Abstract:
:Cysteine proteases of parasite organisms play numerous indispensable roles in tissue penetration, feeding, immunoevasion, virulence, egg hatching and metacercarial excystment. They are critical key enzymes in the biology of parasites and have been exploited as serodiagnostic markers, therapeutic and vaccine targets. In the present study, the cysteine proteases in the in vitro released excretory/secretory (E/S) products of the digenetic trematode parasite, Euclinostomum heterostomum have been analysed. The encysted progenetic metacercariae of E. heterostomum collected from the infected liver and kidney of Channa punctatus were excysted in vitro and incubated in phosphate buffer at 37 ± 1 °C, and the E/S products released were analysed. The spectrophotometric analysis of the proteases revealed active hydrolysis of chromogenic substrate, azocoll, in a time-, temperature- and pH-dependent manner. Optimum activity was observed at pH 7.0 at 37 ± 1 °C, and with 1 mM each of various protease inhibitors (Mini Protease Inhibitor Cocktail, ethylene diaminetetraacetic acid, phenyl methyl sulphonyl fluoride, iodoacetamide and 1,10-phenanthroline) used, significant inhibition was observed by iodoacetamide and 85% of inhibition at a concentration of 2 mM, suggesting that cysteine protease is a major component in the E/S of this parasite. Four discrete protease bands of Mr 36, 39, 43 and 47 kDa were identified by gelatin-substrate zymography. Maximum gelatinolytic activity was observed at pH 7.0, and among various inhibitors used, almost complete disappearance of protease bands was observed by 2 mM iodoacetamide. The proteolytic cleavage of bovine serum albumin, bovine haemoglobin and human haemoglobin in vitro were also studied.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Shareef PA,Abidi SMdoi
10.1007/s00436-013-3627-5subject
Has Abstractpub_date
2014-01-01 00:00:00pages
65-71issue
1eissn
0932-0113issn
1432-1955journal_volume
113pub_type
杂志文章abstract::Triatoma platensis is occasionally found coexisting with Triatoma infestans in chicken coops in Argentina. Some authors have reported the presence of hybrid specimens of both species in chicken coops and other peridomestic habitats. Given the coexistence of T. infestans with T. platensis and the possibility of generat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06822-0
更新日期:2020-09-01 00:00:00
abstract::Equine ocular setariasis arising mainly from ectopic infestation of Setaria digitata is a common vision impairing ophthalmic disease in India, and the identification of this filarial nematode is based solely on morphology. However, morphological characters alone are inadequate to detect and differentiate S. digitata f...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06587-1
更新日期:2020-02-01 00:00:00
abstract::The parasite fauna of the stone loach (Barbatula barbatula) was investigated monthly from May to December 2001. Four parasite species were recorded: Gyrodactylus spp. (Monogenea), Tylodelphys clavata (Digenea), Proteocephalus sagittus (Cestoda), and Raphidascaris acus (Nematoda). The changes in the seasonal dynamics o...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1261-y
更新日期:2005-01-01 00:00:00
abstract::An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its eff...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0661-0
更新日期:2002-08-01 00:00:00
abstract::The insect growth regulator, Lufenuron, at concentrations of even multiples of LC(90) (determined under laboratory conditions) was tested against the III instar larvae of Aedes aegypti under simulated field conditions. For all concentrations tested, 100 % mortality of the larvae was observed within 24 h of exposure to...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-2968-9
更新日期:2012-09-01 00:00:00
abstract::An endopeptidase specific to the Plasmodium falciparum erythrocytic schizont stage and to free merozoites was detected using the fluorogenic GlcA-Val-Leu-Gly-Lys(or Arg)-AEC substrate. The enzyme was purified by high performance liquid chromatography (HPLC); its optimal activity was around pH 7.5 and its isoelectric p...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00930972
更新日期:1989-01-01 00:00:00
abstract::A study was made to determine the prevalence of camel trypanosomosis (surra) and its associated risk factors in Borena zone, southern Ethiopia during 2013-2014. A total of 2400 blood samples were collected and examined by the buffy coat and thin blood film laboratory methods, and data were analyzed using the SPSS stat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4845-9
更新日期:2016-03-01 00:00:00
abstract::Radiological features and biochemical changes were investigated during the parenchymal and ductal phases of chronic Fasciola hepatica infection in sheep. The activities of plasma aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and gamma-glutamyl transpeptidase (GGT), plasma levels of IgG anti- F. hepatic...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-0866-x
更新日期:2003-08-01 00:00:00
abstract::The progressive visceral infection caused in golden hamsters by Leishmania donovani amastigotes led to gradual impairment of the proliferative response of their splenic (SPMC) or peripheral blood (PBMC) mononuclear cells to in vitro stimulation with leishmanial antigen, with mitogen (concanavalin A), and even with a c...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050600
更新日期:1999-07-01 00:00:00
abstract::In nature, parasitic infections must be addressed as complex systems involving parasite-host relationships on a temporal and spatial scale. Since the parasites cover a great biological diversity, we can expect that wildlife are exposed simultaneously to different parasites. In this sense, the objective of this work wa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-07028-0
更新日期:2021-01-09 00:00:00
abstract::Myxobolus miyairii Kudo, 1919 was first reported from the intestines of the Amur catfish (Silurus asotus) in Japan and then in China and Russia, but with incomplete description. During the investigation of fish myxosporean diversity in Poyang Lake, the biggest lake along the Yangtze River, China, two Amur catfish high...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4889-x
更新日期:2016-04-01 00:00:00
abstract::This report describes for the first time the occurrence of alveolar echinococcosis in two exotic rodent species in Europe. A pet chinchilla (Chinchilla laniger) was euthanized due to a painful enlargement of the abdominal cavity, and a Prevost's squirrel (Callosciurus prevostii borneoensis) was found dead in the enclo...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0717-2
更新日期:2007-11-01 00:00:00
abstract::Seventy specimens of Hypsiboas albopunctatus (Anura: Hylidae) were collected and surveyed for endoparasites in Brasília, Brazil at the end of the rainy season (February and March) in 2005. Sixteen males (22.86%) were infected by Rhabdias sp., Aplectana sp., Cosmocerca sp., and Falcaustra mascula. Rhabdias sp., a lung ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0797-z
更新日期:2008-03-01 00:00:00
abstract::Two of the three vector species of Schistosoma mansoni Sambon, 1907 in Brazil occur in the state of Maranhão: Biomphalaria glabrata (Say, 1818) and Biomphalaria straminea (Dunker, 1848). For the implementation of effective measures to combat schistosomiasis, it is necessary to identify the spatial and seasonal dynamic...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5810-1
更新日期:2018-05-01 00:00:00
abstract::Immunocytochemical studies using antibodies against various peptides were performed on Echinostoma caproni. Only the presence of substance P or a related peptide was clearly demonstrated in this digenetic trematode. In the central nervous system, substance P-immunoreactive neurons were few and constituted two populati...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00930963
更新日期:1989-01-01 00:00:00
abstract::Analyses were made on the adenosine transporter-1 gene in Trypanosoma brucei (TbAT1), encoding a P2-like nucleoside transporter, from T. brucei brucei field stocks to investigate a possible link between the presence of mutations in this gene and isometamidium resistance. We have analysed the gene from 11 isometamidium...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0141-z
更新日期:2006-08-01 00:00:00
abstract::Host feeding patterns of engorged sibling species of the Culicoides obsoletus and Culicoides pulicaris groups captured during three nights on two selected farms maintaining either cattle, sheep, horses, and pigs (Seedorf, Brandenburg) or cattle, sheep, moufflons, and red and fallow deer (Paulinenaue, Brandenburg) were...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1408-y
更新日期:2009-08-01 00:00:00
abstract::The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5539-2
更新日期:2017-08-01 00:00:00
abstract::A seasonal investigation on the occurrence of fishborne zoonotic trematodes (FZT) in economically important mono-cultured hybrid catfish and giant gouramy was conducted in the Mekong Delta of Vietnam. Fish from carp poly-culture and intensive small-scale integrated vegetable-aquaculture-animal husbandry farming (VAC) ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0633-5
更新日期:2007-10-01 00:00:00
abstract::Physaloptera rara (Spirurida: Physalopteridae) has been found in dogs, coyotes, raccoons, wolves, foxes, cats, and bobcats in North America. The parasites' developmental cycles involve insects, including beetles, cockroaches, and crickets, as intermediate hosts. The nematodes firmly attach to the wall of the stomach a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3356-9
更新日期:2013-05-01 00:00:00
abstract::This study is part of an effort to document the diversity of avian schistosomes in ducks and snails in Northern Iran, a major flyway (Black Sea/Mediterranean) for migratory birds and where cercarial dermatitis (CD) is prevalent in rice growing areas. CD is an allergic skin reaction from schistosome trematodes that eme...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6087-0
更新日期:2018-12-01 00:00:00
abstract::In forensic investigations, immature stages of the fly (egg, larva, or puparia) can be used as entomological evidence at death scenes, not only to estimate the postmortem interval (PMI), analyze toxic substances, and to determine the manner of death but also to indicate the movement of a corpse in homicide cases. Of t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0660-2
更新日期:2007-10-01 00:00:00
abstract::While Gobioidei comprises showcases of (adaptive) radiation, the scientific interest they yielded did not ensure full understanding of goby biodiversity. Even in a well-studied region like Europe, wide knowledge gaps remain. Sand gobies represent one of the few clades whose monogenean parasites have been thoroughly st...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3693-8
更新日期:2014-02-01 00:00:00
abstract::We examined the in vitro effect of increasing gliotoxin concentrations on the infectivity of Toxoplasma gondii for NIH-3T3 murine fibroblasts and on Toxoplasma chymotrypsin-like activity, which is specific to the proteasome. Parasite penetration of host cells was not modified by a high gliotoxin concentration (1 micro...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0644-1
更新日期:2002-08-01 00:00:00
abstract::We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blas...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1006-4
更新日期:2008-08-01 00:00:00
abstract::Hookworm infection is still prevalent in southern Thailand despite control measures. Hookworm eggs submerged for an extended period under water from rainfall or in latrines may not survive, but they may recover their ability to develop into infective larvae when exposed to atmospheric air. This study examined the surv...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5031-4
更新日期:2016-07-01 00:00:00
abstract::This study was conducted to evaluate the efficacy of a new topical ectoparasiticidal spot-on containing 4.95% dinotefuran (w/w), 36.08% permethrin (w/w) and 0.44% pyriproxyfen (w/w) (Vectra 3D, Ceva, Libourne, France) against Portuguese strain of Phlebotomus perniciosus and a French strain of Ctenocephalides canis in ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3568-z
更新日期:2013-11-01 00:00:00
abstract::Previous studies have shown that excretory/secretory products of larval Taenia crassiceps have immunomodulatory activities. We report here that one of these products, termed p66, possesses activities that mimic some characteristics of murine interferon-gamma (IFN-gamma). Purified p66 cross-reacts with anti-murine IFN-...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0590-y
更新日期:2002-05-01 00:00:00
abstract::Ruminant coccidiosis is a gastrointestinal disease caused by parasites of the genus Eimeria. Environmental and climatic factors are relevant for the development, survival, and transmission of coccidiosis because Eimeria oocysts are able to survive in the environment for several weeks or months in favorable conditions ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06548-8
更新日期:2020-03-01 00:00:00
abstract::Little information is available on the occurrence of endoparasites in pet guinea pigs (Cavia porcellus). The purpose of this study was to evaluate the prevalence of intestinal parasites in cavies kept as pets in southern Italy. Fresh fecal samples were randomly collected from 60 guinea pigs housed in pet shops or priv...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4289-7
更新日期:2015-03-01 00:00:00