Simultaneous identification and DNA barcoding of six Eimeria species infecting turkeys using PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus.

Abstract:

:Species-specific PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus were generated that allow for the specific identification of the most common Eimeria species infecting turkeys (i.e., Eimeria adenoeides, Eimeria meleagrimitis, Eimeria gallopavonis, Eimeria meleagridis, Eimeria dispersa, and Eimeria innocua). PCR reaction chemistries were optimized with respect to divalent cation (MgCl2) and dNTP concentrations, as well as PCR cycling conditions (particularly anneal temperature for primers). Genomic DNA samples from single oocyst-derived lines of six Eimeria species were tested to establish specificity and sensitivity of these newly designed primer pairs. A mixed 60-ng total DNA sample containing 10 ng of each of the six Eimeria species was used as DNA template to demonstrate specific amplification of the correct product using each of the species-specific primer pairs. Ten nanograms of each of the five non-target Eimeria species was pooled to provide a non-target, control DNA sample suitable to test the specificity of each primer pair. The amplifications of the COI region with species-specific primer pairs from pooled samples yielded products of expected sizes (209 to 1,012 bp) and no amplification of non-target Eimeria sp. DNA was detected using the non-target, control DNA samples. These primer pairs specific for Eimeria spp. of turkeys did not amplify any of the seven Eimeria species infecting chickens. The newly developed PCR primers can be used as a diagnostic tool capable of specifically identifying six turkey Eimeria species; additionally, sequencing of the PCR amplification products yields sequence-based genotyping data suitable for identification and molecular phylogenetics.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Hafeez MA,Shivaramaiah S,Dorsey KM,Ogedengbe ME,El-Sherry S,Whale J,Cobean J,Barta JR

doi

10.1007/s00436-015-4361-y

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

1761-8

issue

5

eissn

0932-0113

issn

1432-1955

journal_volume

114

pub_type

杂志文章
  • Antimalarial activity of Bergenia ciliata (Haw.) Sternb. against Plasmodium berghei.

    abstract::The emergence of resistance against most of the drugs in current use against malaria has aggravated the disease burden in endemic regions. Several plants species have been used for treatment of malaria in traditional/cultural health systems. Bergenia ciliata, used traditionally for treatment of fever by local communit...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3487-z

    authors: Walter NS,Bagai U,Kalia S

    更新日期:2013-09-01 00:00:00

  • Ultrastructural observations on the elusive subtegumental cells of the viviparous gill monogenean, Macrogyrodactylus clarii.

    abstract::The ultrastructure of the tegument, including putative subtegumental cells, of the monogenean gill parasite Macrogyrodactylus clarii is described for the first time. The lateral cells are secretory in nature and can be classified into four types: T1 producing elongated, electron-dense, bodies (t1); T2 manufacturing el...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-006-0448-9

    authors: El-Naggar MM,Cable J

    更新日期:2007-06-01 00:00:00

  • Description of gamontogonic and sporogonic stages of Hepatozoon spp. (Apicomplexa, Hepatozoidae) from Caudisoma durissa terrifica (Serpentes, Viperidae).

    abstract::Three specimens of Caudisoma durissa terrifica infected with Hepatozoon spp. were studied. One was parasitized by one type of gamont and the other two were each infected by two morphologically different gamonts. Utilizing morphology and morphometry analysis, we concluded that three types of gamonts were very similar a...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-2124-3

    authors: O'Dwyer LH,da Silva RJ,Madeira NG

    更新日期:2011-04-01 00:00:00

  • Echinococcus multilocularis (Cestoda, Cyclophyllidea, Taeniidae): functional ultrastructure of the penetration glands and nerve cells within the oncosphere.

    abstract::The fine structure of the infective hexacanths of Echinococcus multilocularis was examined with particular emphasis on the functional ultrastructure of penetration glands and nerve cells directly involved in the mechanism of initial host infection. The oncosphere contains two types of penetration glands, PG1 and PG2, ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-018-5957-9

    authors: Świderski Z,Miquel J,Azzouz-Maache S,Pétavy AF

    更新日期:2018-08-01 00:00:00

  • Occurrence of Leishmania infantum and associated histological alterations in the genital tract and mammary glands of naturally infected dogs.

    abstract::The objectives of this study were to evaluate the occurrence of Leishmania infantum in the male and female genital tract and female mammary glands of dogs and the parasite burden and to identify histological alterations associated with this protozoan. Twenty male and 20 female Leishmania-seropositive dogs with isolati...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-4987-4

    authors: Boechat VC,Mendes Junior AA,Madeira Mde F,Ferreira LC,Figueiredo FB,Rodrigues Fd,Oliveira Vda C,de Oliveira Rde V,Menezes RC

    更新日期:2016-06-01 00:00:00

  • Abortive long-lasting sporogony of two Haemoproteus species (Haemosporida, Haemoproteidae) in the mosquito Ochlerotatus cantans, with perspectives on haemosporidian vector research.

    abstract::Haemoproteus spp. are cosmopolitan vector-born haemosporidian parasites, some species of which cause diseases in non-adapted birds. Recent polymerase chain reaction (PCR)-based studies have detected mitochondrial cytochrome b gene lineages of these Haemoproteus parasites in blood-sucking mosquitoes and speculated abou...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3375-6

    authors: Valkiūnas G,Kazlauskienė R,Bernotienė R,Palinauskas V,Iezhova TA

    更新日期:2013-06-01 00:00:00

  • Effects of parasitic helminths and ivermectin treatment on clinical parameters in the European wild boar (Sus scrofa).

    abstract::Limited information exists on serum biochemistry and haematology of the European wild boar, and few correlations have been found between parasitic burden and clinical parameters in this species. Naturally infected wild boars were experimentally treated to study the effect of nematode parasites and ivermectin treatment...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-0099-2

    authors: López-Olvera JR,Höfle U,Vicente J,Fernández-de-Mera IG,Gortázar C

    更新日期:2006-05-01 00:00:00

  • Prevalence of helminths in horses in the state of Brandenburg, Germany.

    abstract::The objective of the study was to estimate the prevalence of helminths in the horse population of the state of Brandenburg, Germany. One hundred and twenty-six horse farms in the state were selected by randomised stratified sampling. In total, 1,407 horses across all farms were examined coproscopically. The experiment...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2362-z

    authors: Hinney B,Wirtherle NC,Kyule M,Miethe N,Zessin KH,Clausen PH

    更新日期:2011-05-01 00:00:00

  • Biological and molecular features of Nosema rachiplusiae sp. n., a microsporidium isolated from the neotropical moth Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae).

    abstract::Light, electron microscopy and DNA analyses were performed to characterize a microsporidium infecting Rachiplusia nu larvae from a laboratory rearing in Argentina. Diplokaryotic spores were oval and measured 3.61 ± 0.29 × 1.61 ± 0.14 μM (fresh). The spore wall was composed of an electron-dense exospore and an electron...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-018-5820-z

    authors: Arneodo JD,Sciocco-Cap A

    更新日期:2018-05-01 00:00:00

  • Fasciola hepatica: disruption of spermatogenesis by the fasciolicide compound alpha.

    abstract::Sheep infected with the triclabendazole-susceptible Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48, and 72 h post-treatment (p.t.). Changes to the spermatogenic cells in the testis were examined by...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-009-1649-9

    authors: McConville M,Hanna RE,Brennan GP,McCoy M,Edgar HW,McConnell S,Castillo R,Hernández-Campos A,Fairweather I

    更新日期:2010-01-01 00:00:00

  • Comparative Efficacy of an Imidacloprid/Flumethrin Collar (Seresto®) and an Oral Afoxolaner Chewable (NexGard®) against Tick (Dermacentor variabilis and Amblyomma americanum) Infestations on Dogs: a Randomised Controlled Trial.

    abstract::This randomised controlled laboratory study demonstrated the residual speed of efficacy of an imidacloprid/flumethrin collar (Seresto(®), Bayer) for the control of ticks (Dermacentor variabilis, Amblyomma americanum) at 6 and 12 hours postinfestation on dogs when compared to oral afoxolaner (NexGard(®), Merial). Dogs ...

    journal_title:Parasitology research

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00436-015-4515-y

    authors: Ohmes CM,Hostetler J,Davis WL,Settje T,Everett WR

    更新日期:2015-08-01 00:00:00

  • An identical epitope in Pneumocystis carinii and Toxoplasma gondii causing serological cross reactions.

    abstract::A monoclonal antibody raised against membrane proteins of Toxoplasma gondii with molecular weights of 35 and 21 kDa also reacts strongly and "specifically" with surface antigens of Pneumocystis carinii with molecular weights of 394.2 and 69 kDa when used in a direct immunofluorescence antibody test, on the one hand, a...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00930914

    authors: Hassl A,Müller WA,Aspöck H

    更新日期:1991-01-01 00:00:00

  • Survey for the presence of Naegleria fowleri amebae in lake water used to cool reactors at a nuclear power generating plant.

    abstract::Water from Lake Anna in Virginia, a lake that is used to cool reactors at a nuclear power plant and for recreational activities, was assessed for the presence of Naegleria fowleri, an ameba that causes primary amebic meningoencephalitis (PAM). This survey was undertaken because it has been reported that thermally enri...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-1275-y

    authors: Jamerson M,Remmers K,Cabral G,Marciano-Cabral F

    更新日期:2009-04-01 00:00:00

  • Trypanosoma cruzi: evaluation of (-)-cubebin derivatives activity in the messenger RNAs processing.

    abstract::No fully effective treatment has been developed since the discovery of Chagas' disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to n...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2275-x

    authors: Andrade e Silva ML,Cicarelli RM,Pauletti PM,Luz PP,Rezende KC,Januário AH,da Silva R,Pereira AC,Bastos JK,de Albuquerque S,Magalhães LG,Cunha WR

    更新日期:2011-08-01 00:00:00

  • Experimental cysticercosis and the intrahepatic venous systems in Rattus norvegicus.

    abstract::During the present investigation, alterations in the intrahepatic venous and portal systems in Rattus norvegicus infected with 250, 500, and 1000 Taenia taeniaeformis eggs were studied. The corrosion cast technique was employed, which provides an extrinsic opportunity for study of the three-dimensional orientation of ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050703

    authors: Preet S,Prakash S

    更新日期:2000-06-01 00:00:00

  • Cloning, molecular characterization of a 13-kDa antigen from Schistosoma japonicum, Sj13, a putative salivary diagnosis candidate for Schistosomiasis japonica.

    abstract::Saliva has been suggested as an easily accessible and a noninvasive diagnostic alternative for detection of antibodies. To identify and characterize Schistosoma japonicum (S. japonicum) antigens that are recognized by saliva of infected host, we have used a pool of saliva from infected patients to immunoscreen an egg ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-009-1575-x

    authors: Zhou YP,Wu ZD,Yang LL,Sun X,You X,Yu XB,Hu W,Zheng HQ,Lv ZY

    更新日期:2009-10-01 00:00:00

  • Japanese monkey (Macaca fuscata) with alveolar echinococcosis after treatment with albendazole for 10 years: serodiagnosis and determination of albendazole metabolites.

    abstract::In 1997, an outbreak of alveolar echinococcosis in Japanese monkeys (Macaca fuscata) occurred in a zoo in Hokkaido, Japan. Twelve infected monkeys from a colony (n = 57) were diagnosed serologically by Western blotting, and ultrasonography showed the presence of tumor-like tissue in the livers of nine monkeys. The 12 ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-009-1627-2

    authors: Yamano K,Kanetoshi A,Goto A,Kishimoto M,Kobayashi N,Fujimoto S,Yamada K

    更新日期:2009-12-01 00:00:00

  • Characterization and bioassay for larvicidal activity of Anacardium occidentale (cashew) shell waste fractions against dengue vector Aedes aegypti.

    abstract::Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of A...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4598-5

    authors: Torres RC,Garbo AG,Walde RZ

    更新日期:2015-10-01 00:00:00

  • Distribution of Anopheles mosquito species, their vectorial role and profiling of knock-down resistance mutations in Botswana.

    abstract::Knowledge of vector species composition and monitoring their change over time is critical to evaluate malaria transmission and assess control interventions. This is especially important in countries such as Botswana, where malaria transmission is subjected to fluctuations due to climate variability. Another important ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-020-06614-6

    authors: Kgoroebutswe TK,Ramatlho P,Reeder S,Makate N,Paganotti GM

    更新日期:2020-04-01 00:00:00

  • Seasonal variation of strongylosis in working donkeys of Ethiopia: a cross-sectional and longitudinal studies.

    abstract::Helminths are one of the major health problems of working donkeys, often with heavy worm burden and contributing to their early demise and/or reduction in their work output. Cross-sectional and longitudinal studies were conducted to investigate the current infection prevalence and level of strongyles infection donkeys...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5485-z

    authors: Dibaba MD,Getachew AM,Assefa Z,Fanta A,Etana M,Firew S,Goshu L,Burden F

    更新日期:2017-07-01 00:00:00

  • Analysis of gamasid mites (Acari: Mesostigmata) associated with the Asian house rat, Rattus tanezumi (Rodentia: Muridae) in Yunnan Province, southwest China.

    abstract::During a survey lasting from 1990 to 2008, we captured 4,113 Asian house rats, Rattus tanezumi Temminck 1844 (Rodentia: Muridae) from 28 counties of Yunnan Province in southwestern China. From these rats, a total of 19,304 gamasid mites (Acari: Mesostigmata) were collected and identified as comprising 50 different spe...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3354-y

    authors: Huang LQ,Guo XG,Speakman JR,Dong WG

    更新日期:2013-05-01 00:00:00

  • Do parasites influence behavioural traits of wild and hatchery-reared Murray cod, Maccullochella peelii?

    abstract::This study aimed to investigate the links between parasites and behavioural traits of juvenile Murray cod (Maccullochella peelii). The Murray cod is an endangered Australian freshwater fish for which restocking programs are in place and there is a growing human consumption market. However, little is known about the pa...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-020-07021-7

    authors: Shamsi S,Rogers L,Sales E,Kopf RK,Freire R

    更新日期:2021-01-06 00:00:00

  • Dose- and time-dependent functional and structural damage to the colon mucosa by Entamoeba histolytica trophozoite lysates.

    abstract::Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lys...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00931595

    authors: Navarro-García F,López-Revilla R,Tstusumi V

    更新日期:1993-01-01 00:00:00

  • Analysis of a novel cathepsin B circulating antigen and its response to drug treatment in Trichinella-infected mice.

    abstract::In this paper, we cloned a novel full-length cDNA that encodes a Trichinella spiralis cathepsin B-like protease gene (TsCPB) using 3'-RACE PCR. The recombinant mature TsCPB protein (rTsCPB) was then expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3497-x

    authors: Zhan JH,Yao JP,Liu W,Hu XC,Wu ZD,Zhou XW

    更新日期:2013-09-01 00:00:00

  • Variable effects of host characteristics on species richness of flea infracommunities in rodents from three continents.

    abstract::We studied the effect of host gender and body mass on species richness of flea infracommunities in nine rodent host species from three biomes (temperate zone of central Europe, desert of the Middle East and the tropics of East Africa). Using season- and species-specific generalized linear mixed models and controlling ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-3937-2

    authors: Kiffner C,Stanko M,Morand S,Khokhlova IS,Shenbrot GI,Laudisoit A,Leirs H,Hawlena H,Krasnov BR

    更新日期:2014-08-01 00:00:00

  • Genetic uniqueness of Cryptosporidium parvum from dairy calves in Colombia.

    abstract::Fecal specimens from 432 pre-weaned calves younger than 35 days were collected over a 2-year period (2010-2012) from 74 dairy cattle farms in the central area of Colombia. These samples were microscopically examined for the presence of Cryptosporidium oocysts, and positive specimens were selected for molecular examina...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-018-5818-6

    authors: Avendaño C,Ramo A,Vergara-Castiblanco C,Sánchez-Acedo C,Quílez J

    更新日期:2018-05-01 00:00:00

  • Therapeutic immunization with radio-attenuated Leishmania parasites through i.m. route revealed protection against the experimental murine visceral leishmaniasis.

    abstract::After our promising results from prophylactic and therapeutic study (i.p. route) with the radio-attenuated Leishmania donovani parasites against experimental murine visceral leishmaniasis, we prompted to check their therapeutic efficacy through i.m route. BALB/c mice were infected with highly virulent L. donovani para...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-012-2847-4

    authors: Datta S,Manna M,Khanra S,Ghosh M,Bhar R,Chakraborty A,Roy S

    更新日期:2012-07-01 00:00:00

  • Size-dependent pattern of metacercariae accumulation in Macomona liliana: the threshold for infection in a dead-end host.

    abstract::While bivalves can acquire trematode metacercariae over their lifetime, the rate at which this accumulation takes place is not necessarily linear. The present study found that the bivalve Macomona liliana acquires very few or no metacercariae until it reaches 30 mm in size, but thereafter the rate at which it becomes ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-1166-2

    authors: Leung TL,Poulin R

    更新日期:2008-12-01 00:00:00

  • Experimental chemotherapy of Schistosoma mansoni with praziquantel and oxamniquine: differential effect of single or combined formulations of drugs on various strains and on both sexes of the parasite.

    abstract::The susceptibility of two Venezuelan (YT and SM) and one Brazilian (BH) strain of Schistosoma mansoni to single oral doses of praziquantel (Pz; 250 or 500 mg/kg), oxamniquine (Ox; 40, 60, or 100 mg/kg) or to low-dose combinations of both drugs (33 mg/kg Pz and 25 mg/kg Ox; 66 mg/kg Pz and 12.5 mg/kg Ox; 250 mg/kg Pz a...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00931515

    authors: Delgado VS,Suárez DP,Cesari IM,Incani RN

    更新日期:1992-01-01 00:00:00

  • A case of human orotracheal myiasis caused by Wohlfahrtia magnifica.

    abstract::A case of orotracheal myiasis in an 80-year-old man in coma for 1 week in the intensive care unit of Baskent University Hospital, Ankara, Turkey, is reported. A number of larvae of Wohlfahrtia magnifica were recovered repeatedly from the mouth and from the intubation tube of the patient and were reared to adults to fa...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050203

    authors: Ciftçioğlu N,Altintaş K,Haberal M

    更新日期:1997-01-01 00:00:00