis on the major histocompatibility complex (MHC). Mice of the inbred strains BALB, C3H, C57BL, and DBA and the outbred strain NMRI were infected orally with 1000 seco...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932955
更新日期：1994-01-01 00:00:00
abstract：:This study was conducted to identify genetic characteristics of Besnoitia spp. isolated from goat in Iran. Molecular analysis of two portions of nuclear ribosomal DNA (ITS1 and ITS2) was used for the genetic characterization of the Besnoitia species. Comparison of the sequencing data of the Iranian Besnoitia samples o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2107-4
更新日期：2011-03-01 00:00:00
abstract：:Male Sprague-Dawley rats were dosed orally with nitroxynil at a concentration of 40 mg/kg and adult Fasciola hepatica recovered after 24 h, 48 h and 72 h. Surface changes to the flukes were monitored by means of SEM. After the 24 h treatment, extensive swelling and blebbing of the tegument was observed on both surface...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-0930-6
更新日期：2003-10-01 00:00:00
abstract：:Eimeria tenella-specific antibodies were examined for the cross-reactivity on the sporozoites and merozoites of E. tenella, Eimeria maxima, Eimeria acervulina and Eimeria brunetti in an indirect fluorescence antibody test. Two of nine antibodies showed cross-reactivity with sporozoites of E. maxima, E. acervulina and ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2171-9
更新日期：2011-03-01 00:00:00
abstract：:The existing armament of drugs for the treatment and prevention of malaria is inadequate due to development of resistance. In addition to this due to lack of economic enticement the rate of new drug development and new drug discovery in the segment of parasitic diseases is very low as compared to the other segments. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1789-y
更新日期：2010-05-01 00:00:00
abstract：:Results obtained in experiments testing the efficacy of anti-procyclic-form rabbit sera on the development of homologous and heterologous stocks of Trypanosoma brucei brucei in Glossina morsitans morsitans indicated that this development was affected little, or not at all, by such sera. The absence of effect of anti-p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00934382
更新日期：1991-01-01 00:00:00
abstract：:The progressive visceral infection caused in golden hamsters by Leishmania donovani amastigotes led to gradual impairment of the proliferative response of their splenic (SPMC) or peripheral blood (PBMC) mononuclear cells to in vitro stimulation with leishmanial antigen, with mitogen (concanavalin A), and even with a c...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050600
更新日期：1999-07-01 00:00:00
abstract：:Host feeding patterns of engorged sibling species of the Culicoides obsoletus and Culicoides pulicaris groups captured during three nights on two selected farms maintaining either cattle, sheep, horses, and pigs (Seedorf, Brandenburg) or cattle, sheep, moufflons, and red and fallow deer (Paulinenaue, Brandenburg) were...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1408-y
更新日期：2009-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06699-z
更新日期：2020-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1397-x
更新日期：2009-06-01 00:00:00
abstract：:The first description of an electron microscopic study of Trypanosoma corvi in the vector Ornithomyia avicularia is reported. There is a close association between vector and parasite in the midgut, ileum and rectum of the vector. The midgut distribution of parasites is determined by the peritrophic membrane, which con...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931269
更新日期：1989-01-01 00:00:00
abstract：:In many helminthic infections, eotaxin, a CC-chemokine, triggers the mobilization of eosinophils, thus, contributing to an elevated blood and periparasitic eosinophil level. Following an experimental intraperitoneal infection of C57BL6 mice with Echinococcus multilocularis metacestodes, however, we observed the absenc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1549-z
更新日期：2009-10-01 00:00:00
abstract：:The present report describes a method for establishment of colonies of Blastocystis hominis from single cells in soft agar. The percentage of colony-forming efficiency (% CFE = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. Fiv...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050194
更新日期：1996-01-01 00:00:00
abstract：:Two thousand four hundred fifty-eight fish comprised of 53 species were captured in the Danjiangkou Reservoir, in the northwestern part of Hubei Province, central China during 2004, to examine Camallanus cotti infections. We found that 19 cypriniform, 3 siluriforme, and 4 perciforme fishes were infected by the nematod...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0472-4
更新日期：2007-07-01 00:00:00
abstract：:Echinostoma trivolvis adults grown in golden hamsters were treated in vitro with [3H]-tyrosine to label sperm and transplanted to uninfected hamsters alone or with four to six unlabeled adults. After 4-5 days, worms were recovered, processed for autoradiography, and observed for silver grains over their seminal recept...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932258
更新日期：1993-01-01 00:00:00
abstract：:The C57BL/6J strain of Mus musculus is susceptible to the terrestrial trematode Brachylaima cribbi. The duration of infection in these mice is generally 9-12 weeks with a peak excretion of eggs at 4 weeks post-infection (wpi). The effects of age and sex on the course of infection were investigated by comparing infecti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0642-3
更新日期：2002-07-01 00:00:00
abstract：:Passive transfer of immunity using a mouse monoclonal IgE antibody against Schistosoma japonicum was found to be enhanced by heterologous helminth infections. BALB/c mice were infected with Toxocara canis or Nippostrongylus brasiliensis so as to induce eosinophilia prior to a challenge infection with S. japonicum. Rec...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00928681
更新日期：1991-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0665-9
更新日期：2002-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3421-4
更新日期：2013-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2665-0
更新日期：2012-04-01 00:00:00
abstract：:On the basis of information acquired from local health authorities in Evora district of Portugal on cases of visceral leishmaniasis (VL), an epidemiology survey study was conducted. To determine the prevalence of anti-Leishmania antibodies in the local human and canine populations residing in Evora town and 14 adjacen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00937115
更新日期：1995-01-01 00:00:00
abstract：:Toxocariasis is a worldwide zoonotic disease caused by the ascarid nematode Toxocara canis. The most common method available for serodiagnosis of toxocariasis is an enzyme-linked immunosorbent assay (ELISA) test using Toxocara excretory-secretory antigen (TES). The present study describes the development of IgG-ELISA ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1067-4
更新日期：2008-09-01 00:00:00
abstract：:Following infection of rats with Angiostrongylus cantonensis, occurrence of anti-parasite antibody in the serum was determined with special reference to the antigens recognized by host IgG antibodies, using SDS-PAGE combined with an immunoblotting technique. Three saline extracts of digestive organ, reproductive organ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00531092
更新日期：1987-01-01 00:00:00
abstract：:Leishmania has distinct epidemiological and biological characteristics and causes a variety of clinical symptoms. To understand the genetic diversity and the phylogenetic relationships among Leishmania isolates from China, 29 Leishmania isolates from different geographic origins, vectors, and hosts were analyzed using...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1814-1
更新日期：2010-05-01 00:00:00
abstract：:Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050568
更新日期：1999-05-01 00:00:00
abstract：:To elucidate whether Kudoa septempunctata was able to live in the human intestine, we assessed viability of K. septempunctata sporoplasms under conditions that mimicked human and ragworm digestive tracts. To study the effect of osmotic pressure on viability, sporoplasms were incubated in 0.9 or 3.4 % sodium chloride s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5036-z
更新日期：2016-06-01 00:00:00
abstract：:In temperate regions, some avian haemosporidian parasites have evolved seasonal transmission strategies, with chronic infections relapsing during spring and transmission peaking during the hosts' breeding season. Because lineages with seasonal transmission strategies are unlikely to produce gametocytes in winter, we p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06646-y
更新日期：2020-05-01 00:00:00
abstract：:Hepatozoon canis is a hemoprotozoan organism that infects domestic and wild carnivores throughout much of Europe. The parasite is mainly transmitted through the ingestion of infected ticks containing mature oocysts. The aims of the present survey were to determine the prevalence of H. canis in hunting dogs living in S...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06820-2
更新日期：2020-09-01 00:00:00
abstract：:The effect of pentavalent meglumine antimoniate (glucantime) on the growth kinetics of promastigotes of 13 South American Leishmania strains isolated from patients, sylvatic reservoirs, and vectors was studied. Four of five L. (Viannia) braziliensis human isolates were obtained from drug-responsive patients and one wa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050476
更新日期：1998-09-01 00:00:00
