Abstract:
:Leishmania has distinct epidemiological and biological characteristics and causes a variety of clinical symptoms. To understand the genetic diversity and the phylogenetic relationships among Leishmania isolates from China, 29 Leishmania isolates from different geographic origins, vectors, and hosts were analyzed using 21 inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) primers. A total of 864 polymorphic bands were obtained. According to the results of the neighbor-joining phylogenetic tree and principal component analysis, the 29 isolates studied clustered into six groups. Isolates of Leishmania donovani complex from China share the highest similarity with the reference strain of L. donovani (DD8). This study helps to elucidate the genetic relationship among Leishmania isolates from China and similarities between Chinese isolates and World Health Organization reference strains. Furthermore, ISSR-PCR could also be a quick, simple, and reliable method for Leishmania species identification.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Wang Y,Yang Y,Wang J,Bao Y,Guan L,Gao C,Shi Fdoi
10.1007/s00436-010-1814-1subject
Has Abstractpub_date
2010-05-01 00:00:00pages
1385-94issue
6eissn
0932-0113issn
1432-1955journal_volume
106pub_type
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