Abstract：
:Trypanosoma cruzi can modulate a large number of host intracellular responses during its invasion. GTPases such as RhoA, Rac1 and Cdc42 are examples of molecules that could be activated at this moment and trigger changes in the pattern of F-actin cytoskeleton leading to the formation of structures like stress fibers, lamellipodium and fillopodium, respectively. Here we investigate the role of these GTPases in the cytoskeletal rearrangement of MDCK cell transfectants expressing variants of RhoA, Rac1 and Cdc42 during T. cruzi infection. The adhesion, internalization and the survival rate were determined. Rac1 mutants showed the higher adhesion and internalization indexes but the lower survival index after 48 h of infection. Confocal laser scanning microscopy showed changes in the pattern of F-actin distribution and reorganization at the site of trypomastigote invasion. These observations suggest that these GTPases act in the signaling mechanisms that affect the F-actin cytoskeleton during T. cruzi invasion.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Dutra JM,Bonilha VL,De Souza W,Carvalho TMdoi
10.1007/s00436-005-1333-7subject
Has Abstractpub_date
2005-06-01 00:00:00pages
171-7issue
3eissn
0932-0113issn
1432-1955journal_volume
96pub_type
杂志文章abstract：:We assessed the cellular immunological responses to two Plasmodium falciparum liver-stage antigen (LSA)-1-derived T-cell epitopes in healthy Gabonese children and adults. The N-terminal peptide, designated T1, induced interferon (IFN)-gamma production in peripheral blood mononuclear cells (PBMC) from a significantly l...
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pub_type： 临床试验,杂志文章,随机对照试验
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