Molecular characterization of Leishamania isolates from China by inter-simple sequence repeat polymerase chain reaction.

Abstract:

:Leishmania has distinct epidemiological and biological characteristics and causes a variety of clinical symptoms. To understand the genetic diversity and the phylogenetic relationships among Leishmania isolates from China, 29 Leishmania isolates from different geographic origins, vectors, and hosts were analyzed using 21 inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) primers. A total of 864 polymorphic bands were obtained. According to the results of the neighbor-joining phylogenetic tree and principal component analysis, the 29 isolates studied clustered into six groups. Isolates of Leishmania donovani complex from China share the highest similarity with the reference strain of L. donovani (DD8). This study helps to elucidate the genetic relationship among Leishmania isolates from China and similarities between Chinese isolates and World Health Organization reference strains. Furthermore, ISSR-PCR could also be a quick, simple, and reliable method for Leishmania species identification.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Wang Y,Yang Y,Wang J,Bao Y,Guan L,Gao C,Shi F

doi

10.1007/s00436-010-1814-1

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

1385-94

issue

6

eissn

0932-0113

issn

1432-1955

journal_volume

106

pub_type

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